Phytochemical characterisation of Phlomis linearis Boiss. & Bal and screening for anticholinesterase, antiamylase, antimicrobial, and cytotoxic properties

To the best of our knowledge, the present work is the first comprehensive report on anti-acetylcholinesterase, anti-α-amylase, and antimicrobial activities, as well as cytotoxic effects of P. linearis.

📌 3 Key Takeaways

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Objective & Hypothesis:Öz In the present work, essential oil and fatty acids and extracts obtained from aerial parts of Phlomis linearis Boiss. & Bal. were investigated for chemical composition and biological activities.

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Key Findings & Data:The essential oil (EO) contained α-pinene (12.5%) and β-caryophyllene (10.7%) as main compounds.

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Conclusion & Impact:To the best of our knowledge, the present work is the first comprehensive report on anti-acetylcholinesterase, anti-α-amylase, and antimicrobial activities, as well as cytotoxic effects of P. linearis.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Anahtar Kelimeler Phlomis linearis · essential oil · extract

Özet

Öz In the present work, essential oil and fatty acids and extracts obtained from aerial parts of Phlomis linearis Boiss. & Bal. were investigated for chemical composition and biological activities. The phytochemical analyses were conducted with gas chromatographymass spectrometry/flame ionisation detector (GC-MS/FID) and liquid chromatography-mass spectromtetry (LC-MS/MS) techniques. The extracts and essential oil were studied for α-amylase and acetylcholinesterase activities with two different spectrophotometric methods. Antimicrobial activities of the extracts were investigated by microdilution. The extracts were evaluated in vitro for cytotoxic effects against cancer and normal cell lines by MTT assay. The essential oil (EO) contained α-pinene (12.5%) and β-caryophyllene (10.7%) as main compounds. Palmitic (26.5%) and nonadecanoic acids (26.6%) were determined as fatty acids. Phytochemical analysis of the extracts found phenolic acids, phlinosides, verbascoside, and flavonoids. The extracts and essential oil demonstrated poor α-amylase inhibitory activity. The best acetylcholinesterase inhibitory activity was obtained for diethly ether extract of P. linearis (67.2 ± 3.4%) at 10 mg /mL concentration. Ethyl acetate extract found to be effective against Staphlococcus aureus at a minimum inhibitory concentration (MIC) of 156.26 μg/mL. Diethyl ether extract of P. linearis was active on A549 cell lines with an IC50 = 316 ± 4.16 μg/mL when compared with cisplatin IC50 = 24.43 ± 0.14 μg/mL. To the best of our knowledge, the present work is the first comprehensive report on anti-acetylcholinesterase, anti-α-amylase, and antimicrobial activities, as well as cytotoxic effects of P. linearis.

Keywords

Anahtar Kelimeler Phlomis linearis essential oil extract liquid chromatography-mass spectromtetry (LC-MS/MS)gas chromatography-mass spectrometry/flame ionisation detector (GC-MS/FID)activity

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Cite This Article

GOGER, G., TÜRKYOLU, Ü., GÜRŞEN, E. N., YUR, S., KARADUMAN, A. B., GÖGER, F., TEKİN, M., ÖZEK, G. (2021). Phytochemical characterisation of Phlomis linearis Boiss. & Bal and screening for anticholinesterase, antiamylase, antimicrobial, and cytotoxic properties. Turkish Journal of Chemistry, Cilt: 45 - Sayı: 2, 387–399. https://ajindex.com/articles/198089

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References (5)

Abdeislam NM, 2013, LIFE SCI J, V10. Ablajan K, 2006, J MASS SPECTROM, V41, P352, DOI 10.1002/jms.995 Aghakhani F, 2018, PHYTOCHEM ANALYSIS, V29, P180, DOI 10.1002/pca.2733 Ahmed MF, 2010, INT J ENDOCRINOL, V2010, DOI 10.1155/2010/841090 Alpay M, 2019, AN ACAD BRAS CIENC, V91, DOI 10.1590/0001-3765201920180404 Altun ML, 2013, IND CROP PROD, V43, P87, DOI 10.1016/j.indcrop.2012.07.017 Babushok VI, 2011, J PHYS CHEM REF DATA, V40, DOI 10.1063/1.3653552 Basaran AA, 1991, HACETTEPE U ECZACILI, V11, P81 Basta A, 2006, FLAVOUR FRAG J, V21, P795, DOI 10.1002/ffj.1717 Brophy JJ, 2004, BIOCHEM SYST ECOL, V32, P867, DOI 10.1016/j.bse.2004.05.001 Calis I, 2004,
PHYTOCHEMISTRY, V65, P2619, DOI 10.1016/j.phytochem.2004.04.038 CALIS I, 1991,
PHYTOCHEMISTRY, V30, P3073, DOI 10.1016/S0031-9422(00)98255-4 CALIS I, 1990,
PHYTOCHEMISTRY, V29, P1253 Clifford MN, 2005, J AGR FOOD CHEM, V53, P3821, DOI 10.1021/jf050046h Clifford MN, 2003, J AGR FOOD CHEM, V51, P2900, DOI 10.1021/jf026187q Clinical and Laboratory Standards Institute (CLSI), 2008, 27A3 CLSI, V3 Clinical and Laboratory Standards Institute (CLSI), 2006, M7A7 CLSI, V7th Couladis M, 2000, PLANTA MED, V66, P670, DOI 10.1055/s-2000-8631 da Silva JKR, 2014, IND CROP PROD, V58, P55, DOI 10.1016/j.indcrop.2014.04.006 Demirci B, 2003, Z NATURFORSCH C, V58, P826 EDQM, 2005, EUROPEAN PHARMACOPEA, V1, P217 El Mokni R, 2019, BIOMED CHROMATOGR, V33, DOI 10.1002/bmc.4603 ELLMAN GL, 1961, BIOCHEM PHARMACOL, V7, P88, DOI 10.1016/0006-2952(61)90145-9 Ferreres F, 2011, RAPID COMMUN MASS SP, V25, P700, DOI 10.1002/rcm.4910 Formisano C, 2006, FLAVOUR FRAG J, V21, P848, DOI 10.1002/ffj.1740 Gandhiraja N, 2009, ETHNOBOTANICAL LEAFL, V2009, P8 Goger F, 2019, J TURKISH CHEM SOC A, V6, P281 Goger F, 2015, BANGL J PHARMACOL, V10, P639, DOI 10.3329/bjp.v10i3.23500 Goger G, 2018, IND CROP PROD, V120, P279, DOI 10.1016/j.indcrop.2018.04.024 Greenwell M, 2015, INT J PHARM SCI RES, V6, P4103, DOI 10.13040/IJPSR.0975-8232.6(10).4103-12 Guimaraes R, 2013, FOOD CHEM, V141, P3721, DOI 10.1016/j.foodchem.2013.06.071 Iscan G, 2002, J AGR FOOD CHEM, V50, P3943, DOI 10.1021/jf011476k Jabeen B, 2013,
PHYTOCHEMISTRY, V96, P443, DOI 10.1016/j.phytochem.2013.09.015 Joshi RK, 2011, SCI WORLD, V9, P63, DOI DOI 10.3126/SW.V9I9.5521 Justesen U, 2000, J CHROMATOGR A, V902, P369, DOI 10.1016/S0021-9673(00)00861-X Kalaivani R, 2012, J BIOL ACTIVE PRODUC, V2, P30 Karadag AE, 2020, J ESSENT OIL RES, V32, P242, DOI 10.1080/10412905.2020.1743786 Kim JS, 2000, BIOSCI