The Determination of Antimicrobial Susceptibilities of Lactococcus garvieae Strains

Objective & Hypothesis:This study was to investigated antimicrobial susceptibilities by disc diffusion method on MuellerHinton agar and ATB VET (14 289 Biomerieux) strip system and also the value the minimum inhibitory concentration (MIC) of Eritromycine antibiotic by Etest (ABBIODISK) in 9 strains of Lactococcus garvieae.

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Key Findings & Data:L.garvieae strains were determined as sensitive to Amoxicillin+clavulanic acid, Ampicillin, Enrofloxacin, Vancomycin, Tetracycline, Doxycycline, Chloramphenicol, Erytromycine, Nitrofurantoin, Cephalotin, Amoxicillin, Cefoperazon, Spectinomycin, Pristinamycin, Cotrimoxazol, and resistant to Kanamycin, Cefuroxime, Lincomyc n, Penicillin, Ofloxacin, Norfloxacin,Ciprofloxacin Ceftriaxone, Clindamycine, Vancomycin, Oxacillin Streptomycin, Trimethoprim+ Sulfamethoxazole, Gentamicin, Apramycin, Tylosin, Colistin, Sulfamethizol, Flumequin, Oxolinic acid by disc diffusion method and ATB VET (14 289 Biomerieux) strip system.

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Conclusion & Impact:The values showed to sensitive of eritromycine antibiotic for all strains

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Lactococcus garveiae · antimikrobiyal duyarlılık · minimum inhibitör konsantrasyonu (MIK)

Abstract

This study was to investigated antimicrobial susceptibilities by disc diffusion method on MuellerHinton agar and ATB VET (14 289 Biomerieux) strip system and also the value the minimum inhibitory concentration (MIC) of Eritromycine antibiotic by Etest (ABBIODISK) in 9 strains of Lactococcus garvieae. L.garvieae strains were determined as sensitive to Amoxicillin+clavulanic acid, Ampicillin, Enrofloxacin, Vancomycin, Tetracycline, Doxycycline, Chloramphenicol, Erytromycine, Nitrofurantoin, Cephalotin, Amoxicillin, Cefoperazon, Spectinomycin, Pristinamycin, Cotrimoxazol, and resistant to Kanamycin, Cefuroxime, Lincomyc n, Penicillin, Ofloxacin, Norfloxacin,Ciprofloxacin Ceftriaxone, Clindamycine, Vancomycin, Oxacillin Streptomycin, Trimethoprim+ Sulfamethoxazole, Gentamicin, Apramycin, Tylosin, Colistin, Sulfamethizol, Flumequin, Oxolinic acid by disc diffusion method and ATB VET (14 289 Biomerieux) strip system. MIC values of Eritromycin antibiotic were obtained as 0.032-0.125 µg/mL in L. Garvieae strains. The values showed to sensitive of eritromycine antibiotic for all strains

Özet

Bu çalışmada, 9 farklı Lactococcus garveiae suşlanmn Mueller-Hinton agarda disk diffüzyon tekniği ile ATB VET (Biomerieux 14 289) strip sistemi kullanılarak antimikrobiyal duyarlılıklarının yanısıra E testi (AB BIODISK) ile de Eritromycin antibiyotiğinin MIK(Minimal inhibitör konsantrasyonu) değerleri incelenmiştir. L.garvieae suşlarının; disk diffüzyon testi ve ATB VET sistemine göre Amoxicillin+clavulanic acid, Ampicillin, Enrofloxacin, Vancomycin, Tetracycline, Doxycycline, Chloramphenicol, Erytromycine, Nitrofurantoin, Cephalotin, Amoxicillin, Cefoperazon, Spectinomycin, Pristinamycin, Cotrimoxazol, antibiyotiklerine duyarlı oldukları, Kanamycin, Cefuroxime, Lincomycin, Penicillin, Ofloxacin, Norfloxacin,Ciprofloxacin Ceftriaxone, Clindamycine, Vancomycin, Oxacillin, Streptomycin, Trimethoprim+ Sulfamethoxazole, Gentamicin, Apramycin, Tylosin, Colistin, Sulfamethizol, Flumequin, Oxolinic asit antibiyotiklerine dirençli oldukları tespit edilmiştir. L. garvieae suşlarında eritromisin antibiyotiğinin MIK değerleri 0,032-0,125 gg/mL olarak tespit edilmiştir. Elde edilen bu değerlere göre; suşların eritromisine duyarlı olduğu görülmüştür.

