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Journals / Acta Aquatica Turcica / 2020 / Cilt: 16 - Sayı: 1

Effects of Crocus cancellatus subsp. mazziaricus (Herbert) Mathew Plant Extract on the Innate Immune System of European Sea bass (Dicentrarchus labrax, L.1758)

Crocus cancellatus subsp. mazziaricus (Herbert) Mathew Bitki Ekstraktının Avrupa Deniz Levrek Balığı (Dicentrarchus labrax, L.1758) Doğal Bağışıklık Sistemi Üzerine Etkisi

Canan ÖNTAŞ
Gülşen ULUKÖY
Esin BABA
Ramazan MAMMADOV
Pages
148–157
Publication Date
2020-03-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result of plant extract application, the 2 mg/fish group was found to be more effective than the 0.5 mg/fish group.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, Crocus cancellatus subsp. mazziaricus (Herbert) Mathew geophyte plant extract was applied to European sea bass (Dicentrarchus labrax, L. 1758) in two different concentrations (0.5 and 2 mg/fish) by intraperitoneal injection method and its effect on the immune system was investigated.
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Key Findings & Data:After injection on the 7th, 14th, 21st, 28th and 35th days, blood samples were collected from fish.
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Conclusion & Impact:As a result of plant extract application, the 2 mg/fish group was found to be more effective than the 0.5 mg/fish group.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:İmmunostimulant · bitki ekstraktı · bağışıklık sistemi

Abstract

In this study, Crocus cancellatus subsp. mazziaricus (Herbert) Mathew geophyte plant extract was applied to European sea bass (Dicentrarchus labrax, L. 1758) in two different concentrations (0.5 and 2 mg/fish) by intraperitoneal injection method and its effect on the immune system was investigated. After injection on the 7th, 14th, 21st, 28th and 35th days, blood samples were collected from fish. Some of the innate immune related parameters in blood and serum samples such as total leukocyte count, hematocrit level, NBT (+) cell count, lysozyme activity and serum total protein content were determined. C. cancellatus subsp. mazziaricus plant extract treated groups exhibited significant immunostimulatory effect, increased NBT (+) cell count at 21st day, total leucocyte count in 14th day, serum total protein level at 14th and 21st day and serum lysozyme activity at 21st day compared with the control group. As a result of plant extract application, the 2 mg/fish group was found to be more effective than the 0.5 mg/fish group.

Özet

Bu çalışmada Crocus cancellatus subsp. mazziaricus (Herbert) Mathew geofit bitki ekstraktı iki farklı konsantrasyonda (0,5 ve 2 mg/balık) intraperitonal enjeksiyon yöntemiyle Avrupa deniz levreğine (Dicentrarchus labrax, L. 1758) uygulanmış ve bağışıklık sistemine etkisi araştırılmıştır. Enjeksiyon sonrası 7., 14., 21., 28. ve 35. günlerde balıklardan kan örnekleri alınmıştır. Doğal bağışıklık ile ilgili parametrelerden olan toplam lökosit sayısı, hematokrit seviye, NBT(+) hücre sayısı, lizozim aktivitesi ve serum total protein miktarındaki değişimler tespit edilmiştir. Crocus cancellatus subsp. mazziaricus uygulanan deneme gruplarında kontrol grubuna kıyasla doğal bağışıklık sisteminin aktifleşerek, NBT(+) hücre sayısının 21. günde, toplam lökosit sayısının 14.günde, serum total protein miktarının 14. ve 21. günlerde ve serum lizozim aktivitesinin 21. günde en yüksek seviyeye ulaştığı tespit edilmiştir. Sonuç olarak, 2 mg/balık dozunda uygulanan C. cancellatus subsp. mazziaricus ekstraktının 0,5 mg/balık dozuna kıyasla daha etkin olduğu bulunmuştur.

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Cite This Article

ÖNTAŞ, C., ULUKÖY, G., BABA, E., MAMMADOV, R. (2020). Effects of Crocus cancellatus subsp. mazziaricus (Herbert) Mathew Plant Extract on the Innate Immune System of European Sea bass (Dicentrarchus labrax, L.1758). Acta Aquatica Turcica, Cilt: 16 - Sayı: 1, 148–157. https://ajindex.com/articles/18393

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