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Journals / Turkish Journal of Medical Sciences / 2014 / Cilt: 44 - Sayı: 3

Protective effects of montelukast and Hypericum perforatum against intestinal ischemia-reperfusion injury in hamsters

Tarık OCAK
Arif DURAN
Gülzade ÖZYALVAÇLI
Zeynep OCAK
Pages
381–386
Publication Date
2014-05-18
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: This study demonstrated the preventive effects of montelukast and Hypericum perforatum on I/R-induced intestinal injury.

📌 3 Key Takeaways

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Objective & Hypothesis:To evaluate the effects of montelukast and Hypericum perforatum against ischemia/reperfusion (I/R)-induced intestinal damage.
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Key Findings & Data:Materials and methods: Twenty-eight hamsters were divided into 4 groups following midline abdominal laparotomy: control group (n = 7), I/R group (n = 7), montelukast and I/R (MIR) group (n = 7), and Hypericum perforatum and I/R (HPIR) group (n = 7).
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Conclusion & Impact:Conclusion: This study demonstrated the preventive effects of montelukast and Hypericum perforatum on I/R-induced intestinal injury.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Montelukast · Hypericum perforatum · intestinal ischemia-reperfusion injury

Abstract

To evaluate the effects of montelukast and Hypericum perforatum against ischemia/reperfusion (I/R)-induced intestinal damage. Materials and methods: Twenty-eight hamsters were divided into 4 groups following midline abdominal laparotomy: control group (n = 7), I/R group (n = 7), montelukast and I/R (MIR) group (n = 7), and Hypericum perforatum and I/R (HPIR) group (n = 7). After 60 min of ischemia through obstruction of the superior mesenteric artery, 24 h of reperfusion was maintained. Ten minutes prior to the reperfusion period, the MIR group received 7 mg/kg of intraperitoneal montelukast and the HPIR group received 7 mg/kg of intraperitoneal Hypericum perforatum. Malondialdehyde, glutathione, myeloperoxidase, and cardiotrophin-1 levels were measured from blood samples. A semiquantitative histological evaluation was performed. Results: Montelukast and Hypericum perforatum significantly reduced malondialdehyde levels and increased glutathione levels compared to the I/R group (P < 0.008). A statistically significant difference was also found between the I/R group and MIR and HPIR groups in terms of myeloperoxidase levels (P < 0.008). The MIR and HPIR groups showed increased cardiotrophin-1 levels compared to the control and I/R groups (P < 0.008 for all). The MIR and HPIR groups showed significantly lower histological scores compared to the I/R group (P = 0.03 and P = 0.007, respectively). Conclusion: This study demonstrated the preventive effects of montelukast and Hypericum perforatum on I/R-induced intestinal injury.

Özet

To evaluate the effects of montelukast and Hypericum perforatum against ischemia/reperfusion (I/R)-induced intestinal damage. Materials and methods: Twenty-eight hamsters were divided into 4 groups following midline abdominal laparotomy: control group (n = 7), I/R group (n = 7), montelukast and I/R (MIR) group (n = 7), and Hypericum perforatum and I/R (HPIR) group (n = 7). After 60 min of ischemia through obstruction of the superior mesenteric artery, 24 h of reperfusion was maintained. Ten minutes prior to the reperfusion period, the MIR group received 7 mg/kg of intraperitoneal montelukast and the HPIR group received 7 mg/kg of intraperitoneal Hypericum perforatum. Malondialdehyde, glutathione, myeloperoxidase, and cardiotrophin-1 levels were measured from blood samples. A semiquantitative histological evaluation was performed. Results: Montelukast and Hypericum perforatum significantly reduced malondialdehyde levels and increased glutathione levels compared to the I/R group (P < 0.008). A statistically significant difference was also found between the I/R group and MIR and HPIR groups in terms of myeloperoxidase levels (P < 0.008). The MIR and HPIR groups showed increased cardiotrophin-1 levels compared to the control and I/R groups (P < 0.008 for all). The MIR and HPIR groups showed significantly lower histological scores compared to the I/R group (P = 0.03 and P = 0.007, respectively). Conclusion: This study demonstrated the preventive effects of montelukast and Hypericum perforatum on I/R-induced intestinal injury.

