AI Summary & Key Takeaways
AI InsightAlthough the studied edible plants were chosen as real samples, the developedLC-MS/MS method is applicable to a wide range of species in plant kingdom.
📌 3 Key Takeaways
Abstract
A comprehensive LC-MS/MS method was developed and validated for the quantification of eightplant phytochemicals (cynarin, caffeic acid, syringic acid, p-coumaric acid, o-coumaric acid, vanillic acid, ferulicacid, chlorogenic acid) in plants. The developed analytical method was fully validated in terms of linearity, accuracy(recovery), inter and intra-day precision (repeatability), limits of detection and quantification (LOD/LOQ) andrelative standard uncertainty (U% at 95% confidence level (k=2)). Chromatographic separation was performed ona reverse phased UHPLC. MS detection was performed using a triple quadrupole mass spectrometer and negativeor positive ionization modes were optimized for each analyte. Multiple reaction monitoring (MRM) was usedto quantify the analytes, related molecular ions and transition ions were optimized. After method validation, thephytochemical composition of methanolic extracts of some edible plants including artichoke (Cynara scolymus L.),broccoli (Brassica Oleracea var. Italica), cauliflower (Brassica Oleracea var. Botrytis) and tumble thistle (GundeliaTournefortii) were investigated by the developed and validated LC-MS/MS method. Among the analysed plants,artichoke was by far the richest one in terms of phenolics. Additionally, chlorogenic acid was the most abundantphenolic compound in all plants. Although the studied edible plants were chosen as real samples, the developedLC-MS/MS method is applicable to a wide range of species in plant kingdom.
Özet
Bitkilerde 8 fitokimyasal bileşiğin (sinarin, kafeik asit, sirinjik asit, p-kumarik asit, o-kumarik asit, vanilik