SavedAdd your journal

Journals / Turkish Journal of Medical Sciences / 2014 / Cilt: 44 - Sayı: 3

Chronic moderate alcohol consumption induces iNOS expression in the penis: An immunohistochemical study

Süheyla GONCA
Yusufhan YAZIR
Semil Selcan GÖÇMEZ
Ekim Nur DALÇIK
Tijen UTKAN
Hakkı DALÇIK
Pages
375–380
Publication Date
2014-05-18
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These abnormalities are also related to the defense mechanism against morphological damage.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:To investigate the effect of moderate alcohol consumption on metabolic alterations, inducible nitric oxide synthase (iNOS), immunohistochemical distribution, and morphological damage to penile erectile tissue in rats.
📊
Key Findings & Data:Materials and methods: Male Wistar albino rats were divided into 2 groups.
💡
Conclusion & Impact:These abnormalities are also related to the defense mechanism against morphological damage.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Chronic moderate alcohol consumption · iNOS · immunohistochemistry

Abstract

To investigate the effect of moderate alcohol consumption on metabolic alterations, inducible nitric oxide synthase (iNOS), immunohistochemical distribution, and morphological damage to penile erectile tissue in rats. Materials and methods: Male Wistar albino rats were divided into 2 groups. Group 1 rats (control group, n = 8) received tap water ad libitum, and group 2 rats (n = 8) were fed with 20% ethanol. Increasing levels of alcohol were given to the rats over 12 weeks. Immunohistochemistry was then performed using the avidin-biotin-peroxidase technique on 5-um thickness tissue sections. Stained sections were examined by imaging microscope. Results: Alcohol consumption resulted in a significant increase in iNOS immunoreactivity in the penile erectile tissue. Increased iNOS expression was determined in the tunica albuginea, cavernosal smooth muscle cells, trabeculae of connective tissue, arterioles, and the urethral epithelium. Moreover, chronic alcohol consumption resulted in decreasing serum testosterone and high density lipoprotein (HDL) levels with increasing cholesterol and triglyceride levels. Conclusion: Chronic moderate alcohol consumption can affect penile erectile tissue by increasing iNOS immunoreactivity and induce histopathological damage such as penile fibrosis. These abnormalities are also related to the defense mechanism against morphological damage.

Özet

To investigate the effect of moderate alcohol consumption on metabolic alterations, inducible nitric oxide synthase (iNOS), immunohistochemical distribution, and morphological damage to penile erectile tissue in rats. Materials and methods: Male Wistar albino rats were divided into 2 groups. Group 1 rats (control group, n = 8) received tap water ad libitum, and group 2 rats (n = 8) were fed with 20% ethanol. Increasing levels of alcohol were given to the rats over 12 weeks. Immunohistochemistry was then performed using the avidin-biotin-peroxidase technique on 5-um thickness tissue sections. Stained sections were examined by imaging microscope. Results: Alcohol consumption resulted in a significant increase in iNOS immunoreactivity in the penile erectile tissue. Increased iNOS expression was determined in the tunica albuginea, cavernosal smooth muscle cells, trabeculae of connective tissue, arterioles, and the urethral epithelium. Moreover, chronic alcohol consumption resulted in decreasing serum testosterone and high density lipoprotein (HDL) levels with increasing cholesterol and triglyceride levels. Conclusion: Chronic moderate alcohol consumption can affect penile erectile tissue by increasing iNOS immunoreactivity and induce histopathological damage such as penile fibrosis. These abnormalities are also related to the defense mechanism against morphological damage.

🔖

Cite This Article

GONCA, S., YAZIR, Y., GÖÇMEZ, S. S., DALÇIK, E. N., UTKAN, T., DALÇIK, H. (2014). Chronic moderate alcohol consumption induces iNOS expression in the penis: An immunohistochemical study. Turkish Journal of Medical Sciences, Cilt: 44 - Sayı: 3, 375–380. https://ajindex.com/articles/228086

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Zachary S. Overview: how is alcohol metabolized by the body? Alcohol Res Health 2006; 29: 245–254.
  2. Bennion LJ, Li TK. Alcohol metabolism in American Indians and whites. Lack of racial differences in metabolic rate and liver alcohol dehydrogenase. N Engl J Med 1976; 294: 9–13.
  3. Kopun M, Propping P. The kinetics of ethanol absorption and elimination in twins and supplementary repetitive experiments in singleton subjects. Eur J Clin Pharmacol 1977; 11: 337–344.
  4. Kurban S, Mehmetoğlu İ. The effect of alcohol on total antioxidant activity and nitric oxide levels in the sera and brains of rats. Turk J Med Sci 2008; 38: 199–204.
  5. Yağmurca M, Uçar M, Fadılloğlu E, Erdoğan H, Öztürk F. The effects of nitric oxide on rat stomach injury ınduced by acetylsalicylic acid. Turk J Med Sci 2009; 39: 13–19.
  6. Vardı N, Öztürk F, Fadıllıoğlu E, Otlu A, Yağmurca M. Histological changes in the rat thoracic aorta after chronic nitric oxide synthase inhibition. Turk J Med Sci 2003; 33: 141– 147. Bush PA. Nitric oxide is a potent relaxant of human and rabbit corpus cavernosum. J Urol 1992; 147: 1650–1655.
  7. Aktuğ H, Çetintaş VB, Kosova B, Oltulu F, Demiray ŞB, Çavuşoğlu T, Akarca SO, Yavaşoğlu A. Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats. Turk J Med Sci 2012; 42: 830–838.
  8. Gökçe MF, Bağırıcı F, Demir Ş, Bostancı MÖ, Güven A. The effect of neuronal nitric oxide synthase inhibitor 7-nitroindazole on the cell death induced by zinc administration in the brain of rats. Turk J Med Sci 2009; 39: 197–202. Burnett AL. The role of nitric oxide in erectile dysfunction: implications for medical therapy. J Clin Hypertens (Greenwich) 2006; 8: 53–62.
  9. Ignarro LJ, Bush PA, Buga GM, Wood KS, Fukuto JM, Rajfer J. Nitric oxide and cyclic GMP formation upon electrical field stimulation cause relaxation of corpus cavernosum smooth muscle. Biochem Biophys Commun 1990; 170: 830–843.
  10. Yoneyama T, Ohkawa S, Watanabe T, Odamaki M, Kumagai H, Kimura M, Hishida A. The contribution of nitric oxide to renal vascular wall thickening in rats with L-NAME-induced hypertension. Virchows Arch 1998; 433: 549–555.
  11. Utkan T, Sarioglu Y, Yazir Y. Changes in the neurogenic and endothelium-dependent relaxant responses of rabbit corpus cavernosum smooth muscle after cavernous nerve neurotomy. Methods Find Exp Clin Pharmacol 2010; 3: 151 –156. Tirapelli CR, Fukada SY, Yogi A, Chignalia AZ, Tostes RC, Bonaventura D, Lanchote VL, Cunha