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Journals / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2018 / Cilt: 24 - Sayı: 2

Brucella abortus L7/L12 ve BCSP31 Proteinlerini Birlikte Eksprese Eden Rekombinant Adenovirusların İmmunojenitesi

Immunogenicity of Recombinant Adenovirus Co-expressing the L7/L12 and BCSP31 Proteins of Brucella abortus

Zhong-Ren MA
Jun-Lin LIU
Kui-Zheng CAI
Yi-Zhong LIU
Guo-Zhen LIN
Pages
211–217
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

These results indicated that the recombinant Ad-LL/BP vaccine induced mainly cell-mediated immunity and partly humoral immunity, and provided moderately protection against B. abortus infection.

📌 3 Key Takeaways

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Objective & Hypothesis:Brucella poses a great threat to animal and human health, and vaccination is a good way of controlling the bacterium.
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Key Findings & Data:In this study, the immune response and protection ability of a recombinant adenovirus Ad-LL/BP containing L7/L12 and BCSP31 proteins of Brucella abortus (B. abortus) were evaluated in BALB/c mice model.
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Conclusion & Impact:These results indicated that the recombinant Ad-LL/BP vaccine induced mainly cell-mediated immunity and partly humoral immunity, and provided moderately protection against B. abortus infection.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Brucella poses a great threat to animal and human health, and vaccination is a good way of controlling the bacterium. In this study, the immune response and protection ability of a recombinant adenovirus Ad-LL/BP containing L7/L12 and BCSP31 proteins of Brucella abortus (B. abortus) were evaluated in BALB/c mice model. Firstly, Adenovirus vector Ad-CMV and the recombinant adenovirus Ad-LL/BP were amplified in HEK 293AD cells. The TCID50 values of Ad-LL/BP and Ad-CMV were 10−8.68/0.1 mL and 10−8.35/0.1 mL, respectively. Mice were inoculated with 100 TCID50/mouse of Ad-LL/BP or Ad-CMV. Vaccination of mice with Ad-LL/BP vaccine was able to elicit higher IgG, IgG1 and IgG2a antibody levels when compared with Ad-CMV and PBS control animals (P 0.05). Ad-LL/BP vaccine was able to reduce significantly the numbers of B. abortus in the spleens from the immunized mice. These results indicated that the recombinant Ad-LL/BP vaccine induced mainly cell-mediated immunity and partly humoral immunity, and provided moderately protection against B. abortus infection. Therefore, the vaccine could be further developed into a live-vector vaccine against B. abortus

Özet

Brucella insan ve hayvanlarda önemli bir tehdit olup, aşılama bakteriyi kontrol altında tutmak amacıyla iyi bir yöntem olabilir. Bu çalışmada, Brucella abortus (B. abortus) L7/L12 ve BCSP31 proteinlerini içeren rekombinant adenovirus Ad-LL/BP'nin immun yanıtı ve koruyuculuğu BALB/c farelerde araştırıldı. Adenovirus vektör Ad-CMV ve rekombinant adenovirus Ad-LL/BP, HEK 293AD hücrelerinde amplifiye edildi. Ad-LL/BP and Ad-CMV'nın TCID50 değerleri sırasıyla 10-8.68/0.1 mL ve 10-8.35/0.1 mL olarak tespit edildi. Fareler Ad-LL/BP veya Ad-CMV'nin 100 TCID50/fare ile inoküle edildi. Ad-LL/BP aşısı ile farelerin aşılanması, Ad-CMV ve PBS kontrol hayvanları ile karşılaştırıldığında daha yüksek IgG, IgG1 ve IgG2a antikor seviyelerinin olmasına neden oldu (P 0.05). Ad-LL/BP aşısı immunize edilmiş farelerin dalaklarında B. abortus miktarını anlamlı derecede azalttı. Bu sonuçlar rekombinant Ad-LL/BP aşısının çoğunlukla hücre-aracılı bağışıklığı ve kımen humoral bağışıklığı indüklediğini ve B. abortus enfeksiyonuna karşı orta seviyede koruyuculuk sağladığını göstermiştir. Bu nedenle aşının ileri çalışmalarda B. abortus'a karşı canlı-vektör aşı olarak geliştirilebileceği düşünülmektedir

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Cite This Article

MA, Z., LIU, J., CAI, K., LIU, Y., LIN, G. (2018). Brucella abortus L7/L12 ve BCSP31 Proteinlerini Birlikte Eksprese Eden Rekombinant Adenovirusların İmmunojenitesi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 24 - Sayı: 2, 211–217. https://ajindex.com/articles/211082

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References (1)

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