Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2017 / Cilt: 23 - Sayı: 5

İnek Sütünde Koagulaz Pozitif Staphylococcus aureus Coa Geni Prevalansı ve İlgili Risk Faktörleri

The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk

Pages
809–815
DOI
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⚡ TL;DR(Too Long; Didn't Read)

The overall prevalence of subclinical mastitis was 55% comprising 54 and 56% in cattle and buffalo’s milk, respectively.

📌 3 Key Takeaways

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Objective & Hypothesis:The study describes prevalence of coagulase positive Staphylococcus aureus, a zoonotic pathogen, and rule out key risk factors associated with the spread of this pathogen.
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Key Findings & Data:A total of 900 milk samples of animals (n = 450 cattle and n = 450 buffaloes) from three subdistricts of Faisalabad were collected and processed microbiologically for staphylococci identification while, PCR for coa gene identification.
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Conclusion & Impact:The overall prevalence of subclinical mastitis was 55% comprising 54 and 56% in cattle and buffalo’s milk, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The study describes prevalence of coagulase positive Staphylococcus aureus, a zoonotic pathogen, and rule out key risk factors associated with the spread of this pathogen. A total of 900 milk samples of animals (n = 450 cattle and n = 450 buffaloes) from three subdistricts of Faisalabad were collected and processed microbiologically for staphylococci identification while, PCR for coa gene identification. Surf field mastitis test was used to screen the samples for subclinical mastitis at milking. Chi-square test was used to assess association of risk factors with mastitis. The overall prevalence of subclinical mastitis was 55% comprising 54 and 56% in cattle and buffalo’s milk, respectively. A significant difference (P<0.05) was seen in the prevalence of subclinical mastitis among the cattle from different cities. The prevalence of coa gene presented 39.33% from total samples. Highest prevalence was found in tehsil Samundari followed by Jaranwala and Faisalabad presenting 43%, 39%, and 36%, respectively. The quarter based prevalence of subclinical mastitis was found 32% while 5.58% of quarters were blind. Right side quarters and teats were more affected. The risk factors except body condition and parity presented significant association with mastitis. The study concluded that bacterial coa gene, was prevalent in milk, and determinants were strongly associated with higher prevalence

Özet

Bu çalışma; zoonotik bir patojen olan koagülaz pozitif Staphylococcus aureus'un prevalansını ve bu patojenin yayılmasında rol oynayan risk fakörlerini tanımlamaktadır. Faisalabad'ın 3 ayrı bölgesindeki hayvanlardan toplanan toplam 900 adet süt örneğinde (n = 450 sığır ve n = 450 manda) Staphylococci identifikasyonu amacıyla mikrobiyolojik analiz ve coa geninin tespiti amacıyla PCR gerçekleştirildi. Subklinik mastitisin taranmasında sağım esnasında sörf mastitis testi uygulandı. Risk faktörlerinin mastitisle ilişkisini değerlendirmek amacıyla Ki-kare testi kullanıldı. Sığır ve manda sütlerinde subklinik mastitis prevalansı sırasıyla %54 ve %56'sı olarak hesaplanırken toplamda %55 olarak bulundu. Farklı şehirlerden örneklenen sığırlar arasında subklinik mastitis prevalansı yönünden anlamlı bir fark tespit edildi (P<0.05). Coa geninin prevalansı toplam örneklerde %39.33 olarak saptanmıştır. En yüksek prevalans oranı %43 olarak Samundari'de saptanırken bunu sırasıyla %39 ile Jaranwala ve %36 ile Faisalabad izlemektedir. Subklinik mastitisin meme loblarındaki prevalansı %32 olarak saptanırken meme loblarının %5.58'inde ise körelme belirlendi. Sağ taraftaki meme lobları ve meme başlarının daha fazla etkilendiği saptandı. Vücut kondisyonu ve doğum sayısı dışındaki risk faktörleri mastitis ile anlamlı ilişki içeriyordu. Çalışma, bakteriyel coa geninin sütte yaygınlığını ve göstergelerin yüksek prevalans ile kuvvetli bir ilişkisi olduğunu göstermektedir

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Cite This Article

MALIK, M. A. R., IJAZ, M., AQIB, A. I., FAROOQI, S. H., HUSSAIN, K. (2017). İnek Sütünde Koagulaz Pozitif Staphylococcus aureus Coa Geni Prevalansı ve İlgili Risk Faktörleri. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 23 - Sayı: 5, 809–815. https://ajindex.com/articles/272343

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