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Journals / Haydarpaşa Numune Medical Journal / 2020 / Cilt: 60 - Sayı: 3

Prophylactic Effects of Verapamil in Testicular Ischemia-reperfusion Damage in Rats

Zülfü SERTKAYA
Metin İshak ÖZTÜRK
Orhan KOCA
Mehmet Hakan AKYÜZ
Yılmaz Gülistan GÜMRÜKÇÜ
Muhammet İhsan KARAMAN
Pages
279–283
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Verapamil HCl reduces damage in testicular I/R

📌 3 Key Takeaways

🎯
Objective & Hypothesis:are important factors in the occurrence of ischemia-reperfusion damage in testes.
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Key Findings & Data:P<0.05 was statistically significant.
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Conclusion & Impact:Verapamil HCl reduces damage in testicular I/R
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Özet

are important factors in the occurrence of ischemia-reperfusion damage in testes. Verapamil HCl is a phenylalkylamine derivative of the L-type voltage-dependent calcium channel blocker. In our study, we evaluated the prophylactic effects of the Verapamil in testicular ischemia-reperfusion damage. Methods: Twenty-one, eight weeks old adult, male Wistar-Albino rats were randomly divided into three groups as follows: Sham, I/R, I/R+Verapamil HCl. In Sham and I/R groups, saline was injected intraperitoneally at the second hour; in I/R+Verapamil HCl group, Verapamil was injected intraperitoneal at the second hour. Spermatogenic functions were evaluated according to Johnsen criteria, and then, average scores were calculated. P<0.05 was statistically significant. Results: We compared all histopathological and molecular parameters derived from all groups. Average levels of inflammation mediators (TNF-α and IL-1β) were calculated. In the early period, compared with the Sham group, TNF-α levels increased statistically significant in theI/R group, which did not receive any medical treatment. Also, we saw that Verapamil HCl treatment avoided an increase in TNF-α level and provided the same levels as the Sham group. Glutathione peroxidase (GPx), which is antioxidant in tissue levels, significantly decreased in theI/R group, which did not receive Verapamil HCl as compared with the Sham group. Verapamil HCl avoided this situation. Although GPx expression scores were higher in the I/R group than the Sham group, Verapamil HCl treatment takes these scores to the same levels as in the Sham group. For spermatogenesis, there was no statistically significant difference between groups according to Johnsen scoring system. Discussion and Conclusion: I/R generated by T/D, in the early period, causes an increase of inflammation mediators in blood. It resulted in an increase in GPx activity, which decreased anti- oxidant capacity in tissue, and it has no effect to spermatogenesis parameters in four hours period. Verapamil HCl reduces damage in testicular I/R

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Cite This Article

SERTKAYA, Z., ÖZTÜRK, M. İ., KOCA, O., AKYÜZ, M. H., GÜMRÜKÇÜ, Y. G., KARAMAN, M. İ. (2020). Prophylactic Effects of Verapamil in Testicular Ischemia-reperfusion Damage in Rats. Haydarpaşa Numune Medical Journal, Cilt: 60 - Sayı: 3, 279–283. https://ajindex.com/articles/199682

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References (17)

  1. Barada JH, Weingarten JL, Cromie WJ. Testicular salvage and age-related delay in the presentation of testicular torsion. J Urol 1989;142:746–8
  2. Cattolica EV, Karol JB, Rankin KN, Klein RS. High testicular salvage rate in torsion of the spermatic cord. J Urol 1982;128:66–8
  3. Mogilner JG, Lurie M, Coran AG, Nativ O, Shiloni E, Sukhotnik I. Effect of diclofenac on germ cell apoptosis following testicular ischemia-reperfusion injury in a rat. Pediatr Surg Int 2006;22:99–105
  4. Pentyala S, Lee J, Yalamanchili P, Vitkun S, Khan SA. Testicular torsion: a review. J Low Genit Tract Dis 2001;5:38–47
  5. Filho DW, Torres MA, Bordin AL, Crezcynski-Pasa TB, Boveris A. Spermatic cord torsion, reactive oxygen and nitrogen species and ischemia-reperfusion injury. Mol Aspects Med 2004;25:199–210
  6. Visser AJ, Heyns CF. Testicular function after torsion of the spermatic cord. BJU Int 2003;92:200–3
  7. Jun P, Ko NU, English JD, Dowd CF, Halbach VV, Higashida RT, et al. Endovascular treatment of medically refractory cerebral vasospasm following aneurysmal subarachnoid hemorrhage. AJNR Am J Neuroradiol 2010;31:1911–6
  8. Anderson JB, Williamson RC. The fate of the human testes following unilateral torsion of the spermatic cord. Br J Urol 1986;58:698–704
  9. Turner TT, Bang HJ, Lysiak JL. The molecular pathology of experimental testicular torsion suggests adjunct therapy to surgical repair. J Urol 2004;172:2574–8
  10. Riedemann NC, Ward PA. Complement in ischemia reperfusion injury. Am J Pathol 2003;162:363–7
  11. Görlach A, Bertram K, Hudecova S, Krizanova O. Calcium and ROS: A mutual interplay. Redox Biol 2015;6:260–71
  12. Cooper BJ. Disease at the cellular level. In: Kitt T (editor). Text Book of Comparative General Pathology. ST. Louis: Mosby Company; 2002. p. 16–75
  13. Pekcetin C, Ergur BU, Kiray M, Bagriyanik A, Tugyan K, Erbil G, et al. The protective effects of trimetazidine on testicular ischemia and reperfusion injury in rats. Pediatr Surg Int 2007;23:1113–8
  14. Ergur BU, Kiray M, Pekcetin C, Bagriyanik HA, Erbil G. Protective effect of erythropoietin pretreatment in testicular ischemiareperfusion injury in rats. J Pediatr Surg 2008;43:722–8
  15. Wei SM, Yan ZZ, Zhou J. Beneficial effect of taurine on testicular ischemia-reperfusion injury in rats. Urology 2007;70:1237–42
  16. Wei SM, Yan ZZ, Zhou J. Curcumin attenuates ischemia-reperfusion injury in rat testis. Fertil Steril 2009;91:271–7
  17. Beheshtian A, Salmasi AH, Payabvash S, Kiumehr S, Ghazinezami