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Journals / Genel Tıp Dergisi / 2003 / Cilt: 13 - Sayı: 3

The effects of İNOS inhibitors and antioxidants on kidney pathology and function in sepsis

Sepsiste indüklenebilen nitrik oksit sentaz inhibitörü (iNOS) ve antioksidanların böbrek hasarı ve fonksiyonlarına etkileri

Murat GÖLCÜK
Şükrü TOPRAK
MUSTAFA ŞAHİN
Hatice PAŞAOĞLU
Pages
95–103
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Aminoguanidin and pentoxifillin may be added to treatment protocols in sepsis in order to prevent kidney tissue damage and dysfunction both of which (at least partially) caused by elevated NO levels.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The aim of this study is to investigate the effects of pentoxyfillin, L-arginin and aminoguanidin on plasma malondialdehyde (MDA) and nitric oxide (NO) levels, and to determine the relations between MDA and NO levels on kidney pathology and functions.
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Key Findings & Data:The rats were divided into 6 groups, each containing 10 subjects.
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Conclusion & Impact:Conclusion: Aminoguanidin and pentoxifillin may be added to treatment protocols in sepsis in order to prevent kidney tissue damage and dysfunction both of which (at least partially) caused by elevated NO levels.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Modeller · teorik · Pentoksifilin

Abstract

Objective: The aim of this study is to investigate the effects of pentoxyfillin, L-arginin and aminoguanidin on plasma malondialdehyde (MDA) and nitric oxide (NO) levels, and to determine the relations between MDA and NO levels on kidney pathology and functions. Methods: Sixty Wistar Albino rats were used. The rats were divided into 6 groups, each containing 10 subjects. Sepsis was induced by cecal ligation and puncture (CLP) method. Group I: Sham group, Group II: CLP (sepsis), Group III: CLP + 10 mg/kg L-arginin administration, Group IV: CLP + 15 mg/kg aminoguanidin administration, Group V: CLP + L-arginin + aminoguanidin (as groups III and IV) Group VI: CLP+15 mg/kg/day pentoxyfillin. Blood samples were taken for the determination of NO, MDA, leukocyte counts, BUN, creatinine and as well as 1 g kidney tissue sample was obtained for determination of tissue damage. Results: Leukocyte count increased in CLP induced groups significantly. While NO levels were significantly higher in sepsis and L-arginin groups, the levels in aminoguanidin and aminoguanidin + L-arginin groups were similar to Sham group. BUN, creatinine levels were significantly higher in CLP applied groups thari in sham group, while values in aminoguanidin and aminoguanidin + L-arginin showed a significant improvement compared to sepsis and L-arginin groups. Renal tissue damage in sepsis and L-arginin groups were more severe than the other groups. MDA levels in CLP induced groups were found to be higher than the sham group. Conclusion: Aminoguanidin and pentoxifillin may be added to treatment protocols in sepsis in order to prevent kidney tissue damage and dysfunction both of which (at least partially) caused by elevated NO levels.

Özet

Amaç: Çalışmanın amacı deneysel sepsis modelinde pentoksifilin, L-arginin ve aminoguanidinin plazma nitrik oksid (NO) ve malondialdehit (MDA) düzeylerine etkisini belirlemek, NO 've MDA düzeyleri ile böbrek hasarı ve böbrek fonksiyonları arasındaki ilişkiyi incelemektir. Yöntem: Wistär albino cinsi 60 rat kullanıldı. Hatlar 10'arlı 6 gruba ayrıldı. Ratlarda çekum ligasyon-perforasyon (CLP) yöntemi ile sepsis geliştirildi. Ratlar, Grup I: Sham işlemi, Grup II: CLP (sepsis), Grup III: CLP + 10 mg/kg L-arginin , Grup IV: CLP +15 mg/kg aminoguanidin, Grup V: CLP + L-arginin + aminoguanidin (aynı dozlar), Grup Vl: CLP +15 mg/kg pentoksifilin şeklinde gruplara ayrıldı, işlemden 24 saat sonra ratlardan NO, MDA, lökosit, BUN, kreatinin tayini için kan örnekleri ve doku hasarını belirlemek için 1 g böbrek doku örnekleri alındı. Bulgular: Lökosit sayısı CLP uygulanan gruplarda anlamlı olarak arttı. NO düzeyleri sepsis grubu ve L-arginin grubunda anlamlı olarak yükselirken, aminoguanidin ve aminoguanidin + L-arginin gruplarında sham grubuna benzer bulundu. CLP uygulanan gruplarda BUN, kreatinin, sham grubuna göre anlamlı olarak yüksekti, ancak pentoksifilin, aminoguanidin ve aminoguanidin + L-arginin gruplarının değerleri sepsis ve L-arginin gruplarına göre anlamlı olarak düzelme gösterdiler. Sepsis ve L-arginin gruplarındaki MDA düzeyleri sham grubuna göre anlamlı olarak yüksek bulundu. Sonuç: Sepsiste artan MDA ve NO'nun neden olduğu böbrek hasarını ve böbrek fonksiyonlarmdaki bozulmayı önlemek amacıyla tedavi protokollerine pentoksifilin ve aminoguanidin eklenebilir.

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Cite This Article

GÖLCÜK, M., TOPRAK, Ş., ŞAHİN, M., PAŞAOĞLU, H. (2003). The effects of İNOS inhibitors and antioxidants on kidney pathology and function in sepsis. Genel Tıp Dergisi, Cilt: 13 - Sayı: 3, 95–103. https://ajindex.com/articles/224984

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