SavedAdd your journal

Journals / Turkish Journal of Medical Sciences / 2014 / Cilt: 44 - Sayı: 6

Detection the frequency of PER-1 type extended-spectrum ß-lactamase-producing Acinetobacter baumannii clinical isolates in Turkey: a multicenter study

Detection Of The Frequency Of Per- Type STUDY
Pages
1041–1046
Publication Date
2014-06-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

However, the increased carbapenem resistance, together with multidrug resistance, has created a worrisome situation regarding this pathogen.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:b-Lactamases are an important resistance mechanism in Acinetobacter baumannii.
📊
Key Findings & Data:Pseudomonas extended-resistance (PER-1) type b-lactamase-producing strains have been reported from various geographic locations; however, PER-1 type b-lactamases from Turkish hospitals have not been investigated extensively.
💡
Conclusion & Impact:However, the increased carbapenem resistance, together with multidrug resistance, has created a worrisome situation regarding this pathogen.
🔬Design:Research Article
📍Scope:2008 Verileri
⚙️Variables:Acinetobacter baumannii · b-lactamases · PER-1

Abstract

b-Lactamases are an important resistance mechanism in Acinetobacter baumannii. Pseudomonas extended-resistance (PER-1) type b-lactamase-producing strains have been reported from various geographic locations; however, PER-1 type b-lactamases from Turkish hospitals have not been investigated extensively. The aim of this study was to determine the prevalence of PER-1 type b-lactamases in A. baumannii isolates in various regions of Turkey. Materials and methods: A total of 763 clinical A. baumannii isolates were collected from 9 university hospitals and 2 state hospitals between 2008 and 2011. Molecular amplification of the OXA-51 gene from the A. baumannii genome was performed in order to verify identification of the species. Real-time polymerase chain reaction was used to detect blaPER-1 genes. Results: PER-1 was detected in 24.6% of the isolates. The annual frequencies of the PER-1 enzyme were detected as 52.2%, 35.9%, and 8.3% in 2008, 2009, and 2010, respectively. PER-1 prevalence decreased gradually over time. The differences observed in PER-1 prevalence among the regions of Turkey were statistically significant (chi-square test; P < 0.001). Conclusion: These data demonstrate that the frequency of detection of PER-1 type b-lactamases in A. baumannii species has decreased in Turkey. However, the increased carbapenem resistance, together with multidrug resistance, has created a worrisome situation regarding this pathogen.

Özet

b-Lactamases are an important resistance mechanism in Acinetobacter baumannii. Pseudomonas extended-resistance (PER-1) type b-lactamase-producing strains have been reported from various geographic locations; however, PER-1 type b-lactamases from Turkish hospitals have not been investigated extensively. The aim of this study was to determine the prevalence of PER-1 type b-lactamases in A. baumannii isolates in various regions of Turkey. Materials and methods: A total of 763 clinical A. baumannii isolates were collected from 9 university hospitals and 2 state hospitals between 2008 and 2011. Molecular amplification of the OXA-51 gene from the A. baumannii genome was performed in order to verify identification of the species. Real-time polymerase chain reaction was used to detect blaPER-1 genes. Results: PER-1 was detected in 24.6% of the isolates. The annual frequencies of the PER-1 enzyme were detected as 52.2%, 35.9%, and 8.3% in 2008, 2009, and 2010, respectively. PER-1 prevalence decreased gradually over time. The differences observed in PER-1 prevalence among the regions of Turkey were statistically significant (chi-square test; P < 0.001). Conclusion: These data demonstrate that the frequency of detection of PER-1 type b-lactamases in A. baumannii species has decreased in Turkey. However, the increased carbapenem resistance, together with multidrug resistance, has created a worrisome situation regarding this pathogen.

🔖

Cite This Article

STUDY, D. O. T. F. O. P. T. (2014). Detection the frequency of PER-1 type extended-spectrum ß-lactamase-producing Acinetobacter baumannii clinical isolates in Turkey: a multicenter study. Turkish Journal of Medical Sciences, Cilt: 44 - Sayı: 6, 1041–1046. https://ajindex.com/articles/215202

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Bergogne-Berezin E. Resistance of Acinetobacter spp. to antimicrobials: overview of clinical resistance patterns and therapeutic problems. In: Bergogne-Berezin E, Joly-Guillou ML, Towner KJ, editors. Acinetobacter: Microbiology, Epidemiology, Infections, Management. Boca Raton, FL, USA: CRC Press; 1996. pp. 133–183.
  2. Munoz-Price LS, Weinstein RA. Acinetobacter infection. N Engl J Med 2008; 358: 1271–1281. Towner KJ. Molecular basis of antibiotic resistance in Acinetobacter spp. In: Gerisher U, editor. Acinetobacter Molecular Biology. Norfolk, UK: Caister Academic Press; 2008. pp. 322–343.
  3. Brown S, Young HK, Amyes SGB. Characterisation of OXA-51, a novel class D cephalosporinase found in genetically unrelated clinical strains of Acinetobacter baumannii from Argentina. Clin Microbiol Infect 2005; 11: 15–23.
  4. Nordman P, Ronco E, Naas T, Duport C, Michel Briand Y, Labia R. Characterization of a novel extended-spectrum β-lactamase from Pseudomonas aeruginosa. Antimicrob Agents Chemother 1993; 37: 962–969.
  5. Vahaboglu H, Ozturk R, Aygun G, Coskunkan F, Yaman A, Kaygusuz A, Leblebicioglu H, Balik I, Aydin K, Oktun M. Widespread of PER-1-type extended-spectrum β-lactamases among nosocomial Acinetobacter and Pseudomonas aeruginosa isolates in Turkey: a nationwide multicenter study. Antimicrob Agents Chemother 1997; 41: 2265–2269.
  6. Kolayli F, Gacar G, Karadenizli A, Sanic A, Vahaboglu H. PER- 1 is still widespread in Turkish hospitals among Pseudomonas aeruginosa and Acinetobacter spp. FEMS Microbiol Lett 2005; 249: 241–245.
  7. Erac B, Gulay Z. Molecular epidemiology of PER-1 extended spectrum β-lactamase among gram-negative bacteria isolated at a tertiary care hospital. Folia Microbiol 2007; 52: 535–541. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing, 17th Infor- mational Supplement. Wayne, PA, USA: Clinical and Labora- tory Standards Institute; 2007.
  8. Bogner PN, Killeen AA. Extraction of nucleic acids. In: Cole- man WB, Tsongalis GJ, editors. Molecular Diagnostics: For the Clinical Laboratorian. New York, NY, USA: Humana Press; 2007. pp. 25–30.
  9. Adams-Haduch JM, Paterson DL, Sidjabat HE, Pasculle AW, Potoski BA, Muto CA, Harrison LH, Doi Y. Genetic basis of multidrug resistance in Acinetobacter baumannii clinical iso- lates at a tertiary medical center in Pennsylvania. Antimicrob Agents Chemother 2008; 52: 3837–3843.
  10. Turner PJ, Greenhalgh JM; MYSTIC Study Group (Europe). The activity of meropenem and comparators a