Journals / Turkish Journal of Biology / 2000 / Cilt: 24 - Sayı: 1

Changes in Liver Cytoplasmic Glutathione-S-Transferase and Microsomal Nitrosodimethylamine Demethylase Activities in Chicks Obtained Following Daminozide Treatment

Pages
119–126
Publication Date
2000-01-01
DOI
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⚡ TL;DR(Too Long; Didn't Read)

No significant increase was observed in NDMAd activity (P>0.05) of the group treated with LD 10 dose although NDMAd activity was found to be significantly increased (P

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Objective & Hypothesis:In this study, microsomal nitrosodimethylamine demethylase (NDMAd) and cytoplasmic glutathione-s-tarnsferase (GST) activities in the livers of chicks obtained from the LD 10 (3 mg/ml) and LD 30 (9 mg/ml) doses of daminozide (Succinic acid 2,2-Dimethylhydrazide) injected Ross-Pm-3 fertilized chicken eggs were investigated.
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Key Findings & Data:Spectrophotometric determination of NDMAd activity was performed at 412 nm by the detection of yellow coloured compound formed as a product of the reaction of formaldehyde with Nash reagent.
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Conclusion & Impact:No significant increase was observed in NDMAd activity (P>0.05) of the group treated with LD 10 dose although NDMAd activity was found to be significantly increased (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Daminozide · Nitrosodimethylamine Demethylase · Cytochrome P-450IIE1 Glutathione-S-Transferase.

Abstract

In this study, microsomal nitrosodimethylamine demethylase (NDMAd) and cytoplasmic glutathione-s-tarnsferase (GST) activities in the livers of chicks obtained from the LD 10 (3 mg/ml) and LD 30 (9 mg/ml) doses of daminozide (Succinic acid 2,2-Dimethylhydrazide) injected Ross-Pm-3 fertilized chicken eggs were investigated. Spectrophotometric determination of NDMAd activity was performed at 412 nm by the detection of yellow coloured compound formed as a product of the reaction of formaldehyde with Nash reagent. No significant increase was observed in NDMAd activity (P>0.05) of the group treated with LD 10 dose although NDMAd activity was found to be significantly increased (P

Özet

In this study, microsomal nitrosodimethylamine demethylase (NDMAd) and cytoplasmic glutathione-s-tarnsferase (GST) activities in the livers of chicks obtained from the LD 10 (3 mg/ml) and LD 30 (9 mg/ml) doses of daminozide (Succinic acid 2,2-Dimethylhydrazide) injected Ross-Pm-3 fertilized chicken eggs were investigated. Spectrophotometric determination of NDMAd activity was performed at 412 nm by the detection of yellow coloured compound formed as a product of the reaction of formaldehyde with Nash reagent. No significant increase was observed in NDMAd activity (P>0.05) of the group treated with LD 10 dose although NDMAd activity was found to be significantly increased (P

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Cite This Article

SİLİĞ, Y., ÇELİK, V. K., ATALAY, A. (2000). Changes in Liver Cytoplasmic Glutathione-S-Transferase and Microsomal Nitrosodimethylamine Demethylase Activities in Chicks Obtained Following Daminozide Treatment. Turkish Journal of Biology, Cilt: 24 - Sayı: 1, 119–126. https://ajindex.com/articles/182651

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