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Journals / Genel Tıp Dergisi / 2012 / Cilt: 22 - Sayı: 4

Frequency and genotype distribution of human papillomavirus in cervical specimens

Servikal örneklerde human papillomavirus sıklığı ve genotip dağılımı

DUYGU FINDIK
HATİCE TÜRK DAĞI
UĞUR ARSLAN
Yücel FINDIK
Pages
116–120
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Comprehensive studies participating patient/control groups with similar demographic characteristics and using a standard method are needed for determinination of the prevalence of HPV DNA and HPV types and population covered by HPV vaccine in our country

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Cervical cancer is the second most common type of cancer and the second most common cause of cancer-related deaths in women.
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Key Findings & Data:Results: Out of two hundred and fifty cervical samples included in the study, HPV DNA was detected in 48 (19.2%) samples including 29 (11.6%) only at high-risk, 12 (4.8%) only at low-risk and 7 (2.8%) at high and low risk together.
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Conclusion & Impact:Comprehensive studies participating patient/control groups with similar demographic characteristics and using a standard method are needed for determinination of the prevalence of HPV DNA and HPV types and population covered by HPV vaccine in our country
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Cervical cancer is the second most common type of cancer and the second most common cause of cancer-related deaths in women. Epidemiological studies have shown that human papillomavirus (HPV) is the major risk factor for cervical cancer. The aim of this study was to determine the presence of HPV DNA and HPV genotypes in cervical samples with the request of HPV DNA test. Material and Method: HPV DNA was investigated by PCR method in servical samples. HPV genotyping was performed by reverse hybridization method. Results: Out of two hundred and fifty cervical samples included in the study, HPV DNA was detected in 48 (19.2%) samples including 29 (11.6%) only at high-risk, 12 (4.8%) only at low-risk and 7 (2.8%) at high and low risk together. High-risk HPV genotypes HPV 16 and HPV 30’s+50’s were determined as 18.7%and 12.5%respectively. Conclusion: In our study, HPV 16 which is a high-risk type was detected at the highest rate while other types were detected at different rates. Comprehensive studies participating patient/control groups with similar demographic characteristics and using a standard method are needed for determinination of the prevalence of HPV DNA and HPV types and population covered by HPV vaccine in our country

Özet

Amaç: Servikal kanser kadınlarda ikinci en yaygın kanser tipidir ve kansere bağlı ölümlerin de ikinci en sık nedenidir. Yapılan epidemiyolojik çalışmalar serviks kanseri için majör risk faktörünün human papillomavirus (HPV) olduğunu göstermiştir. Bu çalışmanın amacı HPV DNA araştırılması için laboratuvarımıza gönderilen servikal örneklerde HPV varlığının ve genotiplerinin belirlenmesidir. Gereç ve Yöntem: İncelenen servikal örneklerde PCR yöntemi ile HPV DNA araştırıldı ve revers hibridizasyon yöntemi ile HPV tiplendirilmesi yapıldı. Bulgular: Çalışmaya alınan ikiyüzelli adet servikal örnekten 29'u (%11.6) sadece yüksek riskli, 12'si (%4.8) sadece düşük riskli ve 7'si (%2.8) yüksek ve düşük riskli birlikte pozitif olmak üzere toplam 48 (%19.2) örnekte HPV DNA saptandı. Yüksek riskli HPV genotiplerinden HPV 16 %18.7 ve HPV 30's+50's %1 2.5 oranında tespit edildi. Sonuç: Çalışmamızda yüksek riskli tiplerden olan HPV 16 en yüksek oranda saptanırken diğer tipler ise farklı sıklıklarda belirlenmiştir. Ülkemizde HPV DNA ve HPV tip prevalansının saptanması ve HPV aşısının kapsadığı popülasyonun belirlenmesi için benzer demografik özelliklere sahip hasta/kontrol gruplarının katıldığı ve standart bir yöntemin kullanıldığı geniş kapsamlı çalışmalara ihtiyaç vardır

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Cite This Article

FINDIK, D., DAĞI, H. T., ARSLAN, U., FINDIK, Y. (2012). Frequency and genotype distribution of human papillomavirus in cervical specimens. Genel Tıp Dergisi, Cilt: 22 - Sayı: 4, 116–120. https://ajindex.com/articles/207784

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