Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2014 / Cilt: 20 - Sayı: 5

Koçlarda taze sperma parametreleri ve DNA bütünlüğü arasındaki ilişkilerin araştırılması

Investigation of relationships between DNA integrity and fresh semen parameters in rams

Pages
793–798
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Hence, DNA structure evaluation of sperm might be a useful tool for accurate prediction of the male fertility in individual rams.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of the present study was to evaluate the correlation between the routine semen analyses and sperm DNA integrity, as assesed by TUNEL in fresh semen of rams in Kivircik and Awassi breeds in breeding season.
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Key Findings & Data:For that purpose, 50 fresh ejaculates from 6 Kivircik and 4 Awassi rams were evaluated.
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Conclusion & Impact:Hence, DNA structure evaluation of sperm might be a useful tool for accurate prediction of the male fertility in individual rams.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of the present study was to evaluate the correlation between the routine semen analyses and sperm DNA integrity, as assesed by TUNEL in fresh semen of rams in Kivircik and Awassi breeds in breeding season. Semen was collected by electro-ejaculation five times every other day. For that purpose, 50 fresh ejaculates from 6 Kivircik and 4 Awassi rams were evaluated. The mean percentages of semen volume, concentration, mass activity, motility, acrosomal defects, HOST and DNA fragmentation of Kivircik and Awassi rams were 1.3 ml, 1.9 x109, 3.3 (0-5 scale), 72.6%, 13.2%, 73.6%, 16.2% and 1.0 ml, 1.9 x109, 3.3 (0-5 scale), 72.5%, 11.1%, 77.9%, 15.9%; respectively (P>0.05). Sperm DNA fragmentation was correlated adversely with semen volume (r = -0.329, P<0.05), concentration (r = -0.188, P>0.05), mass activity (r = -0.349, P<0.05), motility (r = -0.448, P<0.01), acrosomal defects (r = -0.103, P>0.05) and HOS test (r = -0.513, P<0.01). This study provided clear evidence that most of the parameters evaluated herein related to DNA fragmentation at one point or the other. Hence, DNA structure evaluation of sperm might be a useful tool for accurate prediction of the male fertility in individual rams.

Özet

Bu çalışmanın amacı, Kıvırcık ve İvesi ırkı koçlardan alınan spermaların, sezon içerisinde rutin sperma analizleri ve TUNEL ile yapılan sperm DNA bütünlüğü arasındaki korelasyonu değerlendirmektir. Sperma elektro-ejakülasyon yöntemiyle 5 kez ve birer gün aralıklarla alındı. Bu amaçla; 6 baş Kıvırcık ve 4 baş İvesi ırkı koçtan alınan 50 ejakülat değerlendirildi. Kıvırcık ve İvesi ırkı koçların ortalama sperma hacmi, konsantrasyonu, mass aktivitesi, motilitesi, akrozomal bozukluğu, HOST ve DNA fragmantasyonu yüzdeleri sırasıyla 1.3 ml, 1.9 x109, 3.3 (0-5 skala), %72.6, %13.2, %73.6, %16.2 ve 1.0 ml, 1.9 x109, 3.3 (0-5 skala), %72.5, %11.1, %77.9 ve %15.9 olarak bulundu. Sperm DNA fragmantasyonu; sperma hacmi (r = -0.329, P 0.05), mass aktivite (r = -0.349, P 0.05) ve HOS testi (r= -0.513, P<0.01) ile negatif korelasyon gösterdi. Bu çalışma, değerlendirilen çoğu parametrenin DNA fragmantasyonu ile bağlantılı olduğunu açıkça gösterdi. Dolayısıyla, DNA yapısının incelenmesi her bir koç ejakülatının fertilitesinin doğru olarak tespit edilmesi konusunda faydalı olabilir.

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Cite This Article

ALÇAY, S., TOKER, B., ÜSTÜNER, B., NUR, Z., SAĞIRKAYA, H., SOYLU, M. K. (2014). Koçlarda taze sperma parametreleri ve DNA bütünlüğü arasındaki ilişkilerin araştırılması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 20 - Sayı: 5, 793–798. https://ajindex.com/articles/272768

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