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Journals / Genel Tıp Dergisi / 2004 / Cilt: 14 - Sayı: 4

Phagocyting labeling of leukocytes with Tc-99m PLA (poly l-lactic acid) microspheres

Fagositik aktivasyon yoluyla lökosit işaretlemede Tc-99m poli (l-laktik) asit mikrokürelerinin kullanımı

İrfan PEKSOY
Betül ARICA
Tayan Meral ERCAN
H. Süheyla KAŞ
Mürvet TUNCEL
Burçin ŞENER
Ayşe AŞANSÜ
Işıl S. ÜNSAL
Ali Kemal OĞUZ
Pages
125–132
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Our results indicated the potential value of leukocyte labeling with Tc-99m PLA microspheres.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of this study was to prepare Tc-99m poly (l-lactic) acid (PLA) particles for phagocyting engulfment by leucocytes and determine optimization conditions.
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Key Findings & Data:Methods: PLA microspheres (1-5 µm) were prepared from PLA polymeres (MW=52.000) by solvent evaporation from methylene chloride.
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Conclusion & Impact:Conclusion: Our results indicated the potential value of leukocyte labeling with Tc-99m PLA microspheres.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Lökositler · Enflamasyon · Radyofarmasötikler

Abstract

Objective: The aim of this study was to prepare Tc-99m poly (l-lactic) acid (PLA) particles for phagocyting engulfment by leucocytes and determine optimization conditions. Methods: PLA microspheres (1-5 µm) were prepared from PLA polymeres (MW=52.000) by solvent evaporation from methylene chloride. Then, they were labeled with Tc-99m by stannous chloride reduction at pH 3 with an efficiency of >95% and a stability of >70% at 24 hours. 50 mL heparinized blood was obtained from normal adult volunteers. The precipitate was removed and the sediment was washed with 0.9 NaCl and then centrifuged at 600 rpm for 5 minutes. 50 mCi Tc-99m-PLA particles in 1 mL was added to precipitated leukocytes and rotated in mechanical shaker in incubation at 37oC for 1 hour. Tc-99m-PLA labeled leukocytes were separated by centrifugation, washed and suspended in platelet poor plasma. Results: Leukocytes were labeled with an efficiency of about 70%. The engulfment of PLA particles by monocytes and the integrity of cell membrane afterwards were demonstrated by electron microscopy. Chemotactic index was >2. The viability of leukocytes was >90%, determined with trypan blue. Conclusion: Our results indicated the potential value of leukocyte labeling with Tc-99m PLA microspheres. This may be especially useful in demonstrating chronic infection, because of the preference of monocytes in labeling.

Özet

Amaç: Bu çalışmanın amacı Tc-99m poli (l-laktik) asit (PLA) mikrokürelerini lökositleri fagositik yolla işaretleyebilmek için hazırlamak ve gerekli optimum şartları belirlemektir. Yöntem: PLA polimerlerinden (MA:52.000 D) çözücü buharlaştırma yöntemi ile PLA mikroküreleri (1-5 µm) hazırlandı. Daha sonra pH 3’te kalay klorürle Tc-99m ile işaretlendi, işaretleme verimi % 95’in ve stabilitesi ise 24 saat içinde % 70’in üzerinde bulundu. 50 ml heparinli kan gönüllü sağlıklı erişkin insanlardan alınarak oda sıcaklığında sedimantasyona bırakıldı ve lökositten zengin plazma elde edildi. 1 mL’de hazırlanan 50 mCi Tc-99m-PLA mikroküresi lökosit çökeleğine ilave edilip rotator bir mekanik sallayıcıda 37oC’de 1. saat inkubasyona bırakıldı. Tc-99m PLA ile işaretlenen lökositler santrifüj ile süspansiyondan ayrıldı, yıkandı ve trombositten fakir plazma ile süspansiyon yapıldı. Bulgular: Lökositlerin işaretlenme verimi yaklaşık % 70 bulundu. Monositlerin PLA mikrokürelerini fagosite ettiği elektron mikroskobunda gösterildi. Lökositlerin kemotaktik indeksi 2’nin üzerinde bulundu. Lökositlerin canlılığı Tripan mavisi ile değerlendirildi ve % 90’ın üzerinde bulundu. Sonuç: Tc-99m-PLA mikroküreleri ile lökosit işaretlemenin potansiyel değeri olduğu görülmektedir. Monositlerin daha belirgin işaretlenmesi nedeniyle özellikle kronik inflamasyonda kullanılabilir.

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Cite This Article

PEKSOY, İ., ARICA, B., ERCAN, T. M., KAŞ, H. S., TUNCEL, M., ŞENER, B., AŞANSÜ, A., ÜNSAL, I. S., OĞUZ, A. K. (2004). Phagocyting labeling of leukocytes with Tc-99m PLA (poly l-lactic acid) microspheres. Genel Tıp Dergisi, Cilt: 14 - Sayı: 4, 125–132. https://ajindex.com/articles/224997

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