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Cite This Article

OCAK, T., DURAN, A., ÖZYALVAÇLI, G., OCAK, Z. (2014). Protective effects of montelukast and Hypericum perforatum against intestinal ischemia-reperfusion injury in hamsters. Turkish Journal of Medical Sciences, Cilt: 44 - Sayı: 3, 381–386. https://ajindex.com/articles/228087

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References (13)

  1. Kong SE, Blennerhassett LR, Heel KA, McCauley RD, Hall JC. Ischaemia-reperfusion injury to the intestine. Aust N Z J Surg 1998; 68: 554–561.
  2. Cerqueira NF, Hussni CA, Yoshida WB. Pathophysiology of mesenteric ischemia/reperfusion: a review. Acta Cir Bras 2005; 20: 336–343.
  3. Belviranlı M, Okudan N, Gökbel H, Kıyıcı A, Öz M, Kumak A. Cytokeratin 18 and h-FABP levels in intestinal ischemia– reperfusion injury: role of coenzyme Q10. Turk J Med Sci 2013; 43: 6–11.
  4. Li C, Jackson RM. Reactive species mechanisms of cellular hypoxia-reoxygenation injury. Am J Physiol Cell Physiol 2002; 282: 227–241.
  5. Filho DW, Torres MA, Bordin AL, Crezcynski-Pasa TB, Boveris A. Spermatic cord torsion, reactive oxygen and nitrogen species and ischemia-reperfusion injury. Mol Aspects Med 2004; 25: 199–210.
  6. Kelly KJ, Williams WW Jr, Colvin RB, Meehan SM, Springer TA, Gutierrez-Ramos JC, Bonventre JV. Intercellular adhesion molecule-1-deficient mice are protected against ischemic renal injury. J Clin Invest 1996; 97: 1056–1063.
  7. Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52: 302–310.
  8. Carsin H, Bargues L, Stephanazzi J, Paris A, Aubert P, Le Bever H. Inflammatory reaction and infection in severe burns. Pathol Biol (Paris) 2002; 50: 93–101.
  9. Wallace JL, McKnight GW, Keenan CM, Byles NI, MacNaughton WK. Effects of leukotrienes on susceptibility of the rat stomach to damage and investigation of the mechanism of action. Gastroenterology 1990; 98: 1178–1186.
  10. Sener G, Kabasakal L, Cetinel S, Contuk G, Gedik N, Yegen B. Leukotriene receptor blocker montelukast protects against burn-induced oxidative injury of the skin and remote organs. Burns 2005; 31: 587–596.
  11. Ozturk N, Korkmaz S, Ozturk Y. Wound healing activity of St. John’s wort (Hypericum perforatum L.) on chicken embryonic fibroblasts. J Ethopharmacol 2007; 111: 33–39.
  12. Di Paola R, Mazzon E, Muià C, Crisafulli C, Genovese T, Di Bella P, Esposito E, Menegazzi M, Meli R, Suzuki H et al. Protective effect of Hypericum perforatum in zymosan-induced multiple organ dysfunction syndrome: relationship to its inhibitory effect on nitric oxide production and its peroxynitrite scavenging activity. Nitric Oxide 2007; 16: 118–130.
  13. Paterniti I, Briguglio E, Mazzon E, Galuppo M, Oteri G, Cordasco G, Cuzzocrea S. Effects of Hypericum perforatum, in a rodent model of periodontitis. BMC Complement Altern Med 2010; 10: 73. Mukherjee PK, Verpoorte R, Suresh B. Evaluation of invivo wound healing activity of Hypericum patulum (family: