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Journals / Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi / 2020 / Cilt: 37 - Sayı: 1

Determination of some viruses by serological and molecular techniques in potato production areas in Tokat Province

Tokat ili patates üretim alanlarında bazı virüslerin serolojik ve moleküler yöntemlerle belirlenmesi

Şerife TOPKAYA
Pages
53–59
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The best of our knowledge, AMV was first determined on potato in Tokat province.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this research, it was aimed to determine existence of some viruses, causes problem, by serological and molecular techniques in potato production in Tokat province was carried out by serological and molecular tools.
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Key Findings & Data:The results revealed that 54 % of samples were infected with PVY, PLRV, and AMV.
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Conclusion & Impact:The best of our knowledge, AMV was first determined on potato in Tokat province.
🔬Design:Survey & Empirical Study
📍Scope:Comprehensive Sample
⚙️Variables:Tokat · Patates · Patates Y virüsü

Abstract

In this research, it was aimed to determine existence of some viruses, causes problem, by serological and molecular techniques in potato production in Tokat province was carried out by serological and molecular tools. For this purpose, the survey was conducted to collect samples in potato production areas in Başçiftlik, Niksar, and Artova districts of Tokat province. DAS-ELISA test was performed to determine the prevalence of Potato virus Y (PVY), Potato leaf roll virus (PLRV) and Alfalfa mosaic virus (AMV). All samples were also tested by RT-PCR using specific primers to confirm the results DAS-ELISA. The results revealed that 54 % of samples were infected with PVY, PLRV, and AMV. PVY was the most common virus in the survey areas with the ratio of 50 %, followed by PLRV (5.5%) and AMV (1.38%) in all tested samples, respectively. Mixed infections also were detected within collected plants. The mixed infections PVY+PLRV was identified in four samples while each of mixed infection with PLRV+AMV, PVY+AMV and PVY+PLRV was represented with one isolate. RT-PCR amplified the expected PCR products for viruses. The best of our knowledge, AMV was first determined on potato in Tokat province.

Özet

Bu araştırmada, Tokat ili patates üretim alanlarında sorun olan viral etmenlerin varlığının serolojik ve moleküler olarak belirlenmesi amaçlanmıştır. Bu amaç kapsamında, Tokat ilinde patates yetiştiriciliğinin en çok yapıldığı Merkez, Niksar, Artova, Başçiftlik ilçelerine surveyler yapılmıştır. Surveyler esnasında toplanan örnekler Patates Y virus (Potato virus Y-PVY), Patates yaprak kırılma virüsü (Potato leaf roll virus-PLRV) ve Yonca mozaik virüsü (Alfalfa mosaic virus-AMV) etmenlerinin varlığını belirlemek için öncelikle serolojik olarak DAS-ELISA ile testlenmiştir. Bütün örnekler DAS-ELISA test sonuçlarını kontrol etmek amacıyla spesifik primerler kullanılarak RT-PCR ile de testlenmiştir. Çalışmalar sonucunda, bölgeden toplanan örneklerin %54’ünde bir veya birden fazla virüs enfeksiyonu tespit edilmiştir. Survey alanlarından toplanan örneklerde en yüksek oran ile %50 PVY tespit edilirken, %5.5 oranla PLRV ve %1.38 oranında AMV enfeksiyonu tespit edilmiştir. Toplanan örneklerde düşük oranlarda karışık enfeksiyonlarda görülmüş olup 4 bitkide PVY+PLRV tespit edilmiş iken, birer bitkide PLRV+AMV, PVY+AMV ve PVY+PLRV karışık enfeksiyonları belirlenmiştir. Çalışma konusu virüslere spesifik primerlerle yapılan RT-PCR çalışmaları sonucunda beklenilen büyüklükte PCR ürünleri elde edilmiştir. Yapılan bu çalışma ile Tokat ilinde patates alanlarında AMV’ nin varlığı ilk defa tespit edilmiştir.

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Cite This Article

TOPKAYA, Ş. (2020). Determination of some viruses by serological and molecular techniques in potato production areas in Tokat Province. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi, Cilt: 37 - Sayı: 1, 53–59. https://ajindex.com/articles/220537

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