AI Summary & Key Takeaways
AI InsightWidespread use of these parameters may be beneficial for the early diagnosis and effective treatment of RA and similar diseases resulting in bone deformities in the future.
📌 3 Key Takeaways
Abstract
Objectives: This study aims to evaluate the biomarkers of bone formation and resorption to determine the mechanism of bone deformities in patients with rheumatoid arthritis (RA). Patients and methods: Thirty patients (9 males, 21 females; mean age, 51.5 years; range 23 to 73 years) with RA and 19 healthy individuals (7 males, 12 females; mean age 51 years; range 24 to 61 years) were included in this study. Patients with RA were subgrouped according to disease activity (active, n=21, mean age 53.0; range 37 to 73 years; inactive, n=9, mean age 46.0; range 23 to 62 years). Levels of receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), tartrate-resistant acid phosphatase-5b, tumor necrosis factor-alpha (TNF-α), cathepsin-K, and activity of matrix metalloproteinase-3 (MMP-3) were measured in patient and control groups with enzyme-linked immunosorbent assay method. Results: Levels of RANKL, OPG, cathepsin-K, TNF-α and MMP-3 activities of the patients were significantly higher when compared with the controls (p<0.05). A comparison of the active and inactive patients with RA revealed that OPG levels and MMP-3 activities were significantly higher in active group than inactives (p<0.05), while there was no significant difference in TNF-α, RANKL levels, and tartrate-resistant acid phosphatase-5b and cathepsin-K activities between two groups (p>0.05). Conclusion: Increased RANKL, OPG, TNF-α, cathepsin-K levels and MMP-3 activities in RA patients demonstrated that these parameters are related to bone deformities which occur in the process of RA. Widespread use of these parameters may be beneficial for the early diagnosis and effective treatment of RA and similar diseases resulting in bone deformities in the future.
Özet
Amaç:/b> Bu çalışmada romatoid artritli (RA) hastalarda kemik deformite mekanizmalarının belirlenmesi amacıyla kemik yapım ve yıkım belirteçleri değerlendirildi. Hastalar ve yöntemler: RAlı 30 hasta (9 erkek, 21 kadın; ort. yaş 51.5 yıl; dağılım 23-73 yıl) ve 19 sağlıklı birey (7 erkek, 12 kadın; ort. yaş 51 yıl; dağılım 24-61 yıl) çalışmaya dahil edildi. RAlı hastalar hastalık aktivitesine göre alt gruplara ayrıldı (aktif, n=21, ort. yaş 53.0; dağılım 37-73 yıl; inaktif, n=9, ort. yaş 46.0; dağılım 23-62 yıl). Hasta ve kontrol gruplarında reseptör aktivatör nükleer faktör kappa ligand (RANKL), osteoprotegerin (OPG), tartrat rezistan asit fosfataz-5b, tümör nekroz faktör alfa (TNF-α), katepsin K seviyeleri ve matriks metalloproteinaz-3 (MMP-3) aktivitesi enzime bağlı immünosorban yöntemi ile ölçüldü. Bulgular: Hastaların RANKL, OPG, katepsin-K, TNF-α seviyeleri ve MMP-3 aktiviteleri kontrollere kıyasla anlamlı olarak yüksekti (p 0.05), iki grup arasında TNF-α, RANKL seviyeleri ile tartrat rezistan asit fosfataz-5b ve katepsin-K aktiviteleri açısından anlamlı fark yoktu (p<0.05). Sonuç: RAlı hastalarda artmış RANKL, OPG, TNF-α, katepsin-K seviyeleri ile MMP-3 aktiviteleri, bu parametrelerin RA sürecinde meydana gelen kemik deformiteleri ile ilişkili olduğunu gösterdi. Bu parametrelerin yaygın kullanımı, gelecekte RAnın ve kemik deformiteleri ile sonuçlanan benzer hastalıkların erken tanı ve etkin tedavisinde yararlı olabilir.