Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2014 / Cilt: 20 - Sayı: 1

The proportion and the distribution of t-lymphocytes, null lymphocytes and acid phosphatase positive lymphocytes of the peripheral blood and endometrium in pregnant mice

Gebe farelerde perifer kan ve endometriyum dokusunda t-lenfosit, null lenfosit ve asit fosfataz pozitif lenfositlerin oran ve dağılımları

Pages
135–141
DOI
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⚡ TL;DR(Too Long; Didn't Read)

It was concluded that the number and the distribution of the lymphocyte, T-lymphocyte, null lymphocyte, and ACP-ase positive lymphocyte were affected by pregnancy although there were some differences among the gestational periods.

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Objective & Hypothesis:This study was performed to determine the effects of pregnancy on the proportion of T-lymphocytes, null lymphocytes and acid phosphatase (ACP-ase) positive peripheral blood lymphocytes and the distribution of the mentioned cells in the decidua basalis region of endometrium in the pregnant mice.
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Key Findings & Data:For this purpose, mice at 12-14 weeks of age were divided into four groups as non-pregnant control, and at the middle of the first, second, and the third week of the pregnancy, corresponding to early, middle, and late (3rd, 10th, and 17th days of pregnancy) gestational stages respectively (n = 6 for each group).
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Conclusion & Impact:It was concluded that the number and the distribution of the lymphocyte, T-lymphocyte, null lymphocyte, and ACP-ase positive lymphocyte were affected by pregnancy although there were some differences among the gestational periods.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

This study was performed to determine the effects of pregnancy on the proportion of T-lymphocytes, null lymphocytes and acid phosphatase (ACP-ase) positive peripheral blood lymphocytes and the distribution of the mentioned cells in the decidua basalis region of endometrium in the pregnant mice. For this purpose, mice at 12-14 weeks of age were divided into four groups as non-pregnant control, and at the middle of the first, second, and the third week of the pregnancy, corresponding to early, middle, and late (3rd, 10th, and 17th days of pregnancy) gestational stages respectively (n = 6 for each group). The lowest T-lymphocytes percentage was determined at early and middle pregnancy in the peripheral blood (43.83%) and decidua basalis (10.83 number/0.1 mm2), respectively. There was a statistically significant increase in the proportions of the peripheral blood ACP-ase (+) lymphocytes (44.33%) at the mid-gestational period while the lowest ACP-ase positive lymphocyte numbers (5.50 number/0.1 mm2) in the decidua basalis was observed at early pregnancy. The highest null cell rates were found at early gestation either in the peripheral blood (11.50%) or in the decidua basalis (7.83 umber/0.1 mm2). The lowest percentage of peripheral blood lymphocyte (56.00%) was recorded at the early pregnancy. It was concluded that the number and the distribution of the lymphocyte, T-lymphocyte, null lymphocyte, and ACP-ase positive lymphocyte were affected by pregnancy although there were some differences among the gestational periods.

Özet

Bu çalışma, farelerde gebeliğin T-lenfosit, null lenfosit ve asit fosfataz (ACP-az) pozitif lenfositlerin perifer kandaki oranı ve endometriyumun desidua bazalis bölgesindeki dağılımı üzerindeki etkilerinin belirlenmesi amacıyla yapıldı. Bu amaçla, 12-14 haftalık fareler, gebe olmayan-kontrol grubu ile gebeliğin birinci, ikinci ve üçüncü haftalarının ortasına karşılık gelecek şekilde erken, orta ve geç gebelik dönemi (sırasıyla gebeliğin 3., 10. ve 17. günleri) olmak üzere 4 gruba ayrıldılar (n = 6). En düşük T-lenfosit oranı perifer kanda (%43.83) ve desidua bazalis dokusunda (10.83 adet/0.1 mm2) sırasıyla gebeliğin erken ve orta dönemlerinde tespit edildi. Perifer kan ACP-az pozitif lenfosit oranlarında gebeliğin ikinci haftasında istatistiksel olarak önemli bir yükselme gözlenirken (%44.33); desidua bazalis dokusunda en düşük ACP-az pozitif lenfosit sayısı (5.50 adet/0.1 mm2) gebeliğin erken döneminde gözlendi. Hem perifer kanda (%11.50) ve hem de desidua bazalis dokusunda (7.83 adet/0.1 mm2) en yüksek null hücre oranı erken gebelik döneminde tespit edildi. En düşük perifer kan lenfosit oranı (%56.00) erken dönemde gözlendi. Dönemler arasında bazı farklar olsa da gebeliğin lenfosit, T-lenfosit, null lenfosit ve ACP-az pozitif lenfositlerinin sayı ve dağılımlarını etkilediği sonucuna varıldı.

