Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2013 / Cilt: 19 - Sayı: 6

Rat siyatik ve kuyruk motor sinirlerinin minimal invaziv iletim çalışması

Minimally invasive motor Nerve conduction study of the rat sciatic and tail nerves

Pages
943–948
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Similar results of previous in vitro and in vivo studies prove the objectivity and reliability of our nerve conduction technique by bipolar needle electrodes on the non-exposed nerves.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Human and veterinary scientific researchers widely use rats as experimental animals.
📊
Key Findings & Data:The mean motor nerve conduction velocity of the sciatic nerves was 58.90±5.07 m/s and of the tail nerves was 40.23±2.39 m/s.
💡
Conclusion & Impact:Similar results of previous in vitro and in vivo studies prove the objectivity and reliability of our nerve conduction technique by bipolar needle electrodes on the non-exposed nerves.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Human and veterinary scientific researchers widely use rats as experimental animals. The rat nerves conduction studies are used for investigations of neural tissue injury, neural regeneration, peripheral neuropathies etc. in experimental models. Nerve conduction studies on animals do not follow strict rules, as studies in humans. Various methods and lack of the single recognized technique of nerve conduction study on the animals often lead to difficulties in the interpretation and comparison of the results of similar studies. In the present study, we have described the minimally invasive motor nerve conduction study on the rat sciatic and tail nerves. Electrophysiological examination including nerve conduction velocity and compound muscle action potential amplitudes measurements were performed on six normal growing male adult Wistar Albino rats. The mean motor nerve conduction velocity of the sciatic nerves was 58.90±5.07 m/s and of the tail nerves was 40.23±2.39 m/s. The mean compound muscle action potential amplitudes of the sciatic and tail nerves were 17.91±6.75 mV and 1.89±0.49 mV, respectively. Similar results of previous in vitro and in vivo studies prove the objectivity and reliability of our nerve conduction technique by bipolar needle electrodes on the non-exposed nerves.

Özet

Beşeri ve veteriner bilimsel araştırmacılar, ratları deneysel hayvan olarak yaygınca kullanmaktadırlar. Rat sinir iletim çalışmaları sinir dokusu hasarı, sinir rejenerasyonu, periferik nöropati ve buna benzer deneysel araştırma modellerinde kullanılmaktadır. Hayvanlar üzerinde yapılan sinir iletim çalışmaları, insan çalışmalarından farklı olarak, katı kurallara uymaz. Hayvanlarda sinir iletim çalışmalarının çeşitli yöntemleri ve tek tanınan tekniğin olmayışı benzer çalışmaların sonuçlarının yorumlanmasında ve kıyaslanmasında zorluklara yol açar. Çalışmamızın amacı, rat siyatik ve kuyruk motor sinirlerinin minimal girişimsel iletim çalışma yöntemlerini tanımlamaktır. Altı erkek erişkin Wistar Albino ratın sinir iletim hızları ile bileşik kas aksiyon potansiyelleri ölçümlerini içeren elektrofizyolojik incelemesi yapıldı. Ortalama motor sinir iletim hızları siyatik sinirlerde 58.90±5.07 m/sn ve kuyruk sinirlerinde 40.23±2.39 m/sn idi. Siyatik ve kuyruk sinirlerin ortalama bileşik kas aksiyon potansiyellerinin genliği sırasıyla 17.91±6.75 mV ve 1.89±0.49 mV idi. Bipolar iğne elektrotlar ile ve sinir ekspozisyonu yapılmadan uyguladığımız bu sinir iletim tekniğimizin sonuçlarının daha önceki in vitro ve in vivo çalışmaların sonuçları ile benzerlik göstermesi bu tekniğinin objektiflik ve güvenilirliğini desteklemektedir.

🔖

Cite This Article

HÜSEYİNOĞLU, N., ÖZAYDIN, İ., HÜSEYİNOĞLU, Ü., YAYLA, S., AKSOY, Ö. (2013). Rat siyatik ve kuyruk motor sinirlerinin minimal invaziv iletim çalışması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 19 - Sayı: 6, 943–948. https://ajindex.com/articles/272804

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Hüseyinoğlu N, Özaydın İ, Yayla S, Yıldırım CH, Aksoy Ö, Kaya M, Şengöz A, Taşdemiroğlu E:Electrophysiological assessment of the effects of silicone tubes and hyaluronic acid on nerve regeneration in rats with sciatic neurorrhaphy. Kafkas Univ Vet Fak Derg, 18 (6): 917-922, 2012
  2. Wolthers M, Moldovan M, Binderup T, Schmalbruch H, Krarup C: Comparative electrophysiological, functional, and histological studies of nerve lesions in rats. Microsurg, 25 (6): 508-519, 2005
  3. Korte N, Schenk HC, Grothe C, Tipold A, Haastern- Talini K: Evaluation of periodic electrodiagnostic measurements to monitor motor recovery after different peripheral nerve lesions in the rat. Muscle Nerve, 44 (1): 63-73, 2011
  4. Oğuzhanoğlu A, Erdoğan C, Tabak E, Cenikli U: Comparison of conduction velocities of nerve fibers to smaller and larger muscles in rats. Int J Neurosci, 120 (1): 76-79, 2010
  5. Misumi J, Nagano M: Experimental study on the enhancement of the neurotoxicity of methyl n-butyl ketone by non-neurotoxic aliphatic monoketones. Br J Ind Med, 42 (3): 155-161, 1985
  6. Kasselman LJ, Veves A, Gibbons CH, Rutkove SB: Cold exposure exacerbates the development of diabetic polyneuropathy in the rat. Exp Diabetes Res, 2009:827943, E-pub 2010 Jan 14, DOI: 10.1155/2009/827943
  7. Schmalbruch H: Fiber composition of the rat sciatic nevre. Anat Rec, 215 (1): 71-81, 1986
  8. Canta A, Meregalli C, Chiorazzi A, Carozzi VA, Crippa L, Marmiroli P, Cavaletti G: The ventral caudal nerve: a physiologic-morphometric study in three different rat strains. J Peripher Nerv Syst 15 (2): 140-146, 2010
  9. Ulman YI, Ulus IH, Ozpinar A, Genc V: Preliminary notes for ethical conduct of animal experimentation with special reference to studies in Turkey. Kafkas Univ Vet Fak Derg, 17 (6): 1051-1056, 2011
  10. Russell W, Burch R: The principles of humane experimental technique, Great Britain 1959; cited by Flecknell P: Replacement, reduction and refinement. Altex, 19 (2): 73-78, 2002
  11. Ülker K, Hüseyinoglu Ü: Animal ethics and animal use in laparoscopic surgery. KJMS, 2 (3): 89-93, 2012
  12. Siconolfi LB, Seeds NW: Mice lacking tPA, uPA, or plasminogen genes showed delayed functional recovery after sciatic nerve crush. J Neurosci, 21 (12): 4348-4355, 2001
  13. Oh SJ: Nerve conduction studies. In, Oh SJ (Ed): Clinical Electromyography. 3rd ed., Lippicott, Williams and Wilkins, Philadelphia, 2003
  14. Wang KK, Nemeth IR, Seckel BR, Chakalis- Halley DP, Swann DA, Kuo JW, Bryan DJ, Cetrulo CL: Hyal