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Journals / Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2018 / Cilt: 11 - Sayı: 2

Determination of β-Sitosterol in Alchemilla caucasica Buser by GC-MS Method

Esen SEZEN KARAOĞLAN
Bilal YILMAZ
Pages
149–157
Publication Date
2018-08-31
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Also, this method can be used for the determination of β-sitosterol in A. caucasica plant.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, it was aimed to develop a method for the determination of β-sitosterol by gas chromatography-mass spectrometry method in Alchemilla caucasica Buser.
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Key Findings & Data:Calibration linearity of the devoloped method was obtained at 1-100 μg/mL concentration for β-sitosterol.
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Conclusion & Impact:Also, this method can be used for the determination of β-sitosterol in A. caucasica plant.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Alchemilla caucasica · β-sitosterol · GC-MS

Abstract

In this study, it was aimed to develop a method for the determination of β-sitosterol by gas chromatography-mass spectrometry method in Alchemilla caucasica Buser. For β-sitosterol, selected ion monitoring and electron ionization modes were used. Calibration linearity of the devoloped method was obtained at 1-100 μg/mL concentration for β-sitosterol. The intra-day and inter-day precision value for β-sitosterol was less than 4.99%. The accuracy of the method was observed to be better than 4.60%. The detection and quantification limits for β-sitosterol were found 0.05 and 0.15 μg/mL, respectively. The amount of β-sitosterol in A. caucasica plant was found to be 22.6 μg/1.0 mg with percent recovery 2.26% for β-sitosterol. Also, this method can be used for the determination of β-sitosterol in A. caucasica plant.

Özet

Bu çalışmada Alchemilla caucasica bitkisinde β-sitosterol’ün gaz kromatografisi-kütle spektrometri yöntemiyle belirlenmesi için bir yöntem geliştirmesi amaçlandı. β-sitosterol için, seçilmiş iyon görüntüleme ve elektron iyonizasyon modları kullanıldı. Geliştirilen yöntemin doğrusal aralığı β-sitosterol için 1-100 μg/mL olarak elde edildi. β-sitosterol için gün-içi ve günler-arası kesinlik değerinin %4.99'dan daha düşüktü. Yöntemin doğruluğunun %4.60'dan daha iyi olduğu görüldü. Gözlenebilme ve miktar tayin sınırları β-sitosterol için sırasıyla 0.05 ve 0.15 μg/mL olarak bulundu. A. caucasica bitkisinde β-sitosterol miktarı 2.26%’lık geri kazanımıyla 22.6 μg/1.0 mg olarak bulundu. Öyle ki bu yöntem, A. caucasica bitkisinde β-sitosterol’ün tayini için de kullanılabilir.

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Cite This Article

KARAOĞLAN, E. S., YILMAZ, B. (2018). Determination of β-Sitosterol in Alchemilla caucasica Buser by GC-MS Method. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 11 - Sayı: 2, 149–157. https://ajindex.com/articles/234391

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References (10)

  1. Awad, A.B., Chan, K.C., Downie, A.C., Fink, C.S. 2000. Peanuts as a source of beta sitosterol, a sterol with anticancer properties. Nutrition and Cancer, 36, 238-241.
  2. Aviram, M. 2000. Review of human studies on oxidative damage and antioxidative damage and antioxidant protection related to cardiovascular disease. Free Radical Research, 33, 585-597.
  3. Davis, P.H. 1972. Flora of Turkey and the East Aegean Islands, Edinburgh University Press, 4, 88. Falchero, L., Coppa, M., Fossi, A., Lombardi, G., Ramella, D., Tava A. 2009. Essential oil composition of lady's mantle (Alchemilla xanthochlora Rothm.) growing wild in Alpine pastures. Natural Product Research, 23, 1367-1372.
  4. Filipek, J. 1992. Effect of Alchemilla xantochlora Water Extract on Lipid Peroxidation and Superoxide Anion Scavenging Activity. Pharmazie, 47, 717-718.
  5. Fraile, L., Crisci, E., Córdoba, L., Navarro, M.A., Osada, J., Montoya, M. 2012. Immunomodulatory properties of beta-sitosterol in pig immune responses. International Immunopharmacology, 13, 316-321. Guidance for Industry Bioanalytical Method Validation. 2001. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research. Rockville, MD. Ho, Y., Lee, Y.L., Hsu, K.Y. 1995. Determination of (+) -catechin in plasma by high-performance liquid chromatography using fluorescence detection. Journal of Chromatography B: Biomedical Sciences and Applications, 665, 383-389.
  6. Ibrahim, T.A. 2012. Chemical composition and biological activity of extracts from Salvia bicolor Desf. growing in Egypt. Molecules, 17, 11315-11334.
  7. Igwe, O.U., Okwu, D.E. 2013. GC-MS evaluation of bioactive compounds and antibacterial activity of the oil fraction from the seeds of Brachystegia eurycoma (HARMS). Asian Journal of Plant Science and Research, 3(2), 47-54.
  8. Jonadet, M., Meunier, M.T., Villie, F., Bastide, J.P., Lamaison, J.L. 1986. "Flavonoids extracted from Ribes nigrum L. and Alchemilla vulgaris L.: 1. In vitro inhibitory activities on elastase, trypsin and chymotrypsin. 2. Angioprotective activities compared in vivo. Journal of Pharmacology, 17, 21-27.
  9. Jonathan, G., Ohimain, E., Kigigha, L. 2007. Antagonistic effects of extracts of some Nigerian higher fungi against selected microorganisms. American-Eurasian Journal of Agricultural & Environmental Sciences, 2, 364-368.
  10. Kathirvel, A., Sujatha, V. 2016. Phytochemical studies, antioxidant activities and identification of active compounds using GC-MS of Dryopertis cochleota