AI Summary & Key Takeaways
AI InsightConclusion: Pure IgA given orally was identified to decrease intestinal damage and to prevent NEC in experimental NEC model.
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Abstract
Aim: Investigation of the protective effect of oral immung-lobulin(Ig) A on rat intestinum in experimental necrotising enterocolitis (NEC) model. Materials and methods: 40 newborn rats were divided into 4 groups each containing 10 rats. While control (C) group was fed by breast, the rats in necrotisinge enterocolitis (N), sham (S), and treatment (T) groups were settled into incubators at 36° C and 60 % humidity and fed, but not by breast. The rats in C group were fed by breast. The rats in N group were fed with Formula as soon as they were born. The rats in T group were fed with Formula and 600 mglkglday oral Ig A with 4-hour intervals. The rats in S group were fed with Formula and 0.1 ml/kg/day distilled water which is solvent of Ig. The rats in all groups were weighed and sacrified on fourth day. 2 cm intestinal segment from proximal of ileocaecal valve was used for histopathologic examination, another 10 cm intestinal segment for biochemical examination. After laparotomy, H&E was used for histopathological examination and apoptosis repressor with card Ab-1 cittfor immunohistochemical examination. Biochemical parameters such as my eloper oxidase (MPO), TNF-a, and IL-6 were evaluated. Results: The rate of mortality in N and S groups was significantly higher than T and C groups (P<0.05). Significant weight increase was identified in C group (P<0.05) There was significant decrease in T group in comparison of histopathologic values and apoptosis according to N and S groups (P<0.05). T group was significantly different in comparison of IL-6, TNF-a, and MPO according to S and N groups (P<0.05).There was no significant difference between T and C groups (P>0.05). Conclusion: Pure IgA given orally was identified to decrease intestinal damage and to prevent NEC in experimental NEC model.
Özet
Amaç: Deneysel/olarak oluşturulan nekrotizan enterokolit (NEK) modelinde, oral yoldan verilen immünglobülin A'nın sıçan barsağını koruyucu etkisini araştırmayı amaçladık. Gereç ve Yöntem: 40 yenidoğan sıçan 10'arlı gruplar halinde 4 gruba ayrıldı. Kontrol (K) grubu anne yanında bırakılırken, NEK (N), sham (S) ve tedavi (T) grubu anne sütü almadan, annesinden ayrı olarak 36°C de ve % 60'lık nemde beslenme ve bakım sağlanmak üzere inkübatöre yerleştirildi. K grubundaki sıçanlar anne sütü ile beslendi. N grubundaki denekler doğar doğmaz, hiç anne sütü almadan annelerinin yanından ayrılarak formula mama ile beslendi. T grubundaki sıçanlara hiç anne sütü almadan formula mamaya ilaveten 600 mglkglgün 6 doz halinde saf oral IgA verildi. S grubundaki deneklere hiç anne sütü almadan mamaya ilaveten 0.1 mllkglgün immunoglobulin çözücüsü olan distile su verildi. Tüm gruplardaki sıçanlar 4. gün tartılarak sakrifiye edildi. Laparatomi sonrası ile-oçekal valvin 1 cm proksimalinden 2 cm'lik barsak segmenti histopatolojik inceleme için, geri kalan 10 cm'lik segment biyokimyasal inceleme için çıkartıldı. H&E boyama ile histopatolojik, ARC (Apoptosis Repressor With CARD) Ab-1 apopitozis kiti kullanılarak immunohistokimyasal, doku Myeloperooksidaz (MPO), Tümör nekrozis faktör alfa (TNF-a), ve İnterlökin altı (IL-6) bakılarak biyokimyasal değerlendirme yapıldı. Bulgular: N ve S gruplarında mortalite oranı T ve K gruplarına göre anlamlı olarak yüksek bulundu (P 0.05). Sonuç: Oral yolla verilen saf lgA'nın deneysel NEK modelinde, intestinal hasan azalttığı ve NEK' ı önlediği görüldü.