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Journals / Gülhane Tıp Dergisi / 2014 / Cilt: 56 - Sayı: 2

Evaluation of Culture Method and Real-Time PCR for Detection of Vaginal Group B Streptococcus Colonization in Pregnant at the Last Trimester

Son Trimester Gebelerde Vajinal Grup B Streptokok Kolonizasyonunun Saptanmasında Kültür Yöntemi ile Gerçek Zamanlı Polimeraz Zincir Reaksiyonu Yönteminin Etkinliklerinin Değerlendirilmesi

Samer ALWEDYAN
RAMAZAN GÜMRAL
ÜMİT GÖKTOLGA
FATİH ŞAHİNER
ORHAN BEDİR
AYLİN ÜSKÜDAR GÜÇLÜ
Abdullah KILIÇ
Ahmet Celal BAŞUSTAOĞLU
Pages
80–84
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this study, a statistically significant difference was found between these two methods by using Mac Nemar test (p<0.021).

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, it is aimed to evaluate effectiveness of the conventional culture method and PCR based molecular method in detecting the vaginal Group B Streptococcus (Streptococcus agalactiae) colonization during the third trimester of pregnancy.
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Key Findings & Data:One of the swabs was directly cultured by means of plating onto 5% sheep blood agar, while the other one was used for a real-time PCR assay.
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Conclusion & Impact:In this study, a statistically significant difference was found between these two methods by using Mac Nemar test (p<0.021).
🔬Design:Research Article
📍Scope:80 samples
⚙️Variables:Domain Parameters

Abstract

In this study, it is aimed to evaluate effectiveness of the conventional culture method and PCR based molecular method in detecting the vaginal Group B Streptococcus (Streptococcus agalactiae) colonization during the third trimester of pregnancy. Two vaginal samples were obtained simultaneously from each consenting pregnant women by using sterile swabs. One of the swabs was directly cultured by means of plating onto 5% sheep blood agar, while the other one was used for a real-time PCR assay. A total of 80 samples were collected in which 64 (80%) samples were found negative by both the culture and PCR methods, whereas remaining 16 (%20) were positive by at least one of the two methods; six samples were positive in both methods, nine were positive with PCR only, and one was positive with culture only. In this study, a statistically significant difference was found between these two methods by using Mac Nemar test (p<0.021).

Özet

Bu çalışmada konvansiyonel kültür yöntemi ile Polimeraz Zincir Reaksiyonu (PZR) temelli moleküler yöntemin son trimester gebelerde vajinal Grup B streptokok (Streptococcus agalactiae) kolonizasyonunu amaçlanmıştır. Steril svablar kullanılarak gönüllü gebe kadınların her birinden iki ayrı vajinal sürüntü örneği alındı. Örneklerden biri doğrudan %5 koyun kanlı agara ekilerek kültüre edilirken diğer örnek Gerçek Zamanlı PZR yöntemiyle test edildi. Toplam 80 örneğin dâhil edildiği çalışmada 6 örnekte her iki yöntem ile bir örnekte sadece kültür yöntemi ve 9 örnekte sadece PZR ile olmak üzere toplam 16 (%20) örnekte pozitiflik saptanırken 64 örnek (%80) yöntemlerin her ikisi ile de negatif olarak değerlendirildi. Bu çalışmada, kullanılan iki yöntem arasında Mc Nemar testi ile istatiksel olarak anlamlı fark (p<0.021) bulundu.

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Cite This Article

ALWEDYAN, S., GÜMRAL, R., GÖKTOLGA, Ü., ŞAHİNER, F., BEDİR, O., GÜÇLÜ, A. Ü., KILIÇ, A., BAŞUSTAOĞLU, A. C. (2014). Evaluation of Culture Method and Real-Time PCR for Detection of Vaginal Group B Streptococcus Colonization in Pregnant at the Last Trimester. Gülhane Tıp Dergisi, Cilt: 56 - Sayı: 2, 80–84. https://ajindex.com/articles/211788

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