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Journals / Dokuz Eylül Üniversitesi Tıp Fakültesi Dergisi / 2003 / Cilt: 17 - Sayı: 1

Yeast strains isolated from intensive care unit patients and their antifungal susceptibilities

Yoğun bakım hastalarından soyutlanan maya türleri ve amfoterisin B ve flukonazole duyarlılıkları

Mine YÜCESOY
Serhat MAROL
Banu BİLİRGEN
Vildan ACARER
Pages
1–6
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

For this reason, special attention should be given when using fluconazole for the antifungal treatment.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: This study was designed to identify the yeast strains isolated from intensive care unit patients and to determine their antifungal susceptibilities.
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Key Findings & Data:Materials and methods: A total number of eighty six strains which were isolated from intensive care unit patients' clinical specimens between december 2001 and June 2002 were included in this study.
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Conclusion & Impact:For this reason, special attention should be given when using fluconazole for the antifungal treatment.
🔬Design:Research Article
📍Scope:2001 Verileri
⚙️Variables:Mayalar · Mikrobiyal duyarlılık testleri · İlaç direnci

Abstract

Objective: This study was designed to identify the yeast strains isolated from intensive care unit patients and to determine their antifungal susceptibilities. Materials and methods: A total number of eighty six strains which were isolated from intensive care unit patients' clinical specimens between december 2001 and June 2002 were included in this study. The strains were identified by germ tube test, morphology on cornmeal tween 80 agar and API 20 C AUX System. Then the susceptibilities of the strains to amphotericin B and flucoiiazole were determined by microdilution method according to NCCLS M27-A standards. Results: Fortyn seven of the strains were identified ,as C.albicans, fourteen as C.tropicalis, ten as C.parapsilosis, nine as C.glabrata, four as Tricbosporon spp., one as C.guilliermondii and one as C.kejyr. All the strains were found to be susceptible to amphoterisiii B. Fluconazole resistance was detected among C.glabrata strains where as dose dependent susceptible strains were observed among C.albicans, C.tropicalis and C.parapsilosis isolates. Conclusion: Among our intensive care unit isolates., amphoterisin B resistance wasn't detected. However dose dependent susceptibility was found in some strains and fluconazole resistant C.glabrata strains were observed. For this reason, special attention should be given when using fluconazole for the antifungal treatment.

Özet

Amaç: Bu çalışmada yoğun bakım hastalarından soyutlanan maya türlerinin tanımlanması ve antifungal duyarlılıklarının belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmamıza Aralık 2001-Haziran 2002 tarihleri arasında yoğun bakım hastalarının klinik örneklerinden soyutlanan toplam 86 sus alınmıştır. Suşlar çimlenme borusu testi, mısırunu tween 80 agardaki görünümleri ile API 20 C AUX sistemleri kullanılarak tanımlanmıştır. Daha sonra suşların amfoterisin B ve flukonazole duyarlılıkları NCCLS M27-A standartlarına uygun olarak mikrodilüsyon yöntemi ile çalışılmıştır. Bulgular: Soyutlanan suşların 47'si C.albicans, 14'ü C.tropicalis, 10'u C.parapsilosis, 9'u C.glabrata, 4'ü Tricbosporon spp, 1'i C.guilliermondii, 1'i de C.kefyr olarak tanımlanmıştır. Çalışılan tüm suşlar amfoterisin B'ye duyarlı bulunmuştur. Bunun yanında flukonazol için C.glabmta suşlarında direnç belirlenirken, C.albicans, C.tropicalis ve C.parapsilosis türlerinde ise doza bağımlı duyarlılık gözlenmiştir. Sonuç: Yoğun bakım izolatlarımızda amfoterisin B direncinin bulunmadığı ancak bazı suşlarda flukonazole doza bağımlı duyarlılığın, C.glabrata suşlarında ise flukonazole direncin söz konusu olması nedeni ile özellikle flukonazol kullanımında dikkatli davranılması gerektiği düşünülmektedir.

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Cite This Article

YÜCESOY, M., MAROL, S., BİLİRGEN, B., ACARER, V. (2003). Yeast strains isolated from intensive care unit patients and their antifungal susceptibilities. Dokuz Eylül Üniversitesi Tıp Fakültesi Dergisi, Cilt: 17 - Sayı: 1, 1–6. https://ajindex.com/articles/210633

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