Journals / Acta Aquatica Turcica / 2005 / Cilt: 1 - Sayı: 1

Cryopreservat on of Rainbow Trout (Oncorhynchus mykiss) and Mirror Carp (Cyprinus carpio) Sperm with Glucose Based Extender

Gökkuşağı Alabalığı (Oncorhynchus mykiss) ve Aynalı Sazan (Cyprinus carpio) Spermasının Glukoz Tabanlı Sulandırıcı ile Dondurulması

Pages
—
Publication Date
2005-01-04
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In rainbow trout, the best post-thaw motility (%), post-thaw movement duration (s) and post-thaw fertilization results (%) were obtained as 57 %, 96 s. and 68.5 % while the same properties were determined as 64 %, 218 s. and 35 % in mirror carp respectively

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, cryopreservation of rainbow trout (Oncorhynchus mykiss) and mirror carp (Cyprinus carpio) spermatozoa was investigated.
📊
Key Findings & Data:Diluted sperm was packaged in 0.5 ml straws and frozen in liquid nitrogen vapour (-196 °C) for 10 minutes and plunged into the liquid nitrogen.
💡
Conclusion & Impact:In rainbow trout, the best post-thaw motility (%), post-thaw movement duration (s) and post-thaw fertilization results (%) were obtained as 57 %, 96 s. and 68.5 % while the same properties were determined as 64 %, 218 s. and 35 % in mirror carp respectively
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Gökkuşağı alabalığı (Oncorhynchus mykiss) · aynalı sazan (Cyprinus carpio) · sperma

Abstract

In this study, cryopreservation of rainbow trout (Oncorhynchus mykiss) and mirror carp (Cyprinus carpio) spermatozoa was investigated. Following determination of the spermatological properties, the pooled ejaculates were diluted with glucose based extender. Diluted sperm was packaged in 0.5 ml straws and frozen in liquid nitrogen vapour (-196 °C) for 10 minutes and plunged into the liquid nitrogen. The straws were thawed in a water bath at 30 ºC for 30 seconds. In rainbow trout, the best post-thaw motility (%), post-thaw movement duration (s) and post-thaw fertilization results (%) were obtained as 57 %, 96 s. and 68.5 % while the same properties were determined as 64 %, 218 s. and 35 % in mirror carp respectively

Özet

Bu çalışmada, gökkuşağı alabalığı (Oncorhynchus mykiss) ve aynalı sazan (Cyprinus carpio) spermasının dondurulması araştırıldı. Spermatolojik özelliklerin belirlenmesinin ardından, ejakulatlar glukoz tabanlı sulandırıcı ile sulandırıldı. Sulandırılan sperma 0,5 ml’lik payetlere çekilerek sıvı azot buharında (-196 °C) 10 dakika süre ile donduruldu ve sıvı azot içerisine aktarıldı. Payetler 30 °C su banyosunda 30 saniye süre ile çözüldü. Gökkuşağı alabalığında çözülme sonu en iyi motilite (%), canlılık süresi (s) ve döl verimi yüzdesi sırasıyla % 57, 96 s. ve % 68,5 olarak elde edilirken aynı özellikler aynalı sazanda sırasıyla % 64, 218 s. ve % 35 olarak belirlendi.

🔖

Cite This Article

BOZKURT, Y., SEÇER, S., TEKİN, N., AKÇAY, E. (2005). Cryopreservat on of Rainbow Trout (Oncorhynchus mykiss) and Mirror Carp (Cyprinus carpio) Sperm with Glucose Based Extender. Acta Aquatica Turcica, Cilt: 1 - Sayı: 1. https://ajindex.com/articles/18624

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Akçay, E., Bozkurt, Y., Kayam, S. 2002. Cryopreservation of mirror carp (Cyprinus carpio L. 1758) sperm: with emphasis on post-thaw motility. In: 1st Int. Cong. Aquacult.-Fish. Technol. Environ. Management., Athens, Greece p. 5. Akçay, E., Bozkurt Y., Seçer S., Tekin, N. 2004a. Cryopreservation of mirror carp semen Turk. J. Vet. Anim. Sci. 28 (4) 837-843.
  2. Akçay, E., Bozkurt, Y., Seçer, S. Tekin, N. 2004b. Pelet yöntemiyle dondurulan gökku"aA! alabal!A! spermas!nda gliserol ilavelerinin fertilizasyon üzerine etkisi. 3. Ulusal Reprodüksiyon ve Sun’i Tohumlama Kongresi, 30 Eylül - 02 Ekim Antalya, 104. Benau, D., Terner, C. 1980. Initiation, prolongation and reactivation of the motility of salmonid spermatozoa. Gamete Res., 3: 247-257.
  3. Billard, R. 1992. Reproduction in rainbow trout. Aquaculture, 100: 263-298.
  4. Bozkurt, Y., Seçer, S. 2004. Aynal! sazan (Cyprinus carpio L. 1758) spermas!n!n baz! spermatolojik özelliklerinin belirlenmesi ve farkl! suland!r!c!lar ile k!sa süreli saklanmas!. 3. Ulusal Reprodüksiyon ve Sun’i Tohumlama Kongresi, 30 Eylül - 02 Ekim Antalya, 106. Bozkurt, Y., Akçay, E., Tekin, N., Seçer, S. 2005a. Effect of freezing techniques, extenders and cryoprotectants on the fertilization rate of frozen rainbow trout (Oncorhynchus mykiss) sperm. The Israeli Journal of AquacultureBamidgeh, 57 (2) 125-130.
  5. Bozkurt, Y., Seçer, S. 2005b. Effect of short-term preservation of mirror carp (Cyprinus carpio) semen on motility, fertilization and hatching rates. The Israeli Journal of Aquaculture-Bamidgeh, 57 (3) 207-212.
  6. Ciereszko, A., Dabrowski, K. 1993. Estimation of sperm concentration of rainbow trout, whitefish and yellow perch using a spectrophotometric technique. Aquaculture, 109: 367-373.
  7. Geffen, A.J., Evans, J. 2000. Sperm traits and fertilization success of male and sex reversed female rainbow trout., Aquaculture, 182: 61-72.
  8. Horvath, A., Miskolczi, E., Urbanyi, B. 2003. Cryopreservation of common carp sperm. Aquat. Living Resour.,16: 457-460.
  9. Lahnsteiner, F., Patzner, R.A., Weismann, T. 1993. Energy resources of spermatozoa of the rainbow trout, Oncorhynchus mykiss. (Pisces, Teleostei). Reprod. Nutr. Dev., 33: 349-360.
  10. Lahnsteiner, F., Weismann, T., Patzner, R.A.1997. Methanol as cryoprotectant and the suitability of 1.2 ml and 5 ml straws for cryopreservation of sperm from salmonid fishes. Aquacult. Res., 28: 471-479.
  11. Levanduski, M.J., Cloud, J.G. 1988. Rainbow trout (Salmo gairdneri) sperm: effect of non-motile sperm on fertility. Aquacultu