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Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2014 / Cilt: 20 - Sayı: 4

Nonylphenol‘ün dondurulmuş-çözdürülmüş boğa sperma hareket kinetiğine, akrozom ve mitokondrial membran potansiyeline etkisi

Effects of nonylphenol on motion kinetics, acrosome and mitochondrial membrane potential in frozen-thawed bull sperm

CEVDET UĞUZ
ÖMER VARIŞLI
Cansu AĞCA
Yüksel AĞCA
Pages
583–590
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, NP impairs the sperm characteristics by adversely affecting motion kinetics, mitochondrial membrane potential and inducing premature acrosome reaction in bovine.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Recent reports have shown that the Nonylphenol (NP), a well-known toxic environmental chemical has adverse effects on sperm in different species.
📊
Key Findings & Data:Sperm were exposed to different concentrations of NP (1, 10, 100, 250 and 500 μg NP/mL) dissolved either in DMSO or Ethanol and incubated for 4 h at 37oC.
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Conclusion & Impact:In conclusion, NP impairs the sperm characteristics by adversely affecting motion kinetics, mitochondrial membrane potential and inducing premature acrosome reaction in bovine.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Recent reports have shown that the Nonylphenol (NP), a well-known toxic environmental chemical has adverse effects on sperm in different species. Therefore, the current study was aimed to determine the deleterious effect of NP on frozen-thawed bull sperm. Sperm were exposed to different concentrations of NP (1, 10, 100, 250 and 500 &#956;g NP/mL) dissolved either in DMSO or Ethanol and incubated for 4 h at 37oC. Sperm parameters were assessed at 0, 1st, 2nd, 3rd, and 4th h of incubation. Both percent motility and mitochondrial membrane potential (MMP) of sperm have decreased significantly (P<0.05) at dose level of &#8805; 250 &#956;g NP/mL. Acrosome reaction was induced (P<0.05) even at minimal dose concentrations of 1&#956;g NP/mL (P<0.05). However, the percentage of acrosome reacted sperm increased (P<0.05) with increase in dose level. In conclusion, NP impairs the sperm characteristics by adversely affecting motion kinetics, mitochondrial membrane potential and inducing premature acrosome reaction in bovine.

Özet

Son çalışmalar çevre toksini olarak bilinen Nonylpfenol (NP)’in, değişik türlerin sperm parametreleri üzerine zararlı etkisinin olduğunu göstermiştir. Bu çalışma NP’in dondurulmuş-çözdürülmüş boğa sperması üzerine etkisinin tespit edilmesi amacı ile yapılmıştır. Sperma örnekleri Ethanol veya DMSO içerinde değişik yoğunlukta (1, 10, 100, 250 ve 500 μg/ml) çözdürülen NP ile 4 saat 37oC’de inkübe edildi. Sperm parametreleri inkübasyonun 0., 1., 2., 3. ve 4. saatlerinde ölçüldü. Hem motilite ve hem de mitokondrial membran potansiyeli 250 μg NP/ml ≥ dozlara sahip gruplarda önemli derecede düşmüştür (P<0.05). Minimal doz olan 1μg/ml NP’de akrozom reaksiyonunu indüklenmiştir (P<0.05), ancak dozun artmasına bağlı olarak akrozom reaksiyonu geçirmiş sperm oranında da artışlar olduğu tespit edilmiştir (P<0.05). Sonuç olarak NP; spermatozoa hareket kinetiği, mitokondrial membran potansiyeli ve akrozom reaksiyonu üzerine negatif etki yaparak sperma parametrelerine zarar verdiği saptanmıştır.

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Cite This Article

UĞUZ, C., VARIŞLI, Ö., AĞCA, C., AĞCA, Y. (2014). Nonylphenol‘ün dondurulmuş-çözdürülmüş boğa sperma hareket kinetiğine, akrozom ve mitokondrial membran potansiyeline etkisi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 20 - Sayı: 4, 583–590. https://ajindex.com/articles/272679

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