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Journals / Communications Faculty of Sciences University of Ankara Series C Biology / 2015 / Cilt: 24 - Sayı: 1

DIFFERENT APPROACHES FOR BREAST CANCER: VOLTAGE GATED POTASSIUM CHANNELS AND MICRORNAS

Çağrı ÖNER
Ertuğrul ÇOLAK
Didem TURGUT COŞAN
Pages
1–17
Publication Date
2015-06-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our results showed an increase in miR-126/126* expressions after the channel inhibition of MCF-7 and MDA-MB-231 cell lines (P < 0.001). miR-126/126* expressions were increased using TEA, 4-AP and astemizole in both cell lines. miR-126/126* expressions were only increased through the use of margatoxin in MCF-7. miR-126/126* may interact with voltage-gated potassium channels.

📌 3 Key Takeaways

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Objective & Hypothesis:Öz Micro RNAs and voltage-gated potassium channels (VGPCs) both play critical roles in the development of cancer.
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Key Findings & Data:We aimed to reveal the diversity of miR-126/126*, which effects angiogenesis and vascular development through the inhibition of VGPCs.
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Conclusion & Impact:Our results showed an increase in miR-126/126* expressions after the channel inhibition of MCF-7 and MDA-MB-231 cell lines (P < 0.001). miR-126/126* expressions were increased using TEA, 4-AP and astemizole in both cell lines. miR-126/126* expressions were only increased through the use of margatoxin in MCF-7. miR-126/126* may interact with voltage-gated potassium channels.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Anahtar Kelimeler Breast Cancer · miR-126/126* · Voltage-Gated Potassium Channels

Özet

Öz Micro RNAs and voltage-gated potassium channels (VGPCs) both play critical roles in the development of cancer. We aimed to reveal the diversity of miR-126/126*, which effects angiogenesis and vascular development through the inhibition of VGPCs. In this study, potassium channel inhibitors, including tetraethylammonium (5mM), 4-aminopyridine (5mM), margatoxin (1µM) and astemizole (2µM), were applied to MCF-7 and MDA-MB-231 breast cancer cell lines. After totally isolating RNA from the cells, Real-Time Polymerase Chain Reaction was used in order to identify gene expressions. OneWay ANOVA was used for variation analyses, while Tukey HSD and Tamhane were used to assess whether multiple comparisons were statistically significant (P < 0.001). Our results showed an increase in miR-126/126* expressions after the channel inhibition of MCF-7 and MDA-MB-231 cell lines (P < 0.001). miR-126/126* expressions were increased using TEA, 4-AP and astemizole in both cell lines. miR-126/126* expressions were only increased through the use of margatoxin in MCF-7. miR-126/126* may interact with voltage-gated potassium channels. In our study, the inhibition of K channels using K channel blockers resulted in an increase of miR-126/126* expression. Therefore, our data suggested that there could be another perspective between K channels and non-coding RNAs in the development of breast cancer

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Cite This Article

ÖNER, Ç., ÇOLAK, E., COŞAN, D. T. (2015). DIFFERENT APPROACHES FOR BREAST CANCER: VOLTAGE GATED POTASSIUM CHANNELS AND MICRORNAS. Communications Faculty of Sciences University of Ankara Series C Biology, Cilt: 24 - Sayı: 1, 1–17. https://ajindex.com/articles/247898

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