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Journals / Ege Tıp Dergisi / 2017 / Cilt: 56 - Sayı: 4

Ellagic acid induced expression of miRNAs in breast cancer stem cells and effect ofellagic acid on apoptosis

Meme kanseri kök hücrelerinde elajik asit ile indüklenmiş miRNA'ların ifadesi ve elajik asidin apoptoz üzerine etkisi

Hasan Onur ÇAĞLAR
SUNDE YILMAZ SÜSLÜER
Şebnem KAVAKLI
CUMHUR GÜNDÜZ
Biray ERTÜRK
FERİŞTAH FERDA ÖZKINAY
Ayfer HAYDAROĞLU
Pages
183–192
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Expression profiles of all the others miRNAs, including members of Let-7 and miR-200 families, showed a decrease due to EA treatment.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: miRNAs which are associated with estrogen dependent tumorigenesis of breast cancer are suppressed by ellagic acid (EA) treatment.
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Key Findings & Data:Materials and Methods: Cytotoxic effects of EA on BCSCs were examined by WST-1 reagent test.
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Conclusion & Impact:Expression profiles of all the others miRNAs, including members of Let-7 and miR-200 families, showed a decrease due to EA treatment.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: miRNAs which are associated with estrogen dependent tumorigenesis of breast cancer are suppressed by ellagic acid (EA) treatment. However, modulation of expression profiles of miRNAs in breast cancer stem cells (BCSCs) after EA treatment is still unclear. In this study, it was aimed to show EA-induced apoptosis in BCSCs and to determine altered expression profiles of miRNAs after EA treatment. Materials and Methods: Cytotoxic effects of EA on BCSCs were examined by WST-1 reagent test. Apoptosis and cell cycle analysis after EA treatment were detected by flow cytometry analysis. After EA treatment, miRNA expression profiles of BCSCs were determined by RT-PCR miRNA array. Results: Cytotoxic effect of EA on BCSCs was found. IC50 concentration of EA at 48th and 72nd hours was 24.8 ?M. EA did not induce apoptosis in BCSCs. In addition, S phase arrest was observed at 24th, 48th, 72nd hours of EA treatment. Expression profiles of 76 of totally 84 miRNA genes were detected. Expression of hsa-miR-125b-1-3p was increased as 3.59 folds by EA treatment. Expression profiles of all the others miRNAs, including members of Let-7 and miR-200 families, showed a decrease due to EA treatment.

Özet

Östrojen bağımlı meme kanseri tümör oluşumunda etkili miRNA'lar elajik asit (EA) uygulaması ile baskılanmaktadır. Ancak, EA uygulamasından sonra meme kanseri kök hücrelerindeki (MKKH) miRNA'ların ifade profillerinin düzenlenmesi hala belirsizdir. Bu çalışmada, MKKH'lerde EA-indüklenmiş apoptozun gösterilmesi ve EA uygulamasından sonra değişen miRNA ifade profilinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: EA'nın MKKH'lerdeki sitotoksik etkisi WST-1 testi ile incelendi. EA uygulamasından sonra apoptoz ve hücre döngüsü analizleri flow sitometri ile yapıldı. EA uygulamasından sonra, MKKH'lerin miRNA ifade profilleri RT-PCR miRNA array ile tanımlandı. Bulgular: MKKH'ler üzerinde EA'nın sitotoksik etkisi saptandı. 48. ve 72. saatlerde EA'nın IC50 konsantrasyonu 24.8 ?M'dı. EA, MKKH'lerde apoptozu indüklemedi. Bununla beraber, EA'nın 24., 48. ve 72. saat uygulamalarında S fazı bloğu gözlendi. Toplamda 84 miRNA geninin 76'sının ifade profili belirlendi. hsa-miR-125b-1-3p'nin ifadesi EA uygulanmasıyla 3.59 kat arttı. Let-7 ve miR-200 aileleri dahil diğer tüm miRNA'ların ifade profili EA uygulaması nedeniyle azalış gösterdi. Sonuç: Bu çalışmada, EA'nın MKKH'de apoptozu etkilemediği ve hsa-miR-125b-1-3p'in ifadesini arttırırken, başta hsa-miR-485-5p ve hsa-miR-328-3p olmak üzere diğer miRNA'ların ifadesinde düşmeye neden olduğu görüldü

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Cite This Article

ÇAĞLAR, H. O., SÜSLÜER, S. Y., KAVAKLI, Ş., GÜNDÜZ, C., ERTÜRK, B., ÖZKINAY, F. F., HAYDAROĞLU, A. (2017). Ellagic acid induced expression of miRNAs in breast cancer stem cells and effect ofellagic acid on apoptosis. Ege Tıp Dergisi, Cilt: 56 - Sayı: 4, 183–192. https://ajindex.com/articles/190215

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