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Journals / Turkish Journal of Medical Sciences / 2014 / Cilt: 44 - Sayı: 3

Immunohistochemical investigation of galectin-3 in the skin of mice applied with Origanum hypericifolium essential oil and irradiated with ultraviolet B

Pınar İLİ
Nazan KESKİN
Pages
417–421
Publication Date
2014-05-18
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However, there is a need to research these findings with further molecular analyses.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:To demonstrate galectin-3-immunoreactivity in the undiluted essential oil of Origanum hypericifolium when applied to the ultraviolet B (UVB) irradiated skin of mice.
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Key Findings & Data:Materials and methods: Female BALB/c mice were allocated to 4 groups, each comprising 6 mice (Group 1: control; Group 2: UVB irradiated control; Group 3: undiluted O. hypericifolium essential oil applied; Group 4: undiluted O. hypericifolium essential oil applied before UVB irradiation).
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Conclusion & Impact:However, there is a need to research these findings with further molecular analyses.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Origanum hypericifolium · skin · UVB

Abstract

To demonstrate galectin-3-immunoreactivity in the undiluted essential oil of Origanum hypericifolium when applied to the ultraviolet B (UVB) irradiated skin of mice. Materials and methods: Female BALB/c mice were allocated to 4 groups, each comprising 6 mice (Group 1: control; Group 2: UVB irradiated control; Group 3: undiluted O. hypericifolium essential oil applied; Group 4: undiluted O. hypericifolium essential oil applied before UVB irradiation). One week prior to UVB irradiation, the undiluted O. hypericifolium essential oil was applied to the shaved dorsal skin of mice 3 times a week. Subsequently, the mice were irradiated 3 times per week with UVB (week 1: 50 mJ/cm2, week 2: 70 mJ/cm2, and weeks 3 and 4: 80 mJ/cm2) for 4 weeks. At the end of this period, immunohistochemical staining for galectin-3 was performed on frozen sections of skin specimens, and then they were photographed. Results: Numerous galectin-3-immunoreactive cells, which were considered to be immune system cells, were observed in the dermis of Group 3. Conclusion: It is suggested that undiluted O. hypericifolium essential oil may cause an increase in the galectin-3-immunoreactive cells. However, there is a need to research these findings with further molecular analyses.

Özet

To demonstrate galectin-3-immunoreactivity in the undiluted essential oil of Origanum hypericifolium when applied to the ultraviolet B (UVB) irradiated skin of mice. Materials and methods: Female BALB/c mice were allocated to 4 groups, each comprising 6 mice (Group 1: control; Group 2: UVB irradiated control; Group 3: undiluted O. hypericifolium essential oil applied; Group 4: undiluted O. hypericifolium essential oil applied before UVB irradiation). One week prior to UVB irradiation, the undiluted O. hypericifolium essential oil was applied to the shaved dorsal skin of mice 3 times a week. Subsequently, the mice were irradiated 3 times per week with UVB (week 1: 50 mJ/cm2, week 2: 70 mJ/cm2, and weeks 3 and 4: 80 mJ/cm2) for 4 weeks. At the end of this period, immunohistochemical staining for galectin-3 was performed on frozen sections of skin specimens, and then they were photographed. Results: Numerous galectin-3-immunoreactive cells, which were considered to be immune system cells, were observed in the dermis of Group 3. Conclusion: It is suggested that undiluted O. hypericifolium essential oil may cause an increase in the galectin-3-immunoreactive cells. However, there is a need to research these findings with further molecular analyses.

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Cite This Article

İLİ, P., KESKİN, N. (2014). Immunohistochemical investigation of galectin-3 in the skin of mice applied with Origanum hypericifolium essential oil and irradiated with ultraviolet B. Turkish Journal of Medical Sciences, Cilt: 44 - Sayı: 3, 417–421. https://ajindex.com/articles/228093

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