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Journals / Gazi University Journal of Science / 2021 / Cilt: 34 - Sayı: 2

Optimization of Endo-Pectinase and Pectin Lyase Production from Wheat Bran by Bacillus pumilus using Response Surface Methodology

Özlem TEPE
Arzu Yadigar DURSUN
Pages
335–353
Publication Date
2021-06-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, these studies revealed that wheat bran can be used for the production of endo-pectinase and pectin lyase enzymes based on high enzyme activities.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Wheat bran is one of the most used agricultural residues for enzyme synthesis.
📊
Key Findings & Data:According to the model design, the optimum conditions were 4.74% w/v wheat bran, 0.12% ammonium sulphate and 0.12% yeast extract.
💡
Conclusion & Impact:In conclusion, these studies revealed that wheat bran can be used for the production of endo-pectinase and pectin lyase enzymes based on high enzyme activities.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Endo-pectinase · Optimization · Pectin lyase

Abstract

Wheat bran is one of the most used agricultural residues for enzyme synthesis. It has a high organic content; thus, it causes environmental pollution. In this study, wheat bran was selected as a carbon source and it was supplemented with yeast extract and ammonium sulphate. Endopectinase and pectin lyase production by Bacillus pumilus was performed in a batch system. RSM was applied to examine the effects of the wheat bran, yeast extract and ammonium sulphate concentrations on the synthesis of enzymes and the dosages of these nutrients were optimized. According to the model design, the optimum conditions were 4.74% w/v wheat bran, 0.12% ammonium sulphate and 0.12% yeast extract. The high values of R2 and R2 adj indicated that the fitted model showed good agreement with the predicted and actual values. In conclusion, these studies revealed that wheat bran can be used for the production of endo-pectinase and pectin lyase enzymes based on high enzyme activities.

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Cite This Article

TEPE, Ö., DURSUN, A. Y. (2021). Optimization of Endo-Pectinase and Pectin Lyase Production from Wheat Bran by Bacillus pumilus using Response Surface Methodology. Gazi University Journal of Science, Cilt: 34 - Sayı: 2, 335–353. https://ajindex.com/articles/254348

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