Vitamin C as an antioxidant: evaluation of its role on pulmonary contusion experimental model

Conclusion: The present results indicate that vitamin C administration attenuated the oxidative damage and morphological changes induced by pulmonary contusion in an experimental rat study.

📌 3 Key Takeaways

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Objective & Hypothesis:To analyze the protective activity of vitamin C on the lungs by assessing biochemical and histopathological analysis after performing an experimental isolated lung contusion model.

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Key Findings & Data:The rats were randomly separated into 4 groups.

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Conclusion & Impact:Conclusion: The present results indicate that vitamin C administration attenuated the oxidative damage and morphological changes induced by pulmonary contusion in an experimental rat study.

🔬Design:Modeling & Simulation

📍Scope:Comprehensive Sample

⚙️Variables:Vitamin C · antioxidant · lung injury

Abstract

To analyze the protective activity of vitamin C on the lungs by assessing biochemical and histopathological analysis after performing an experimental isolated lung contusion model. Materials and methods: Fifty-four male Sprague-Dawley male rats were used. The rats were randomly separated into 4 groups. Vitamin C (200 mg/kg) was injected intraperitoneally 30 min after trauma. Blood samples were obtained for myeloperoxidase (MPO), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities and malondialdehyde (MDA) levels. Blood gas analysis and bronchoalveolar lavage was performed. The lung tissue was also extracted for histopathological examination. Results: The lung contusion enhanced MDA, SOD, CAT, and MPO and diminished GSH-Px. Vitamin C administration after the pulmonary contusion was found to diminish the level of MDA and the activities of SOD, CAT, and MPO and to enhance the level of GSH-Px (P < 0.05). Contusion-induced disrupted gas analysis and leukocyte infiltration were both resolved by the vitamin C. Conclusion: The present results indicate that vitamin C administration attenuated the oxidative damage and morphological changes induced by pulmonary contusion in an experimental rat study.

Özet

To analyze the protective activity of vitamin C on the lungs by assessing biochemical and histopathological analysis after performing an experimental isolated lung contusion model. Materials and methods: Fifty-four male Sprague-Dawley male rats were used. The rats were randomly separated into 4 groups. Vitamin C (200 mg/kg) was injected intraperitoneally 30 min after trauma. Blood samples were obtained for myeloperoxidase (MPO), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities and malondialdehyde (MDA) levels. Blood gas analysis and bronchoalveolar lavage was performed. The lung tissue was also extracted for histopathological examination. Results: The lung contusion enhanced MDA, SOD, CAT, and MPO and diminished GSH-Px. Vitamin C administration after the pulmonary contusion was found to diminish the level of MDA and the activities of SOD, CAT, and MPO and to enhance the level of GSH-Px (P < 0.05). Contusion-induced disrupted gas analysis and leukocyte infiltration were both resolved by the vitamin C. Conclusion: The present results indicate that vitamin C administration attenuated the oxidative damage and morphological changes induced by pulmonary contusion in an experimental rat study.

Keywords

Vitamin C antioxidant lung injury pulmonary contusion

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Cite This Article

SIRMALI, R., GİNİŞ, Z., SIRMALI, M., SOLAK, O., ŞELİMAN, B., AĞAÇKIRAN, Y., DELİBAŞ, N. (2014). Vitamin C as an antioxidant: evaluation of its role on pulmonary contusion experimental model. Turkish Journal of Medical Sciences, Cilt: 44 - Sayı: 6, 905–913. https://ajindex.com/articles/212927

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