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Journals / KONURALP TIP DERGİSİ / 2021 / Cilt: 13 - Sayı: 1

Evaluation of the Effects of Rapamycin Treatment on Antioxidant Enzyme Changes and AgNOR in Testicular Torsion

Testis Torsiyonunda Rapamisin Tedavisinin Antioksidan Enzim Değişiklikleri ve AgNOR Üzerindeki Etkilerinin Değerlendirilmesi

Murat KABAKLIOĞLU
Recep ERÖZ
Murat KAYA
Pages
45–54
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Thus it can be said that these proteins may be used in the development of new and more effective therapeutic approaches to prevent the negative effects of T/D injury.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective:Testicle torsion/detorsion cause ischemia.
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Key Findings & Data:Results: Significant differences were detected among the groups for mean AgNOR number and TAA/TNA(p<0.05).
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Conclusion & Impact:Thus it can be said that these proteins may be used in the development of new and more effective therapeutic approaches to prevent the negative effects of T/D injury.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Testis torsiyonu · NOR · AgNOR

Abstract

Objective:Testicle torsion/detorsion cause ischemia. Rapamycin has immune suppressive and antioxidant defense mechanisms. Nucleolar-organizing regions(NORs) are loops of ribosomal DNA. Methods: To evaluate mean AgNOR number and total AgNOR area/total nuclear area(TAA/TNA) ratio and the relation between these proteins and rapamycin in the Torsion/Detorsion process of testes. The six groups as control, sham, early and late torsion-detorsion (ETD<D) groups, and early and late rapamycin treatment groups (ETD+R<D+R) were included. The TAA/TNA and mean AgNOR number of testes cells and biochemical analysis of GPx, SOD, and TBARS activities were detected. Results: Significant differences were detected among the groups for mean AgNOR number and TAA/TNA(p<0.05). For both mean AgNOR number and TAA/TNA, significant differences were found between control and ETD, between control and ETD+R, between control and LTD, between control and LTD+R. Also, a statistically significant relationship between both mean AgNOR numbers and TAA/TNA of testes cells and all the antioxidant enzymes (SOD, TBARS, and GPX) were detected(p<0.05). Conclusion: We may obtain information about the levels and duration of testes injury considering the levels of these proteins. Thus it can be said that these proteins may be used in the development of new and more effective therapeutic approaches to prevent the negative effects of T/D injury.

Özet

Amaç: Testis torsiyonu / detorsiyonu iskemiye neden olur. Rapamisin, immün baskılayıcı ve antioksidan savunma mekanizmalarına sahiptir. Nükleolar düzenleyen bölgeler (NOR'ler) ribozomal DNA'nın döngüleridir. Yöntem: Testislerin Torsiyon / Detorsiyon işleminde ortalama AgNOR sayısı ve toplam AgNOR alanı / toplam nükleer alan (TAA / TNA) oranını ve bu proteinler ile rapamisin arasındaki ilişkiyi değerlendirmek. Kontrol, sahte, erken ve geç torsiyon-detorsiyon (ETD & LTD) grupları ve erken ve geç rapamisin tedavi grupları (ETD + R & LTD + R) olmak üzere altı grup dahil edildi. TAA / TNA ve testis hücrelerinin ortalama AgNOR sayısı ile GPx, SOD ve TBARS aktivitelerinin biyokimyasal analizi tespit edildi. Bulgular: Gruplar arasında ortalama AgNOR sayısı ve TAA / TNA açısından önemli farklılıklar tespit edildi (p <0.05). Hem ortalama AgNOR sayısı hem de TAA / TNA için, kontrol ile ETD arasında, kontrol ile ETD + R arasında, kontrol ile LTD arasında, kontrol ile LTD + R arasında önemli farklılıklar bulundu. Ayrıca testis hücrelerinin ortalama AgNOR sayıları ile TAA / TNA ile tüm antioksidan enzimler (SOD, TBARS ve GPX) arasında istatistiksel olarak anlamlı ilişki saptandı (p <0.05). Sonuç: Bu proteinlerin seviyelerine göre testis hasarının seviyeleri ve süresi hakkında bilgi edinebiliriz. Bu nedenle, bu proteinlerin, T / D hasarının olumsuz etkilerini önlemek için yeni ve daha etkili terapötik yaklaşımların geliştirilmesinde kullanılabileceği söylenebilir.

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Cite This Article

KABAKLIOĞLU, M., ERÖZ, R., KAYA, M. (2021). Evaluation of the Effects of Rapamycin Treatment on Antioxidant Enzyme Changes and AgNOR in Testicular Torsion. KONURALP TIP DERGİSİ, Cilt: 13 - Sayı: 1, 45–54. https://ajindex.com/articles/267665

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