Journals / Journal of the Entomological Research Society / 2011 / Cilt: 13 - Sayı: 1

Efficacy of Bt transgenic sugar beet lines expressing cry1Ab gene against Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae)

Pages
61–68
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In addition, the transgenic line S 37-3 was the most effective line among evaluated transgenic lines.

📌 3 Key Takeaways

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Objective & Hypothesis:The sugar beet, Beta vulgaris L., has some key pests in Iran that causes losses in crops.
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Key Findings & Data:Larval mortality, larval weight loss, and leaf weight loss as a damage index of this pest on 10 lines of transgenic and 2 non-transgenic sugar beet were studied.
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Conclusion & Impact:In addition, the transgenic line S 37-3 was the most effective line among evaluated transgenic lines.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:ölüm oranı · larva · şeker pancarı

Abstract

The sugar beet, Beta vulgaris L., has some key pests in Iran that causes losses in crops. One of these pests is Egyptian leaf worm, Spodoptera littoralis (Boisd). In addition to southern regions of Iran especially in Khuzestan province, this pest is distributed throughout the countries around the Mediterranean region. One of the most effective controlling measures against this pest is planting Bt transgenic sugar beet, expressing an insecticidal-protein gene from Bacillus thuringiensis subsp. kurstaki (Berliner). Larval mortality, larval weight loss, and leaf weight loss as a damage index of this pest on 10 lines of transgenic and 2 non-transgenic sugar beet were studied. In each treatment, 40 larvae of the Egyptian leaf worm (10 larvae/replication) were fed on the leaves of the transgenic lines in glass petri dishes. Larval mortality, weight of the alive larvae, and weight loss of the leaves were recorded after 3 and 6 days. ANOVA results revealed that there were significant differences between the treatments at the 1% probability level considering all evaluated factors. Therefore, mean comparisons were carried out using Duncan’s multiple range tests. Based on the results, all of the examined transgenic lines were more effective than non-transgenic line. In addition, the transgenic line S 37-3 was the most effective line among evaluated transgenic lines.

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Cite This Article

SEDIGHI, L., REZAPANAH, M., AGHDAM, H. R. (2011). Efficacy of Bt transgenic sugar beet lines expressing cry1Ab gene against Spodoptera littoralis Boisd. (Lepidoptera: Noctuidae). Journal of the Entomological Research Society, Cilt: 13 - Sayı: 1, 61–68. https://ajindex.com/articles/213248

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References (9)

  1. Abbot, W. S. A., 1925, Method of computing the effectiveness insecticide. Journal of Economic Entomology, 18: 265-276.
  2. Bayoumi, E., Balaña-Fouce, R., Sobeiha, K., Hussin, M. K., 1998, The biological activity of some chitin synthesis inhibitors against the cotton leafworm Spodoptera littoralis (Boisdual) (Lepidoptera:Noctuidae). Boletin de Sanidad Vegetal Plagas, 24: 499-506.
  3. Behdad, E., 1993. Pests of field crops in Iran. Yadboud Publication Center, Esfahan, Iran, 618. Ferry, N., Edwards MG, Gatehouse, J., Capell, T., Christou, P., Gatehouse A. M., 2006, Transgenic plants for insect pest control: a forward looking scientific perspective. Transgenic Research, 15:13-19. Gómez de Aizpurua, C., Arroyo, M., 1994, Principales noctuidos actuales de interés agrícola. Edifur, S. A. Madrid, España, 145. Harwood, J. D., Wallin W. G., Obrycki, J. J., 2005, Uptake of Bt endotoxins by nontarget herbivores and higher order arthropod predators: molecular evidence from a transgenic corn agroecosystem. Molecular Ecology, 14: 2815-2823.
  4. Hilder, V. A., Boulter, D., 1999, Genetic engineering of crop plants for insect resistance a critical review. Crop Protection, 18: 177-191.
  5. Hisano, H., Kimoto, Y., Hayakawa, H., Takeichi, J., Domae, T., Hashimoto, R. Abe, J., Asano, S., Kananzawa, A., Shimamato, Y., 2004, High frequency Agrobacterium- mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima. Plant Cell Reports, 22: 910-918.
  6. Jafari, M., Norouzi, P., Malboobi, M. A., Ghareyazie, B., Valizadeh, M., Mohammadi, S. A., Mousavi, M., 2009, Enhanced resistance to a lepidopteran pest in transgenic sugar beet plants expressing synthetic cry1Ab gene. Euphytica, 165: 333-344.
  7. Kimoto, Y., Shimamoto, Y., 2001, Difference in toxicity to larvae of cabbage armyworm between transgenic sugar beet lines with Cry1Ab and Cry1C. Proceeding of the Japanese Society of Sugar Beet Technologists, 43: 20-23.
  8. Macrae, T. C., Baur, M. E., Boethel, D.J., Fitzpatrick, B. J., Gao, A., Gamundi, J. C., Harisson, L. A., Kabuye, V. T., Mcpherson, R. M., Miklos, J.A., Paradise, M. S., Toedebusch, A. S., 2005, Laboratory and field evaluations of transgenic soybean exhibiting high-dose expression of a synthetic Bacillus thuringiensis cry1A gene for control of Lepidoptera. Journal of Economic Entomology, 98 (2): 577-587.
  9. Martins, T., 2000, Contribuição para o estudo da bioecologia de Spodoptera littoralis (B.) (Lepidoptera: Noctuidae) em São Miguel-