Tavuklarda Genişlemiş Spektrumlu ?-laktamaz ve AmpC ?-laktamaz üreten Escherichia coli İzolatlarının Saptanması

Objective & Hypothesis:The aim of this study was to investigate the presence of Extended-spectrum ?-lactamase (ESBL) and AmpC ?-lactamase producing Escherichia coli strains isolated from meat and faecal samples of chicken.

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Key Findings & Data:Faecal samples (n: 384) were collected from broiler and egg-type healthy chickens in 43 flocks and 384 fresh chicken meat samples from local markets, supermarkets and slaughterhouses.

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Conclusion & Impact:The only plasmidic AmpC ?-lactamase found was the CIT type.

🔬Design:Research Article

📍Scope:2013 Verileri

⚙️Variables:Domain Parameters

Abstract

The aim of this study was to investigate the presence of Extended-spectrum ?-lactamase (ESBL) and AmpC ?-lactamase producing Escherichia coli strains isolated from meat and faecal samples of chicken. Faecal samples (n: 384) were collected from broiler and egg-type healthy chickens in 43 flocks and 384 fresh chicken meat samples from local markets, supermarkets and slaughterhouses. E. coli isolates were tested phenotypically according to the guidelines of CLSI (2013) for ESBL production. Phenotypic detection of AmpC production was carried out by determination of resistance to cefoxitin and susceptibility to cefepime. Also, presence of genes encoding different types of ?-lactamases (blaCTX-M, blaTEM, blaSHV and the mutation in the promoter of AmpC) in all phenotypically ESBL or AmpC producing E. coli was investigated by PCR assays. In this study, blaCTX-M (95.4%), blaCTX-M-1 (81.8%), blaCTX-M-8 (4.5%), blaTEM (45.4%) and blaSHV (2.2%) genes were detected from faecal samples and blaCTX-M (100%), blaCTX-M-1 (28.5%) and blaTEM (7.1%) from meat samples. The only plasmidic AmpC ?-lactamase found was the CIT type.

Özet

Bu çalışmanın amacı, tavuk eti ve dışkı örneklerinden izole edilen Genişlemiş Spektrumlu ?-laktamaz (GSBL) ve AmpC ?-laktamaz üreten Escherichia coli izolatlarının varlığını ortaya koymaktır. Bu çalışmada, 43 kümesten sağlıklı tavuklara ait 384 dışkı örneği ve yerel marketlerden, süpermarketlerden ve kesimhanelerden 384 taze tavuk eti örneği toplandı. E. coli izolatları fenotipik olarak CLSI (2013) standartları kullanılarak GSBL üretimi yönünden incelendi. AmpC üretiminin fenotipik olarak saptanması sefoksitine direnç ve sefepime duyarlılık kriterleri ile belirlendi. Fenotipik olarak GSBL ya da AmpC üreten E. coli olarak saptanan izolatlarının hepsi PCR ile değişik tipteki ?-laktamaz genlerinin (blaCTX-M, blaTEM, blaSHV, AmpC) varlığını ortaya koymak için incelendi. Bu çalışmada, dışkı örneklerinde blaCTX-M (%95.4), blaCTX-M-1 (%81.8), blaCTX-M-8 (%4.5), blaTEM (%45.4), blaSHV (%2.2) genleri ve tavuk eti örneklerinde ise blaCTX-M(%100), blaCTX-M-1 (%28.5), blaTEM (%7.1) genleri saptandı. AmpC ?-laktamaz grubunda sadece CIT geni belirlendi.

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Cite This Article

KAHRAMAN, B. B., SIĞIRCI, B. D., ÇELİK, B., GÜMÜŞ, B., METİNER, K., ADIGÜZEL, M. C., BAĞCIGİL, A. F., İKİZ, S., ÖZGÜR, N. Y., AK, S. (2016). Tavuklarda Genişlemiş Spektrumlu ?-laktamaz ve AmpC ?-laktamaz üreten Escherichia coli İzolatlarının Saptanması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 22 - Sayı: 4, 591–596. https://ajindex.com/articles/272505

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