Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2013 / Cilt: 19 - Sayı: 0

Tavşanlarda heterotopic allojenik ve otojenik yumurtalık nakli: Morfolojik ve endokrin özelliklerinin değerlendirilmesi ve karşılaştırılması

Heterotopic allogenic and autogenic ovarian transplantation in rabbits: Assessment and comparison of the morphological and endocrine characteristics

Pages
79–85
DOI
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Our results showed that there is no remarkable difference between autogenic and allogeneic heterotopic freshly transplanted ovarian tissue, especially in terms of the FSH and P4 levels during a four week period.

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Objective & Hypothesis:The aim of this study was to evaluate and compare the endocrine function and morphological characteristics of subcutaneous heterotopic allogenic and autogenic ovarian transplantation in immune-suppressed rabbits.
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Key Findings & Data:Group I (allogenic group) underwent freshly subcutaneous allogeneic heterotopic transplantation (n=7) (from group III to group I).
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Conclusion & Impact:Our results showed that there is no remarkable difference between autogenic and allogeneic heterotopic freshly transplanted ovarian tissue, especially in terms of the FSH and P4 levels during a four week period.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to evaluate and compare the endocrine function and morphological characteristics of subcutaneous heterotopic allogenic and autogenic ovarian transplantation in immune-suppressed rabbits. Each group included seven rabbits. Group I (allogenic group) underwent freshly subcutaneous allogeneic heterotopic transplantation (n=7) (from group III to group I). Group II (autogenic group) underwent freshly subcutaneous autogenic heterotopic transplantation (n7).Group III was the donor group (n=7). The levels of serum follicle-stimulating hormone (FSH) were significantly lower during the 2nd week (P=0.017) than during the 3rd week in group I, and were significantly higher during the 4th week than at other times (P=0.001) in group II. The levels of serum 17-β estradiol (E2) were significantly lower during the 3rd week (P=0.008) than during the 1st and 2nd weeks in group I, and were significantly lower during the 4th week than at other times (P=0.001) in group II. The levels of serum progesterone (P4) were not vary significantly (P=0.441) in group I and were significantly higher during the 1st week than during the 3rd week (P=0.033) in group II according to the ANOVA. Histological examination was performed using light microscopy after staining with hematoxylin-eosin (HE). Our results showed that there is no remarkable difference between autogenic and allogeneic heterotopic freshly transplanted ovarian tissue, especially in terms of the FSH and P4 levels during a four week period. We found that autogenic and allogeneic freshly transplanted ovarian tissues had similar characteristics in terms of the endocrine and histological characteristics.

Özet

Bu çalışmanın amacı, immün sistemi baskılanmış tavşanlara subkutan heterotopik allojenik ve otojenik yumurtalık naklinin endokrin ve morfolojik özelliklerinin değerlendirilmesi ve karşılaştırılmasıdır. Her gruba 7 tavşan dahil edildi. Grup I (allojenik: n=7) taze subkutan heterotopik allojenik transplantasyon (grup III’den, grup I’e), grup II (otojenik: n=7) taze subkutan heterotopik otojenik transplantasyon (kendi ciltaltlarına) ve grup III donor grupları olarak belirlendi. Serum follikül stimülan hormone (FSH) düzeyleri, grup I’de, 2. haftada, 3. haftadaki değerlerden (P=0.017) anlamlı derecede daha düşüktü, ve grup II’de 4. haftadaki değerler, diğer haftalardaki değerlerden anlamlı olarak daha yüksekti (P=0.001). Serum 17-β estradiol (E2) düzeyleri, grup I’de, 3. haftada, 1. ve 2. haftalardaki değerlerden anlamlı derecede düşüktü (P=0.008) ve grup II’de 4. haftadaki değerler, diğer zamanlardaki değerlerden anlamlı derecede düşüktü (P=0.001). Serum progesterone (P4) düzeyleri, grup I’de, anlamlı farklılık göstermedi (P=0.441) ve grup II’de, 3. haftadaki değerlerden, 1. haftadaki değerler anlamlı olarak yüksekti, ANOVA testine göre (P=0.033). Histolojik inceleme Hematoksilen-eozin (HE) ile boyanarak ışık mikroskobu ile yapıldı. Bizim sonuçlarımız özellikle dört haftalık dönemde, FSH ve P4 düzeyleri açısından otojenik ve allojenik heterotopik taze nakledilen yumurtalık dokusu arasında anlamlı fark olmadığını göstermiştir. Sonuç olarak, otojenik ve allojenik taze nakledilen yumurtalık dokularının endokrin ve histolojik yönden benzer özelliklere sahip olduğu belirlenmiştir.