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Cite This Article

SUR, E., ÖZNURLU, Y., ÖZAYDIN, T., ÇELİK, İ., AYDIN, İ., KADIRALİEVA, N. (2014). The proportion and the distribution of t-lymphocytes, null lymphocytes and acid phosphatase positive lymphocytes of the peripheral blood and endometrium in pregnant mice. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 20 - Sayı: 1, 135–141. https://ajindex.com/articles/272782

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References (15)

  1. Ostensen M, Sicher P, Förger F, Villiger PM: Activation markers of periferal blood mononuclear cells in late pregnancy and after delivery: A pilot study. Ann Rheum Dis, 64, 318-320, 2005
  2. Moffet A, Loke C: Immunology of placentation in eutherian mammals. Nature Rev, 6, 584-594, 2006
  3. Mahmoud F, Abul H, Omu A, Al-Rayes S, Haines D, Whaley K: Pregnacy-associated changes in peripheral blood lymphoctye subpopulations in normal Kuwaiti women. Gynecol Obstet Invest, 52, 232- 236, 2001
  4. Medina KL, Smithson G, Kincade PW: Suppression of B lymphopoiesis during normal pregnancy. J Exp Med, 178, 1507-1515, 1993
  5. Nakamura N, Miyazaki K, Kitano Y, Fujisaki S, Okamura H: Supression of cytotoxic-T-lymphocyte activity during human pregnancy. J Reprod Immunol, 23 (2): 119-130, 1993
  6. Martinez CM, Buendia AJ, Sanchez J, Navarro JA: Immuno- phenotypical characterization of lymphocyte subpopulations of the uterus of non-pregnant and pregnant goats. Anat Histol Embryol, 34, 240- 246, 2005
  7. Knowles DM, Hoffman HT, Ferrarini M, Kunkel HG: The demonstration of acid α-naphthyl acetate esterase activity in human lymphocytes: Usefulness as a T-cell marker. Cell Immunol, 35, 112-123, 1978
  8. Çelik İ, Aştı RN, Ergene N: İnsan perifer kanındaki B, T ve Null lenfositlerinin esteraz sitokimyası ve yüzey immünoglobülinlerinin immünoenzimatik yöntemle boyanarak belirlenmesi. SÜ Tıp Fak Derg, 7 (4): 497-503, 1991
  9. Kajikawa O, Koyama H, Yashikawa T, Tsubaki S, Saito H: Use of alpha-naphthyl acetate esterase staining to identify T lymphocytes in cattle. Am J Vet Res, 44 (8): 1549-1552, 1983
  10. Maiti NK, Saini SS, Sharma SN: Histochemical studies on chicken peripheral blood lymphocytes. Vet Res Commun, 14, 207-210, 1990
  11. Wulff JC, Sale GE, Deeg HJ, Storb R: Nonspesific acid esterase activity as a marker for canine T-lymphocytes. Exp Hematol, 9 (8): 85-870, 1981
  12. Mueller J, Brundel RG, Buerki H, Keller HU, Hess MW, Cottier H: Nonspesific acid esterase activity: A criterion for differentiation of T and B lymphocytes in mouse lymph nodes. Eur J Immunol, 5, 270-274, 1975
  13. Basso G, Cocito MG, Semenzato G, Pezzutto A, Zanesco L: Cytochemical study of thymocytes and T lymphocytes. Br J Haematol, 44, 577-582, 1980
  14. Sur E, Aydın İ, Öznurlu Y, Telatar T, Çelik İ: Sağlıklı gebe sığırların perifer kan lenfositlerinde alfa naftil asetat esteraz ve asit fosfataz aktivitelerinin belirlenmesi. Vet Bil Derg, 24 (2): 5-12, 2008
  15. Dönmez HH, Eken E, Beşoluk K, Sur E: