Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2013 / Cilt: 19 - Sayı: 3

Determination of the activity of alpha naphthyl acetate esterase and acid phosphatase of peripheral blood lymphocytes in healty pregnant merino sheep

Merinos ırkı sağlıklı gebe koyunların perifer kan lenfositlerinde alfa naftil asetat esteraz ve asit fosfataz aktivitelerinin belirlenmesi

Pages
483–488
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was concluded that the data was obtained from this study was useful for understanding of the possible mechanisms of maternal tolerance.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study was performed to determine the effects of pregnancy on the activities of alpha naphthyl acetate esterase (ANAE) and acid phosphatase (ACP-ase) of the peripheral blood lymphocytes in pregnant Merino sheep.
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Key Findings & Data:Peripheral blood lymphocyte percentages were also estimated.
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Conclusion & Impact:It was concluded that the data was obtained from this study was useful for understanding of the possible mechanisms of maternal tolerance.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:gebelik · Merinos · asit fosfataz

Abstract

This study was performed to determine the effects of pregnancy on the activities of alpha naphthyl acetate esterase (ANAE) and acid phosphatase (ACP-ase) of the peripheral blood lymphocytes in pregnant Merino sheep. Peripheral blood lymphocyte percentages were also estimated. Periferal blood samples were taken from animals in six different gestational stages as non-pregnant control, in the first, the second, the third, the fourth and the fifth month of pregnancy. Each group was contained 20 animals. The lowest ANAE (+) lymphocytes percentage (63.5%) was determined in the first month of pregnancy whereas the highest null lymphocytes proportion (12.75%) was detected in the same gestational period. There were statistically decreases in the proportions of peripheral blood lymphocyte (42.9%) and the ACP-ase (+) lymphocytes (43.35%) in the last gestational stages. Although the posibble hormonal changes may cause the fluctuation of peripheral blood lymphocyte proportions in all gestational periods, the most distinctive changes were observed at the begining and at the end of the pregnancy. It was concluded that the data was obtained from this study was useful for understanding of the possible mechanisms of maternal tolerance.

Özet

Bu çalışma, Merinos ırkı koyunlarda gebeliğin perifer kan lenfositlerinin alfa naftil asetat esteraz (ANAE) ve asit fosfataz (ACP-az) aktiviteleri ile perifer kan lenfosit oranları üzerindeki etkilerinin belirlenmesi amacıyla yapıldı. Hayvanlardan her grupta 20’şer adet olacak şekilde gebe olmayan-kontrol, bir aylık, iki aylık, üç aylık, dört aylık ve beş aylık gebeler olmak üzere toplam 6 dönemde perifer kanlar alındı. Histolojik incelemeler sonucunda en düşük ANAE pozitif-lenfosit oranı (%63.5) bir aylık gebe koyunlarda tespit edilirken aynı dönemde null lenfosit sayısı da en yüksek seviyede (%12.75) belirlendi. Gebeliğin son dönemindeki koyunların perifer kan lenfosit (%42.9) ve ACP-az pozitif lenfosit (%43.35) oranlarında da istatistiksel olarak önemli düşüşler gözlendi. Tüm gebelik dönemlerinde perifer kan lenfosit oranlarında hormonal değişimlerin neden olduğu düşünülen dalgalanmalar olsa da en belirgin değişimler gebeliğin ilk ve son dönemlerinde gözlendi. Bu çalışmadan elde edilen bulguların maternal toleransın olası mekanizmalarının anlaşılmasına katkı sağlayabileceği sonucuna varıldı.

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Cite This Article

SUR, E., AYDIN, İ., ÖZNURLU, Y., ÖZAYDIN, T., ÇELİK, İ., KADIRALİEVA, N. (2013). Determination of the activity of alpha naphthyl acetate esterase and acid phosphatase of peripheral blood lymphocytes in healty pregnant merino sheep. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 19 - Sayı: 3, 483–488. https://ajindex.com/articles/272865

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