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Journals / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2019 / Cilt: 9 - Sayı: 3

Investigation of Antioxidant and In Vitro Wound Healing Activity of Fulvic Acid

Özlem Sultan ASLANTÜRK
Tülay AŞKIN ÇELİK
Yusuf Mert SÖNMEZ
Pages
1316–1326
Publication Date
2019-09-01
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

However, it accelerated wound healing by increasing cell migration, especially in HaCaT cells. 1 and 10 μg mL-1 FA had a significant wound healing effect on BJ cells, and all concentrations of FA had a significant wound healing effect on HaCaT cells at the end of the 24 h treatments.

📌 3 Key Takeaways

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Objective & Hypothesis:This study aimed to investigate the antioxidant and in vitro wound healing activities of fulvic acid (FA) purified from Hüsamlar leonardite in Muğla/Turkey.
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Key Findings & Data:Purified FA was analyzed with FT/IR spectrophotometer and flame photometer for determining functional groups and cation impurities, respectively. 1, 5, 10, 20, 30, 50, 100 μg mL-1 concentrations of FA were tested for antioxidant activity (by using DPPH radical scavenging and H2O2 scavenging assays).
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Conclusion & Impact:However, it accelerated wound healing by increasing cell migration, especially in HaCaT cells. 1 and 10 μg mL-1 FA had a significant wound healing effect on BJ cells, and all concentrations of FA had a significant wound healing effect on HaCaT cells at the end of the 24 h treatments.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:antioxidant activity · cell proliferation · fulvic acid

Abstract

This study aimed to investigate the antioxidant and in vitro wound healing activities of fulvic acid (FA) purified from Hüsamlar leonardite in Muğla/Turkey. Purified FA was analyzed with FT/IR spectrophotometer and flame photometer for determining functional groups and cation impurities, respectively. 1, 5, 10, 20, 30, 50, 100 μg mL-1 concentrations of FA were tested for antioxidant activity (by using DPPH radical scavenging and H2O2 scavenging assays). Also, effect of FA on BJ human foreskin fibroblast and HaCaT spontaneously immortalized non-tumorigenic human keratinocyte cells proliferation was tested by in vitro MTT and WST-8 assays and on cell migration by wound healing assay (scratch assay). The results of the study show that the FA has a low DPPH radical scavenging activity, but it exhibited high H2O2 scavenging activity at low concentrations. The effect of FA on the proliferation of BJ and HaCaT cells varied according to the cell type, FA concentration and treatment time. However, it accelerated wound healing by increasing cell migration, especially in HaCaT cells. 1 and 10 μg mL-1 FA had a significant wound healing effect on BJ cells, and all concentrations of FA had a significant wound healing effect on HaCaT cells at the end of the 24 h treatments.

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Cite This Article

ASLANTÜRK, Ö. S., ÇELİK, T. A., SÖNMEZ, Y. M. (2019). Investigation of Antioxidant and In Vitro Wound Healing Activity of Fulvic Acid. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 9 - Sayı: 3, 1316–1326. https://ajindex.com/articles/256773

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References (10)

  1. Apak R, Gorinstein S, Bohm V, Schaich KM, Özyürek M, et al. 2013. Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure and Applied Chemistry, 85: 957-998. Arnao MB, 2000. Some methodological problems in the determination of antioxidant activity using chromogen radicals: a practice case. Tr Food Sc Technol, 11(11): 419-421.
  2. Ascione F, Vasaturo A, Caserta S, D’Esposito V, Formisano P, Guido S, 2016. Comparison between fibroblast wound healing and cell random migration assays in vitro. Experimental Cell Research, 347: 123-132.
  3. Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M, 2008. Growth factors and cytokines in wound healing. Wound Repair Regen., 16: 585-601.
  4. Brand-Williams W, Cuvelier ME, Berset C, 1995. Use of free radical method to evaluate antioxidant activity. Food Science and Technology, 28: 25-30.
  5. Braun-falco O, Plewig G, Wolff HH, Burgdorf WHC (Eds.). 2000.
  6. Dermatology. Second ed. Springer, Berlin/Heidelberg/Newyork. Cornejo A, Jiménez JM, Caballero L, Melo F, Maccioni RB, 2011. Fulvic acid inhibits aggregation and promotes disassembly of tau fibrils associated with Alzheimer’s disease. J Alzheimers Dis., 27: 143-153.
  7. De Donatis A, Cirri P, 2009. Understanding the specificity of receptor tyrosine kinases signaling. Commun Integr Biol., 1: 156-157.
  8. Ebeling S, Naumann K, Pollok S et al. 2014. From a traditional medicinal plant to rational drug: Understanding the clinically proven wound healing efficacy of birch bark extract. PLoS One, 9: e86147. Fronza M, Heinzmann B, Hamburger M et al., 2009. Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol., 126: 463-467.
  9. Gao Y, Zhu J, Bao H, Hector V, Zhao B, Chu Z, 2018. Effect of lignite fulvic acid on growth, antioxidant ability, and HSP70 of Pacific white shrimp, Litopenaeus vannamei. Aquaculture Iternational, 26(6): 1519-1530.
  10. Guo S, Di Pietro LA, 2006. Factors Affecting Wound Healing. J Dent Res., 89: 219-29. Guzmán-Martinez L, Farías GA, Maccioni RB, 2013. Tau oligomers as potential targets for Alzheimer’s diagnosis and novel drugs. Frony Neurol., 4: 167. Hoang AM, Oates TW, Cochran DL, 2000. In vitro wound healing responses to enamel matrix derivative. Journal of Periodontology, 71(8): 1270-1277. Jurcsik I, 1994. Possibilities of applying humic acids in medicine, wound healing and cancer therapy. In: Senesi N, Milano TM., editors. Humic Substances in the Globa