Keywords

Lactococcus garveiae antimikrobiyal duyarlılık minimum inhibitör konsantrasyonu (MIK)antimicrobial susceptibilities minimum inhibitory concentration (MIC)

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Cite This Article

KUBILAY, A., ALTUN, S., ULUKÖY, G., DILER, Ö. (2005). The Determination of Antimicrobial Susceptibilities of Lactococcus garvieae Strains. Acta Aquatica Turcica, Cilt: 1 - Sayı: 1. https://ajindex.com/articles/18627

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References (10)

Altun S, Diler O, Adilo lu AK (2004). Genotyping of Lactococcus garvieae strains from rainbow trout (Oncorhynchus mykiss) by 16S rDNA sequencing. Bull. Eur. Ass. Fish Pathol 24,119-125.
Arda, M.,(1997) Temel Mikrobiyoloji. Medisan yayn seri No:25.Ankara.490s. Austin B, Van Pouce A (1993). A Gram-positive lacticacid bacterium causing steady mortalities in farmed rainbow trout, Oncorhynchus mykiss Walbaum, in the UK. Bull.Eur.Assoc.Fish Pathol. 13,114-118.
Austin, B., Austin, D.A. (1999) Bacterial fish pathogens disease of farmed and wild fish third (Revised) Edition.
Praxis Publishing Chichester, U.K., 457p. Bragg, R., Broero, J.S.E. (1986) Streptococcosis in rainbow trout in South Africa. Bull. Ass. Fsih Parhol. 6, 89-91 Ça rgan, H and Tanrkul, T. T. (1997) A Lactococcus in a trout farm. Mediterranean Fisheries Congress. 9-11
April Izmir, Turkey, 40p. Carson, J., Gudkovs, N., Austin, B.,(1993) Characteristic of an Enterococcus-like bacterium from Australia and South Africa, pathogenic for rainbow trout, (Oncorhynchus mykiss) (Walbaum). Journal of Fish Dieases. 16, 381-388
Ceschia, G., Girgetti, G., Giavenni, R., Sarti, M.(1992) A new problem for Italian trout farms: Streptococcosis in rainbow (Oncorhynchus mykiss). Bull. Eur. Ass. Fish. Pathol., 12:71-72.
Chang, P. H., Lin, C. W. and Lee, C. (2002) Lactococcus garvieae infection of cultured rainbow trout, O. mykiss in Taiwan and associated biophysical characteristics and histopathology. Bull. Eur. Ass. Fish Pathol., 25 (5), 319- 327. Chen, S.C., Lin, Y.D., Liaw, L.L. and Wang, P.C.(2001) Lactococcus garvieae infection in the giant freshwater prawn Macrobrachium rosenbergii confirmed by polymerase cahin reaction and 16SrDNA sequencing. Dis. Aquat. Org. 45:45-52.
Collins, C. H., Lyne, P.M. (1976) Microbiological methods. Butterworths. 521p. Cowan, S. T., Steel, K. J. (1970). Manual for the identification of medical bacteria. Cambridge of University Press, 216 p. Diler, Ö., Altun, S., Adilo lu, A.K., Kubilay, A. and Ikl, B.I. (2002) First occurence of Streptococcosis affecting farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Bull. Eur. Ass. Fish Pathol., 22:21-26.
Eldar, A.C., Ghittino, C., Asanta, L., Bvozzettz, E., Goria, M. (1996) Enterococcus seriolicida is a Junior synonym of Lactococcus garvieae, a causative agent of septicemia and meningoencephalitis in Fish. Curr. Microbiol. 32:85-88.
Eldar, A. and Ghittino, C. (1999) Lactococcus garvieae and Streptococcus iniae infections in rainbow trou