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Cite This Article

TEMUR, İ., ÜLKER, K., ERMUTLU, C. Ş., GÜL, A., HÜSEYİNOĞLU, Ü., CİHAN, M., ATAKiŞİ, O., SÖZMEN, M. (2013). Tavşanlarda heterotopic allojenik ve otojenik yumurtalık nakli: Morfolojik ve endokrin özelliklerinin değerlendirilmesi ve karşılaştırılması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 19 - Sayı: 0, 79–85. https://ajindex.com/articles/272906

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References (12)

  1. Meraz MM, Gracida CJ, Melchor JLO, Revilla CM, Buen ND, Aburto EM: Restoration of endocrine function and ovulation after a heterotopic ovarian transplant in the inguinal region of rabbits using a vascular microsurgical technique. Transplant Proc, 38, 952-957, 2006
  2. Petroianu A, Aberti LR, Vasconcellos LS: Morphologic, endocrinologic and natural pregnancy assessment of allogeneic ovarian orthotopic trans- plantation without a vascular pedicle in Rabbits. Eur J Obstet Gynecol Reprod Biol, 133 (1): 70-75, 2007
  3. Petroianu A, Alberti LR, Vasconcellos LS: Allogeneic ovarian orthotopic transplantation in Rabbits without a vascular pedicle: Morphological, endocrinologic, and Natural Pregnancy Assessment. Tranplant Proc, 38, 3092-3093, 2006
  4. Damous LL, Silva SM, Simoes RS, Morello RJ, Carbonel APF, Simoes MJ: Effect of remote ischemic preconditioning on rat estradiol serum levels and follicular development after ovarian transplantation. Transplant Proc, 41, 830-833, 2009
  5. Callejo J, Vilaseca S, Medina M, Salvador C, Valls C, Lailla JM: Inhibin and follicular development in heterotopical ovary transplants without vascular pedicle in syngeneic Lewis rats. Fertil Steril, 79 (1): 743-748, 2003
  6. Bedaiwy MA, Jeremias E, Gurunluoglu R, Hussein MR, Sieminanow M, Biscotti C: Restoration of ovarian function after auto-transplantation of intact frozen thawed sheep ovaries with micro vascular anastomosis. Fertil Steril, 79 (3): 594-602, 2003
  7. Imhof M, Bergmeister H, Lipovac M, Rudas M, Hofstetter G, Huber J: Orthotopic microvascular re-anastomosis of whole cryopreserved ovine ovaries resulting in pregnancy and life birth. Fertil Steril, 85 (1): 1208-1215, 2006
  8. Gunasena KT, Villines PM, Crister E, Crister John K: Live births after autologous transplant of cryopreserved mouse ovaries. Hum Reprod, 12 (1): 101-106, 1997
  9. Risvanli A, Timurkan H, Akpolat N, Gulacti I, Ulakoglu E: A study of ovarian auto-transplantation without a vascular pedicle in rats. J Assist Reprod Gen, 23 (11-12): 401-406, 2006
  10. Callejo J, Salvador C, Miralles A, Vilaseca S, Lailla JM, Balasch J: Long- term ovarian function evaluation after auto grafting by implantation with fresh and frozen-thawed human ovarian tissue. J Clin Endocrinol Metab, 86 (9): 4489-4494, 2001
  11. Lee DM, Yeoman RR, Battaglia DE, Stouffer R, Zelinski-Wooten MB, Fanton JW: Live birth after ovarian tissue transplant. Nature, 428, 137-138, 2004
  12. Kim SS, Hwang in-T, Lee Hoi-C: Heterotopic auto-transplan