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Journals / Acta Aquatica Turcica / 2020 / Cilt: 16 - Sayı: 2

Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water

Atık Sudan İzole Edilen Bakteri Suşlarının Biyofilm Oluşumu ve Genişletilmiş Spektrum Beta Laktamaz (ESBL) Üretimi

Hatice Aysun MERCİMEK TAKCI
Pemra BAKIRHAN
Sema GENÇ
Melike KAPTANOĞLU
Pages
170–178
Publication Date
2020-06-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

ESBL and biofilm production provide an important pathogenic character allowing protection against antibiotic treatments to Enterococcus spp. (C2).

📌 3 Key Takeaways

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Objective & Hypothesis:The production extended spectrum beta lactamase (ESBL) and metallo-beta lactamase of strains grown biofilm that were isolated from Kilis State Hospital sewage water were investigated in this study.
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Key Findings & Data:The extended spectrum beta lactamase and metallo-beta lactamase expression of biofilm producing bacteria was tested by using Kirby-Bauer disc diffusion method according to CLSI guidelines. 5 clinical isolates were revealed to be biofilm producer.
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Conclusion & Impact:ESBL and biofilm production provide an important pathogenic character allowing protection against antibiotic treatments to Enterococcus spp. (C2).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Biyofilm · GSBL · metallo-beta laktamaz

Abstract

The production extended spectrum beta lactamase (ESBL) and metallo-beta lactamase of strains grown biofilm that were isolated from Kilis State Hospital sewage water were investigated in this study. Congo red agar, tube adherence, tissue culture plate tests and SEM analysis were performed for the detection of biofilm production. The extended spectrum beta lactamase and metallo-beta lactamase expression of biofilm producing bacteria was tested by using Kirby-Bauer disc diffusion method according to CLSI guidelines. 5 clinical isolates were revealed to be biofilm producer. From of these isolates, Enterococcus spp. (C2) and E. coli strains showed strong biofilm producing. One of Enterococcus isolates, SEM micrograph of C1 was viewed the weak adherence on glass surface. Only one from 5 isolates, Enterococcus spp. (C2) strain was determined to synthesis ESBL enzyme. None of strains was observed to not produce metallo-beta lactamase. ESBL and biofilm production provide an important pathogenic character allowing protection against antibiotic treatments to Enterococcus spp. (C2).

Özet

Bu çalışmada Kilis Devlet Hastanesi kanalizasyon suyundan izole edilen biyofilm oluşturan suşların genişlemiş spektrumlu beta laktamaz ve metallo-beta laktamaz üretimi incelenmiştir. Biyofilm üretiminin belirlenmesi için kongo kırmızısı agar, tüp adherens, doku kültürü plak testleri ve SEM analizi uygulanmıştır. Biyofilm üreten suşların genişlemiş spektrumlu beta laktamaz ve metallo-beta laktamaz ekspresyonu CLSI yönetmeliğine göre Kirby-Bauer disk difüzyon metodu kullanılarak test edilmiştir. 5 klinik izolatın biyofilm üreticisi olduğu belirlenmiştir. Bu izolatlardan Enterococcus spp. (C2) ve E. coli suşları güçlü biyofilm üretmiştir. Enterococcus izolatlarından C1’in SEM mikrografında cam yüzeye zayıf tutunma gözlenmiştir. 5 izolattan yalnızca biri, Enterococcus spp.’nin (C2) GSBL enzimi sentezlediği ortaya konmuştur. Suşlardan hiçbirinin metallo-beta laktamaz üretmediği belirlenmiştir. GSBL ve biyofilm üretimi Enterococcus spp. (C2) suşuna antibiyotik tedavilerine karşı koruyarak önemli bir patojenik karakter sağlamaktadır.

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Cite This Article

TAKCI, H. A. M., BAKIRHAN, P., GENÇ, S., KAPTANOĞLU, M. (2020). Biofilm Formation and Extended Spectrum Beta Lactamase (ESBL) Production of Bacterial Strains Isolated From Sewage Water. Acta Aquatica Turcica, Cilt: 16 - Sayı: 2, 170–178. https://ajindex.com/articles/18364

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References (1)

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