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Journals / GORM:Gynecology Obstetrics & Reproductive Medicine / 2013 / Cilt: 19 - Sayı: 2

WNT/Beta - Katenin Sinyal Aktivitesinin İmmünositokimyasal Teknik Kullanılarak Servikal Simirlerde Saptanması

Detection of the WNT/Beta-Catenin Signaling Activity by Using Immunocytochemical Technique in Cervical Smears

HANİFE GÜLER DÖNMEZ
Sayeste DEMİREZEN
Mehmet Sinan BEKSAÇ
Pages
91–95
DOI
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In addition, when signaling was active, the membranous expression of ?-catenin was observed to increase by a statistically significant amount epithelial cells (p

📌 3 Key Takeaways

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Objective & Hypothesis:OBJECTIVES: The Wnt/?-catenin signaling pathway controls cell fate and homeostasis of embryonic and adult tissues.
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Key Findings & Data:STUDY DESIGN: Cervical smears taken from 200 patients were examined in terms of immunostaining of ?-catenin.
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Conclusion & Impact:In addition, when signaling was active, the membranous expression of ?-catenin was observed to increase by a statistically significant amount epithelial cells (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

OBJECTIVES: The Wnt/?-catenin signaling pathway controls cell fate and homeostasis of embryonic and adult tissues. The aim of the study is to evaluate the activity of Wnt/?-catenin signaling in cervical smears by using immunocytochemistry. STUDY DESIGN: Cervical smears taken from 200 patients were examined in terms of immunostaining of ?-catenin. The membranous staining with moderate (++) or strong (+++) cytoplasmic and/or nuclear staining was scored positive for signaling activity. RESULTS: Positive Wnt/?-catenin signaling was found in 21 of 200 cases (10.5%). The nucleus was not found to be positive in any of the cells. However, some nuclear membranes were positive. In addition, when signaling was active, the membranous expression of ?-catenin was observed to increase by a statistically significant amount epithelial cells (p

Özet

AMAÇ: Wnt/?-katenin sinyal yolu hem hücre kaderini hem de embriyonik ve erişkin doku homeostazisini kontrol eder. Ça lış - ma mızın amacı servikal simirlerde Wnt/?-katenin sinyal yolu aktivitesinin immünositokimya kullanılarak değerlendirilmesidir. GEREÇ VE YÖNTEM: 200 hastadan alınan servikal simirler ?- katenin açısından incelendi. Membranöz boyanmayla birlikte orta (++) veya kuvvetli (+++) sitoplazmik ve/veya çekirdek boyanması sinyal yolu aktivitesi açısından pozitif olarak kabul edildi. BULGULAR: 200 olgunun 21'inde (%10.5) Wnt/?-katenin sinyali pozitif olarak bulundu. Hiçbir hücrede çekirdek pozitifliğine rastlanmadı ancak bazı çekirdek zarları pozitifti. Ayrıca, sinyal yolu aktif durumdayken epitel hücrelerde ?-katenin'in zardaki ifadesinin istatistiksel olarak anlamlı bir miktarda arttığı gözlendi. (p<0,05). SONUÇLAR: Çalışmamız rutin servikal simirlerde Wnt/?-katenin sinyal yolu aktivitesinin immünositokimyasal teknikler kullanılarak saptanabileceğini gösterdi.

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Cite This Article

DÖNMEZ, H. G., DEMİREZEN, S., BEKSAÇ, M. S. (2013). WNT/Beta - Katenin Sinyal Aktivitesinin İmmünositokimyasal Teknik Kullanılarak Servikal Simirlerde Saptanması. GORM:Gynecology Obstetrics & Reproductive Medicine, Cilt: 19 - Sayı: 2, 91–95. https://ajindex.com/articles/204328

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References (14)

  1. Kaldis P, Pagano M. Wnt signaling in mitosis, Dev Cell 2009;17:749-50. doi: 10.1016/j.devcel.2009.12. 2. van Amerongen R, Nusse R. Towards an integrated view of Wnt signaling in development Development 2009; 136:3205-14
  2. Logan CY, Nusse R. The Wnt signaling pathway in development and disease, Annu Rev Cell Dev Biol 2004; 20:781-810
  3. Tanir HG, Demirezen Ş, Beksaç MS. Relation with the Wnt/?-catenin signalling pathway with gynaecological cancers. Turk J Biol 2010;34:227-34
  4. Scholten AN, Creutzberg CL, van den Broek LJ, Noordijk EM, Smit VT. Nuclear beta-catenin is a molecular feature of type I endometrial carcinoma. J Pathol 2003;201:460-5
  5. Uren A, Fallen S, Yuan H, Usubütün A, Küçükali T, Schlegel R, et al. Activation of the canonical wnt pathway during genital keratinocyte transformation: a model for cervical cancer progression. Cancer Res 2005;65:6199- 206
  6. Norimatsu Y, Miyamoto M, Kobayashi TK, Moriya T, Shimizu K, Yanoh K, et al. Diagnostic utility of phosphatase and tensin homolog, beta-catenin, and p53 for endometrial carcinoma by thin-layer endometrial preparations. Cancer 2008;114:155-64
  7. Norimatsu Y, Miyamato T, Kobayashi TK, Oda T, Moriya T, Yanoh K, et al. Utility of thin-layer preparations in endometrial cytology: immunocytochemical expression of PTEN, beta-catenin and p53 for benign endometrial lesions. Diagn Cytopathology 2008;36:216-23
  8. Politi EN, Lazaris AC, Kehriotis M, Papathomas TG, Nikolakopoulou E, Koutselini H. Altered expression of adhesion molecules in inflammatory cervical smears. Cytopathology 2008;19:172-8
  9. Huber AH, Weis WI. The structure of the beta-catenin/Ecadherin complex and the molecular basis of diverse ligand recognition by beta-catenin. Cell 2001;105:391-402
  10. Cowin P, Mattey D, Garrod D. Distribution of desmosomal components in the tissues of vertebrates, studied by fluorescent antibody staining. J Cell Sci 1984;66:119-32
  11. Blaskewicz CD, Pudney J, Anderson DJ. Structure and function of intercellular junctions in human cervical and vaginal mucosal epithelia. Biol Reprod 2011;85:97-104
  12. Shinohara A, Yokoyama Y, Wan X, Takahashi Y, Mori Y, Takami T, et al. Cytoplasmic/nuclear expression without mutation of exon 3 of the ?-catenin gene is frequent in the development of the neoplasm of the uterine cervix. Gynecol Oncol 2001;82:450-5
  13. Willert K, Nusse R. Beta-catenin: a key mediator of Wnt signalling. Curr Opin Genet Dev 1998;8:95-102
  14. Giarré M, Semënov MV, Brown AM. Wnt signalin