Journals / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2020 / Cilt: 10 - Sayı: 4

Determination of Antimicrobial and Antioxidant Activity of Some Spices Widely Consumed in Turkey

Türkiye’de Yaygın Bir Şekilde Tüketilen Bazı Baharatların Antioksidan ve Antimikrobiyal Aktivitesinin Belirlenmesi

Pages
2574–2582
Publication Date
2020-12-15
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

When the total phenolic concentrations of spice extracts ranged from 6239.58 to 27197.92 mg GAE/kg, the ABTS values ranged from 40.75 to 667.57 mmol Trolox/g dried spice and the percentage of DPPH inhibitions ranged from 88.98 to 96.26%.

📌 3 Key Takeaways

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Objective & Hypothesis:In the present study, antimicrobial and antioxidant properties of methanol extracts of some packaged and unpackaged spices including cinnamon (Cinnamomum zeylanicum), cumin (Cuminum cyminum L.), thyme (Thymus vulgaris L.) and mint (Mentha spicata L.) were determined.
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Key Findings & Data:While the antioxidant properties of spice extracts were examined using 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) methods, the total phenolic components were examined using Folin–Ciocalteu method.
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Conclusion & Impact:When the total phenolic concentrations of spice extracts ranged from 6239.58 to 27197.92 mg GAE/kg, the ABTS values ranged from 40.75 to 667.57 mmol Trolox/g dried spice and the percentage of DPPH inhibitions ranged from 88.98 to 96.26%.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Baharatlar · antioksidan kapasite · antibacterial etki

Abstract

In the present study, antimicrobial and antioxidant properties of methanol extracts of some packaged and unpackaged spices including cinnamon (Cinnamomum zeylanicum), cumin (Cuminum cyminum L.), thyme (Thymus vulgaris L.) and mint (Mentha spicata L.) were determined. The antimicrobial effect of different concentrations of these spice extracts was examined by agar diffusion method against Escherichia coli, Enterococcus faecalis, Bacillus cereus, Pseudomonas aeruginosa and Salmonella typhimurium. While the antioxidant properties of spice extracts were examined using 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) methods, the total phenolic components were examined using Folin–Ciocalteu method. Methanol extracts of spices showed antimicrobial activity at different rates against tested microorganisms. The methanol extract of unpackaged mint showed highest inhibiton zones (22.50 mm) against Salmonella typhimurium. When the total phenolic concentrations of spice extracts ranged from 6239.58 to 27197.92 mg GAE/kg, the ABTS values ranged from 40.75 to 667.57 mmol Trolox/g dried spice and the percentage of DPPH inhibitions ranged from 88.98 to 96.26%.

Özet

Bu çalışmada ambalajlanmış ve ambalajlanmamış tarçın (Cinnamomum zeylanicum), kimyon (Cuminum cyminum L.), kekik (Thymus vulgaris L.) ve nanenin (Mentha spicata L.) metanol ektraktlarının antimikrobiyal ve antioksidan özellikleri belirlenmiştir. Bu baharat ekstrelerinin Escherichia coli, Enterococcus faecalis, Bacillus cereus, Pseudomonas aeruginosa ve Salmonella typhimurium’a karşı antimikrobiyal etkisi agar difüzyon yöntemi ile belirlenmiştir. Baharat ekstrelerinin antioksidan özellikleri 2,2′-azino-bis-3-etilbenzotiazolin-6-sulfonik asit (ABTS) and 2,2-difenil-1-pikril-hidrazil (DPPH) yöntemleri kullanılarak incelenirken, toplam fenolik bileşikler Folin-Ciocalteu yöntemi kullanılarak incelenmiştir. Baharatların metanol ekstreleri test edilen mikroorganizmalara karşı farklı oranlarda antimikrobiyal aktivite göstermiştir. En yüksek inhibisyon aktiviteyi (22.50 mm) ambalajlanmamış nanenin metanol ekstresi Salmonella typhimurium'a karşı göstermiştir. Baharat özütlerinin toplam fenolik konsantrasyonları 6239.58 ile 27197.92 mg GAE / kg arasında değiştiğinde, ABTS değerlerinin 40.75 ile 667.57 mmol Trolox / g kurutulmuş baharat ve DPPH inhibisyon yüzdesinin % 88.98 ile % 96.26 arasında değiştiği saptanmıştır.

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Cite This Article

KÖSE, Ş. (2020). Determination of Antimicrobial and Antioxidant Activity of Some Spices Widely Consumed in Turkey. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 10 - Sayı: 4, 2574–2582. https://ajindex.com/articles/256411

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References (10)

  1. Aliakbarlu J, Mohammadi S, Khalili S, 2014. A Study on Antioxidant Potency and Antibacterial Activity of Water Extracts of Some Spices Widely Consumed in Iranian Diet. Journal of Food Biochemistry, 38: 159-166.
  2. Brand-Williams W, Cuvelier ME, Berset C, 1995. Use of a Free Radical Method to Evaluate Antioxidant Activity. LWT-Food Science and Technology, 28 (1): 25-30.
  3. Cai YZ, Luo Q, Sun M, Corke H. 2004. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences, 74: 2157–2184.
  4. Chan C-L, Gan R-Y, Corke H, 2016. The Phenolic Composition and Antioxidant Capacity of Soluble and Bound Extracts in Selected Dietary Spices and Medicinal Herbs. Journal of Food Science and Technology, 51:565–573.
  5. Cetin B, Cakmakci S, Cakmakci R, 2011. The Investigation of Antimicrobial Activity of Thyme and Oregano Essential Oils. Turkish Journal of Agriculture and Forestry, 35:145-154.
  6. Celikel N, Kavas G, 2008. Antimicrobial Properties of Some Essential Oils Against Some Pathogenic Microorganisms. Czech Journal of Food Sciences, 26: 174–181. Dagdelen S, (2010). Determination of Antimicrobial and Antioxidant Effects, Aroma Profile and Some Chemical Properties of Important Herbs Species Added to Otlu Cheese., Inonu University Graduate School of Natural and Applied Sciences Department of Food Engineering, PhD Thesis (Printed). Dagdelen S, Bilenler T, Durmaz G, Gokbulut I, Hayaloglu AA, Karabulut I, 2014.
  7. Volatile Composition, Antioxidant and Antimicrobial Activities of Herbal Plants Used In The Manufacture of Van Herby (Otlu) Cheese. Journal of Food Processing and Preservation, 38:1716-1725.
  8. Dalwadi K, Elias J, Patel VH, Subhash R, 2016. Comparative Study of Antioxidant Properties of Cinnamon and Kokum In Aqueous and Methanolic Extracts. Department of Higher Education-Govarnment of Gujarat Journal of Science, August-September, 2016.
  9. Deans SG, Svoboda KP, 1989. Antimicrobial Activity of Summer Savory (Satureja Hortensis L.) Essential Oil and Its Constituents. Journal of Horticultural Science and Biotechnology, 64: 205–210.
  10. Du E, Gan L, Li Z, Wang W, Liu D, Guo Y, 2015. In Vitro Antibacterial Activity of Thymol and Carvacrol and Their Effects on Broiler Chickens Challenged with Clostridium Perfringes. Journal of Animal Science and Biotechnology, 6:58. doi: 10.1186/s40104-015-0055-7. Embuscado ME, 2015. Spices and herbs: Natural sources of antioxidants- a mini review. Journal of Functional Foods, 18: 811-819. Ereifej K