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Journals / Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi / 2020 / Cilt: 37 - Sayı: 2

Multiple Shoot and Callus Formation in Different Explants of The Medicinally Important Peganum harmala L.

BURCU ÇETİN
Halil İbrahim KOÇAK
Pages
77–83
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The rooted plants were transferred to the soil with a success rate of 72%.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the micropropagation potential of the medicinally important Peganum harmala L. plant containing valuable metabolites has been examined.
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Key Findings & Data:Best callus growth were obtained in hypocotyl explants in Murashige and Skoog (MS) medium that contained 1 mg L-1 NAA and 1 mg L-1 BAP.
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Conclusion & Impact:The rooted plants were transferred to the soil with a success rate of 72%.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Peganum harmala L. · plant growth regulator · plant tissue culture

Abstract

In this study, the micropropagation potential of the medicinally important Peganum harmala L. plant containing valuable metabolites has been examined. For this purpose, hypocotyl and shoot tips isolated from P. harmala plants were applied different concentrations and combinations of plant growth regulators of benzyl amino purine (BAP), naphthalene acetic acid (NAA), and indole acetic acid (IAA). Best callus growth were obtained in hypocotyl explants in Murashige and Skoog (MS) medium that contained 1 mg L-1 NAA and 1 mg L-1 BAP. Best results concerning shoot count per explant (5.42±0.313 count) and shoot length (2.95±0.06 cm) were determined in MS medium which contained 1.5 mg L-1 BAP. IBA applications were performed in order to root the obtained shoots. The rooted plants were transferred to the soil with a success rate of 72%.

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Cite This Article

ÇETİN, B., KOÇAK, H. İ. (2020). Multiple Shoot and Callus Formation in Different Explants of The Medicinally Important Peganum harmala L.. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi, Cilt: 37 - Sayı: 2, 77–83. https://ajindex.com/articles/220533

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References (13)

  1. Aina OO, Quesenberry KH and Gallo M (2015). Culture vessel and auxin treatments affect in vitro rooting and ex vitro survival of six Arachis paraguariensis genotypes. Scientia Horticulturae, 183: 167-171.
  2. Al-Saikhan FI and Ansari MN (2016). Evaluation of the diuretic and urinary electrolyte effects of methanolic extract of Peganum harmala L. in Wistar albino rats. Saudi Journal of Biological Sciences, 23: 749-753.
  3. Asgarpanah J and Ramezanloo F (2012). Chemistry, pharmacology and medicinal properties of Peganum harmala L. African Journal of Pharmacy and Pharmacology, 6: 1573-1580.
  4. Baytop T (1999). Türkiye’de Bitkilerle Tedavi (Geçmişte ve Bugün). Nobel Tip Kitapevleri Ltd. Sti., 480 s., İstanbul,
  5. Turkey. Ebad FAS, El-Sadek MELSA and El-Kazzaz AA (2015). Micropropagation of four potato cultivars in vitro. Academia Journal of Agricultural Research, 3: 184-188.
  6. Diba K, Gerami Shoar M, Shabatkhori M, Khorshivand Z (2011) Antifungal activity of alcoholic extract of Peganum harmala seeds. Journal of Medicinal Plants Research. 5: 5550-5554.
  7. Ghimire BK, Kim HY, Seong ES, Yoo J H, Lee JH, Park SK, Kim SH and Chung IM (2018). Establishment of culturing conditions and assessment of antioxidant activity and somaclonal variation in the adventitious root suspension cultures of Oplopanax elatus Nakai. Acta Physiologiae Plantarum, 40: 51. Goel N, Singh N and Saini R (2009). Efficient in vitro multiplication of Syrian Rue (Peganum harmala L.) using 6-benzylaminopurine pre-conditioned seedling explants. Nature and Science, 7: 129-134.
  8. Jeevan P, Rena AE, Subramanian SS and Nelson R (2017). In vitro culture of Jatropha curcas L.–An important medicinal plant. Journal of Microbiology and Biotechnology Research, 3: 44-48.
  9. Kartal M, Altun ML and Kurucu S (2003). HPLC method for the analysis of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L. Journal of Pharmaceutical and Biomedical Analysis, 31: 263-269.
  10. Khan N, Chaudhary MF, Abbas AM, Khan SA, Nazir A and Shah GM (2017). Development of an efficient callus derived regeneration system for garlic (Allium sativum L.) from root explant. Journal of Plant Breeding and Agriculture, 1: 1-3.
  11. Kırıcı S, Kayıran Demirci S and Tokuz G (2018) Doğu Akdeniz Bölgesinde Üzerlik (Peganum harmala L.) bitkisinin tütsü olarak kullanımı. Lokman Hekim Dergisi, 8: 1-12.
  12. Kocaçalışkan İ (2017) Doku ve Hücre Kültürü Teknikleri. Nobel Yayın Dağıtım, 156 s., Ankara.
  13. Koyuncu O, Öztürk D, Potoğlu Erkara İ and Kaplan A (2008). Anatomical