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Journals / Gülhane Tıp Dergisi / 2010 / Cilt: 52 - Sayı: 2

Enterococcus faecalis'in PMNL fonksiyonları üzerine etkisi: Bir in vitro çalışma

The effects of Enterococcus faecalis on PMNL functions: An in vitro study

Elif Aybala OKTAY
Uğur MUŞABAK
Güneş ŞAHİNKESEN
Veli ASLANALP
Abdullah KILIÇ
Pages
121–126
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, increased PMNL functions by mononuclear cells may have possible contributions to tissue damage in the endodontic infections with E. faecalis.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to evaluate the effects of E. faecalis on chemotactic and oxidative burst activity of polimorphonuclear leukocytes (PMNLs) using transwell system and flow cytometry.
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Key Findings & Data:Chemotactic and oxidative burst activity were significantly higher in PMNL cultures with E. faecalis culture supernatants alone or combined with stimulated mononuclear cell culture supernatants than those of PMNL cultures without E. faecalis and stimulated mononuclear cell culture supernatants.
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Conclusion & Impact:In conclusion, increased PMNL functions by mononuclear cells may have possible contributions to tissue damage in the endodontic infections with E. faecalis.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to evaluate the effects of E. faecalis on chemotactic and oxidative burst activity of polimorphonuclear leukocytes (PMNLs) using transwell system and flow cytometry. PMNL cultures of six healthy donors were incubated with or without E. faecalis culture supernatants in order to determine the effects of this microorganism on PMNL functions. In addition, the effects of soluble factors derived from active mononuclear cells on the PMNL functions against E. faecalis were tested in culture conditions. Chemotactic and oxidative burst activity were significantly higher in PMNL cultures with E. faecalis culture supernatants alone or combined with stimulated mononuclear cell culture supernatants than those of PMNL cultures without E. faecalis and stimulated mononuclear cell culture supernatants. Oxidative burst activity in PMNL cultures combined with E. faecalis and stimulated mononuclear cell culture supernatants were also higher than those of PMNL cultures with E. faecalis culture supernatants alone. In conclusion, increased PMNL functions by mononuclear cells may have possible contributions to tissue damage in the endodontic infections with E. faecalis. Additionally, soluble factors produced by active mononuclear cells may indirectly contribute to this process.

Özet

Bu çalışmanın amacı, kuyucuklu sistem ve akım sitometri kullanarak E. faecalis'in polimorfonükleer lökositlerin (PMNL) kemotaktik ve oksidatif patlama aktiviteleri üzerine olan etkilerini değerlendirmekti. Bu mikroorganizmanın PMNL fonksiyonları üzerine etkilerini tespit etmek için, altı sağlıklı vericiden hazırlanan PMNL kültürleri E. faecalis kültür süpernatanı ilave edilerek veya ilave edilmeden inkübe edildi. İlave olarak, aktive mononükleer hücrelerden kaynaklanan çözünür faktörlerin E. faecalis'e karşı PMNL fonksiyonları üzerine etkileri kültür koşullarında test edildi. Sadece E. faecalis kültür süpernatanı ile hazırlanan veya beraberinde uyarılmış MNH kültür süpernatanları içeren PMNL kültürlerinde kemotaktik ve oksidatif patlama aktiviteleri, E. faecalis ve uyarılmış mononükleer hücre kültür süpernatanları içermeyen PMNL kültürlerinden daha yüksekti. E. faecalis kültür süpernatanı ve beraberinde uyarılmış MNH kültür süpernatanı içeren PMNL kültürlerinde oksidatif patlama aktivitesi, sadece E. faecalis kültür süpernatanı içeren PMNL kültürlerinden de yüksekti. Sonuç olarak, E. faecalis nedenli endodontik enfeksiyonlarda artmış PMNL fonksiyonları doku hasarına katkıda bulunabilir. Ayrıca, aktive mononükleer hücrelerden üretilen çözünür faktörler dolaylı olarak bu sürece katkı sağlayabilir.

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Cite This Article

OKTAY, E. A., MUŞABAK, U., ŞAHİNKESEN, G., ASLANALP, V., KILIÇ, A. (2010). Enterococcus faecalis'in PMNL fonksiyonları üzerine etkisi: Bir in vitro çalışma. Gülhane Tıp Dergisi, Cilt: 52 - Sayı: 2, 121–126. https://ajindex.com/articles/226124

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