Journals / Kocatepe Veteriner Dergisi / 2021 / Cilt: 14 - Sayı: 2

Investigation of the Effect of Vitamin C and Vitamin E on Serum Protein Fractions in Rats with Experimental Fluorosis

Deneysel Olarak Florozis Oluşturulan Ratlarda Vitamin C ve Vitamin E'nin Serum Protein Fraksiyonları Üzerindeki Etkisinin Araştırılması

Pages
262–267
Publication Date
2021-06-30
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In the groups in which vitamin C, vitamin E and combine combination of vitamin C and vitamin E were given for prevention and treatment, it was observed that these changing values approach to the control group.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of the present study was to investigate the effects of vitamins C (vit C) and E (vit E) on the serum protein fractions in rats with experimentally-induced chronic fluorosis.
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Key Findings & Data:Wistar-Albino rats (male) were used as material (8 groups, each containing 8 rats).
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Conclusion & Impact:In the groups in which vitamin C, vitamin E and combine combination of vitamin C and vitamin E were given for prevention and treatment, it was observed that these changing values approach to the control group.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Florozis · rat · serum proteinleri

Abstract

The aim of the present study was to investigate the effects of vitamins C (vit C) and E (vit E) on the serum protein fractions in rats with experimentally-induced chronic fluorosis. Wistar-Albino rats (male) were used as material (8 groups, each containing 8 rats). The experimental groups were control, protection groups (daily 150 ppm NaF containing drinking water for 16 weeks as ad-libitum, and, on alternate Vit C (100 mg/kg), Vit E (300 mg/kg) and Vit C+ Vit E (100 mg/kg + 300 mg/kg) for 16 weeks) therapy groups (daily 150 ppm NaF containing drinking water for 16 weeks as ad-libitum, then normal drinking water as ad-libitum, Vit C (100 mg/kg), Vit E (300 mg/kg) and Vit C + Vit E (100 mg/kg + 300 mg/kg) on alternate for 4 weeks). The end of experimental period, blood samples were collected and serums were obtained. Serum protein fractions in blood samples were determined with cellulose-acetate electrophoresis. Alpha-1 was significantly decreased and beta globulins increased in the experimental fluorosis group. In the groups in which vitamin C, vitamin E and combine combination of vitamin C and vitamin E were given for prevention and treatment, it was observed that these changing values approach to the control group.

Özet

Bu çalışma, deneysel olarak kronik florozis oluşturulan ratlarda vitamin C (vit C) ve E (vit E)'nin serum protein fraksiyonları üzerindeki etkilerini araştırmak amacıyla planlandı. Materyal olarak Wistar-Albino ratlar kullanıldı (Her biri 8 rat içeren, 8 grup). Deneme grupları; kontrol grubu, deneysel florozis grubu, koruma grupları (16 hafta boyunca, içme suyu içeren günlük 150 ppm NaF içeren içme suyu/ad-libitum ile birlikte vit C (100 mg / kg), vit E (300 mg / kg) ve vit C + vit E (100 mg/kg + 300 mg/kg)) ve tedavi grupları (16 hafta boyunca 150 ppm NaF içeren içme suyu/ad-libitum verildikten sonra tedavi amacıyla 4 hafta vit C (100 mg/kg), vit E (300 mg / kg) ve vit C + vit E (100 mg/kg + 300 mg/kg)) olarak oluşturuldu. Deneme sonunda kan örnekleri toplandı ve serumlar alındı. Bu örneklerde serum protein fraksiyonları selüloz-asetat elektroforezi ile belirlendi. Deneysel florozis grubunda total protein yüzde gram açısından, alfa-1’in önemli oranda azaldığı ve beta globülinlerin arttığı tespit edildi. Korunma ve tedavi amacıyla vitamin C, vitamin E ve vitamin C+vitamin E birlikte verildiği gruplarda, değişen bu değerlerin kontrol grubuna yaklaştığı görüldü.

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Cite This Article

DEDE, S., ÖNER, A. C., YÜKSEK, V., ÇETİN, S., USTA, A. (2021). Investigation of the Effect of Vitamin C and Vitamin E on Serum Protein Fractions in Rats with Experimental Fluorosis. Kocatepe Veteriner Dergisi, Cilt: 14 - Sayı: 2, 262–267. https://ajindex.com/articles/214708

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