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Journals / Dokuz Eylül Üniversitesi Tıp Fakültesi Dergisi / 2010 / Cilt: 24 - Sayı: 1

Evaluation of an enzyme immunassay using recombinant antigens for syphilis screening

Rekombinant antijenler kullanan bir enzim “immunassay” testinin sifiliz taraması için değerlendirilmesi

ÖZGEN ALPAY ÖZBEK
YAVUZ DOĞAN
İlknur Ebru AKYÜZ AKÇAKANAT
Pages
19–24
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: It was concluded that evaluated EIA test is easy to use and sensitive enough for syphilis screening.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: The aim of this study is to evaluate the performance of an enzyme immunoassay (Trep-Screen ELISA) using recombinant antigens for determining anti- Treponema pallidum antibodies in screening syphilis.
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Key Findings & Data:The first group composed of 79 samples which were positive in the chemiluminescent microparticle immunoassay (CMIA) screening and were confirmed with Treponema pallidum haemagglutination assay, Western Blot IgM and IgG.
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Conclusion & Impact:Conclusion: It was concluded that evaluated EIA test is easy to use and sensitive enough for syphilis screening.
🔬Design:Research Article
📍Scope:79 samples
⚙️Variables:Domain Parameters

Abstract

Objective: The aim of this study is to evaluate the performance of an enzyme immunoassay (Trep-Screen ELISA) using recombinant antigens for determining anti- Treponema pallidum antibodies in screening syphilis. Material and method: Two group of samples were used in the study. The first group composed of 79 samples which were positive in the chemiluminescent microparticle immunoassay (CMIA) screening and were confirmed with Treponema pallidum haemagglutination assay, Western Blot IgM and IgG. The second group included 71 samples which were assessed to be positive in the CMIA screening but were not confirmed by the other tests. All of the samples were tested by evaluated EIA. Results: the sensitivity of the test was calculated as 97.5% (95% contidence internal, 90.3%-99.6%). With the EIA, 73.2% of the results were negative and 26.8% of the re-sults were positive when second group samples were tested. Conclusion: It was concluded that evaluated EIA test is easy to use and sensitive enough for syphilis screening.

Özet

Amaç: Bu çalışmada, anti-Treponema pallidum antikorlarını saptamak için rekom- binant antijen kullanan ve enzim “immunoassay” (EIA) prensibi ile çalışan bir testin (Trep-Screen ELISA) sifiliz taramasındaki performansının değerlendirilmesi amaçlanmıştır. Gereç ve yöntem: Çalışmada, 2 örnek grubu kullanıldı. Birinci grupta kemilüminesan mikropartikül enzim “immunoassay” (CMIA) yöntemi ile yapılan taramada pozitif bulunan ve Treponema pallidum hemaglütinasyon, Western Blot IgM ve IgG testleri ile doğrulanan 79 örnek yer aldı. İkinci grupta CMIA taramasında pozitif bulunan ancak diğer testlerle doğrulanmayan 71 örnek değerlendirildi. Örneklerin tümü değerlendirilen EIA testi ile çalışıldı. Bulgular: Testin duyarlılığı %97,5 (%95 güvenlik aralığı, %90,3-%99,6) olarak bulundu. EIA testinin ikinci gruptaki örneklerin %73,2’sinde negatif, %26,8’inde pozitif sonuç verdiği belirlendi. Sonuç: Değerlendirmeye alınan EIA testinin kullanımı kolay ve sifiliz taraması için yeterli duyarlılığa sahip olduğu sonucuna varıldı.

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Cite This Article

ÖZBEK, Ö. A., DOĞAN, Y., AKÇAKANAT, İ. E. A. (2010). Evaluation of an enzyme immunassay using recombinant antigens for syphilis screening. Dokuz Eylül Üniversitesi Tıp Fakültesi Dergisi, Cilt: 24 - Sayı: 1, 19–24. https://ajindex.com/articles/190189

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