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Journals / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2017 / Cilt: 7 - Sayı: 3

Syntaxin4 and Synapsin3 mRNA Levels Induced by Long-Term Iron Toxicity in Rat (Rattus norvegicus)

Sıçan (Rattus norvegicus)’da Uzun Süreli Demir Toksisitesi ile Uyarılan Sintaksin4 ve Sinapsin3 mRNA Seviyeleri

Abdullah TUNÇ
Orhan ERDOĞAN
Pages
107–114
Publication Date
2017-09-30
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

While mRNA levelof the syntaxin4 gene was unchanged in the cortex, mRNA level of syntaxin4 decreased with 30 ppm and 300 ppmexposure in the hippocampus. mRNA level of synapsin3 gene in both cortex and hippocampus tissues increased inall treatment groups compared to control group.

📌 3 Key Takeaways

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Objective & Hypothesis:Although iron is necessary for brain tissue, its overload causes many metabolic disorders as wellas neurodegenerative disorders.
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Key Findings & Data:Syntaxin4 and synapsin3 are proteins associated with synaptic vesicles that playa role in neurotransmitter release.
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Conclusion & Impact:While mRNA levelof the syntaxin4 gene was unchanged in the cortex, mRNA level of syntaxin4 decreased with 30 ppm and 300 ppmexposure in the hippocampus. mRNA level of synapsin3 gene in both cortex and hippocampus tissues increased inall treatment groups compared to control group.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Demir birikimi · mRNA ifadesi · sintaksin4

Abstract

Although iron is necessary for brain tissue, its overload causes many metabolic disorders as wellas neurodegenerative disorders. Syntaxin4 and synapsin3 are proteins associated with synaptic vesicles that playa role in neurotransmitter release. In this study we investigated the effects of sublethal doses of ferric iron at 0.87ppm, 3 ppm, 30 ppm and 300 ppm on mRNA levels of genes coding syntaxin4 and synapsin3 proteins that functionin nerve cell signal transmission in cortex and hippocampus tissues of rats (Rattus norvegicus). While mRNA levelof the syntaxin4 gene was unchanged in the cortex, mRNA level of syntaxin4 decreased with 30 ppm and 300 ppmexposure in the hippocampus. mRNA level of synapsin3 gene in both cortex and hippocampus tissues increased inall treatment groups compared to control group.

Özet

Demir beyin dokusu için gerekli olmasına rağmen aşırı birikimi nörodejeneratif rahatsızlıkların yanındabirçok metabolik bozukluklara neden olmaktadır. Sintaksin4 ve sinapsin3 nörotransmitter salınımında rol oynayansinaptik veziküller ile ilişkili proteinlerdir. Biz bu çalışmada ferrik demirin (Fe3+) 0.87 ppm, 3 ppm, 30 ppm ve 300ppm subletal dozlarına maruz kalmış sıçanların (Rattus norvegicus) korteks ve hipokampus dokularındaki sinirhücre sinyal iletiminde fonksiyonu olan Sintaksin4 ve Synapsin3 proteinlerini kodlayan genlerin mRNA seviyeleriüzerine etkilerini araştırdık. Sintaksin4 geninin mRNA seviyesi korteks dokusunda değişmez iken hipokampusdokusunda 30 ppm ve 300 ppm maruziyeti ile mRNA seviyesi azalmıştır. Hem korteks hem de hipokampusdokularında sinapsin3 geni mRNA seviyesi artmıştır.

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Cite This Article

TUNÇ, A., ERDOĞAN, O. (2017). Syntaxin4 and Synapsin3 mRNA Levels Induced by Long-Term Iron Toxicity in Rat (Rattus norvegicus). Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 7 - Sayı: 3, 107–114. https://ajindex.com/articles/257071

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