Detection of parvovirus B19 prevalance by real time PCR assay in pregnant women

Objective & Hypothesis:Objective: To investigate the prevalance of Parvovirus B19 which has a risk of transplacental transmission to foetus among pregnant women with a risk of abortion applying to our hospital.

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Key Findings & Data:Material and Method: Seventy nine sera and 33 amniotic fluid samples of 112 pregnant women applying to our Obstetrics and Gynecology autpatient for routine control with ultrasound examination had been detected for Parvovirus B19 with real-time PCR method.

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Conclusion & Impact:The study of maternal sera and amniotic fluid by real-time PCR method is more expressive than serological methods.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Gebelik · Yaygınlık · Polimeraz zincir reaksiyonu

Abstract

Objective: To investigate the prevalance of Parvovirus B19 which has a risk of transplacental transmission to foetus among pregnant women with a risk of abortion applying to our hospital. Material and Method: Seventy nine sera and 33 amniotic fluid samples of 112 pregnant women applying to our Obstetrics and Gynecology autpatient for routine control with ultrasound examination had been detected for Parvovirus B19 with real-time PCR method. Results: Seven of 112 pregnant women had abnormal ultrasound findings: one hidrops, two oligohidroamnios and in utero growth retardation, one polihidroamnios and in utero growth retardation, one hiperechogenic bowel and two corioid phlexus cysts. No positive result had been found by real-time PCR method in favour of Parvovirus B19. Conclusion: The prevalance of Parvovirus B19 infection durind pregnancy is 3-4%. The risk of transplacental transmission to foetus is 25-33%. The study of maternal sera and amniotic fluid by real-time PCR method is more expressive than serological methods.

Özet

Amaç: Fetusa transplasental geçme riski olan enfeksiyon ajanlarından Parvovirus B19'un hastanemize başvuran düşük riskli gebelerdeki prevalansının araştırılmasıdır. Gereç ve Yöntem: Kadın Hastalıkları ve Doğum polikliniğine rutin kontrol amacıyla başvuran ve ultrsonografik muayenesi yapılan 112 gebeden alınan 79 kan, 33 amniyon sıvı örneğinde Real-Time PCR yöntemi kullanılarak Parvovirus B19 araştırılmıştır. Bulgular: Yüzoniki gebenin 7'sinde anormal ultrasonografik bulgulara rastlanmıştır; bir hidrops fetalis, iki oligohidramnios ve büyüme geriliği, bir polihidramnios ve büyüme geriliği, bir hiperekojenik barsak, iki korioid pleksusu kisti. Hastaların hiçbirinde Real Time PCR yöntemi ile pozitif sonuca rastlanmamıştır. Sonuç: Gebelikte Parvovirus B19 enfeksiyon sıklığı %3-4 arasındadır. Enfeksiyonun fetusa transplasental geçme riski %25-33'dir. Prenatal tanıda maternal kan ve amniyon sıvı örneğinin moleküler yöntemlerden Real Time PCR ile çalışılması seroloji çalışmalarına göre daha anlamlıdır.

Keywords

Gebelik Yaygınlık Polimeraz zincir reaksiyonu Parvovirus B19 insan Gebe kadınlar Pregnancy Prevalence Polymerase Chain Reaction Human Pregnant Women

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Cite This Article

BİRİ, A., BOZDAYI, G., KALKANCI, A., DİNÇ, B., HİMMETOĞLU, Ö., ROTA, S. (2004). Detection of parvovirus B19 prevalance by real time PCR assay in pregnant women. Medical Network Klinik Bilimler ve Doktor, Cilt: 10 - Sayı: 2, 200–203. https://ajindex.com/articles/272848

References (13)

Levy R, Weissman A, Blomberg G, Hagay ZJ. Infection by parvovirus B19 during pregnanacy: a review. Obstet Gynecol Surv 1997; 52:254-9.
Harger JH, Adler SP, Koch WC, Harger GF. Prospective (valuation of 618 pregnant women exposed to parvovirus B19:risk and symptoms. Obstet Gyneco 1998; 91:413-20.
Zerbini M, Musiani M, Gentilomi G, Venturoli S, Gallinella G, dorandi R. Comparative evaluation of virological and serological nethods in prenatal diagnosis of parvovirus B19 fetal hidrops. J îlin Microbiol 1996; 34:603-8.
Guidozzi F, Ballot D, Rothberg AD. Human B19 parvovirus infection in an obstetric population. A prospective study letermining fetal outcome. J Reprol Med 1994; 39:36-8.
Heegaard ED, Kevin BE. Human parvovirus B19. Clin dicrobiol Rew2002; 15:485-505.
Dieck D, Schild RL, Hansmann M, Eis-Hübinger AM. Prenatal diagnosis of congenital parvovirus B19 infection: value of serological and PCR technicques in maternal and fetal serum. Prenatal Diagn 1999; 19:1119-23.
Petrikovsky BM, Baker D, Schneider E. Fetal hydrops secondary to human parvovirus infection in early pregnancy.Pregnant Diagn 1996; 16:342-4.
Koch WC, Harger JH, Barnstein B, Adler SP. Serologic and virologic evidence for frequent intrauterine transmission of human parvovirus B19 vith a primary maternal infection during pregnancy. Pediatr Infect Dis J 1998; 17:489-94.
Lowden E, Weinstein L. Unexpected second trimester pregnancy loss due to maternal parvovirus B19 infection. Southern Med J 1997; 90:702-4. Jordan JA. Identification of human parvovirus B19 in idiopathic nonimmun hydrops fetalis. Am J Obstet Gynecol 1996; 174:37-42.
H.Kovacs BW, Carlson DE, Shahbahrami B, ve ark. Prenatal diagnosis of human parvovirus B19 in nonimmun hydrops fetalis by polymerase chain reaction. Am J Obstet Gynecol 1992; 167:461-6.
Török TJ, Wang Qy, Gary GW, ve ark. Prenatal diagnosis of intrauterin infection with parvovirus B19 by the polymerase chain reaction technique. Clin Infect Dis 1992; 14:149-55. Özsan M. Parvoviruslar. Willke Topçu A, Söyletir G, Doğanay M (ed). infeksiyon hastalıkları ve Mikrobiyolojisi. Nobel Tıp Kitapevi, istanbul 2002:1217-23.
Nyman M, Tolfvenstam T, Petrsson K, Krassny C, Skjöldebrand-Sparre L, Broliden K. Detection of human parvovirus B19 infection in first trimester fetal loss. Obstet Gynecol 2002; 99:795-8.
Skjöldebrand-Sparre L, Tolfvenstam T, Papadogiannakis N, Wahren B, Broliden K, Nyman M. Parvovirus B19 infection: association with third-trimester intrauterine fetal death. Br