SavedAdd your journal

Journals / Turkish Journal of Biology / 2011 / Cilt: 35 - Sayı: 5

Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey

Kadriye İNAN
Sabriye ÇANAKÇI
Ali Osman BELDÜZ
Pages
529–542
Publication Date
2011-05-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The xylanases of I3, CT1Sari, BT2.1, I4.1, ACT2Sari, AC26, and ACT14 were optimally active both at alkaline pH and elevated temperature, and xylanases of I4.1, ACT2Sari, AC26, and ACT14 also were stable at alkaline pH.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Some hot springs located in the west of Turkey were investigated regarding the presence of xylanolytic thermophilic microorganisms.
📊
Key Findings & Data:Based on phenotyping characteristics and 16S rRNA gene sequence analysis, 9 of these xylanolytic isolates belonged to the genus Anoxybacillus, and grew optimally at about 50-60 °C on nutrient agar.
💡
Conclusion & Impact:The xylanases of I3, CT1Sari, BT2.1, I4.1, ACT2Sari, AC26, and ACT14 were optimally active both at alkaline pH and elevated temperature, and xylanases of I4.1, ACT2Sari, AC26, and ACT14 also were stable at alkaline pH.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Alkaline xylanase · thermophiles · Anoxybacillus

Abstract

Some hot springs located in the west of Turkey were investigated regarding the presence of xylanolytic thermophilic microorganisms. Based on phenotyping characteristics and 16S rRNA gene sequence analysis, 9 of these xylanolytic isolates belonged to the genus Anoxybacillus, and grew optimally at about 50-60 °C on nutrient agar. The 16S rRNA gene sequence analysis showed that these isolates resembled the Anoxybacillus species =>97 and these isolates are members of the genus Anoxybacillus. Based on the DNA-DNA hybridization study, SDS-PAGE profile, and biochemical and physiological features, I3, CT1Sari, and BT2.1 isolates are new strains of Anoxybacillus gonensis; I4.2 and B9.3 isolates are new strains of A. voinovskiensis; and I4.1, AC26, ACT14, and ACT2Sari isolates are new strains of A. kestanbolensis. The presence of xylanase activities, their optimum temperature, pH stability, and optimum pH were also investigated. The isolates ACT2Sari and ACT14 had the highest temperature optima (75 °C), and I3, CT1Sari, BT2.1, ACT2Sari, and ACT14 had the highest pH optima (pH 9.0) of xylanase. The xylanases of I3, CT1Sari, BT2.1, I4.1, ACT2Sari, AC26, and ACT14 were optimally active both at alkaline pH and elevated temperature, and xylanases of I4.1, ACT2Sari, AC26, and ACT14 also were stable at alkaline pH.

Özet

Some hot springs located in the west of Turkey were investigated regarding the presence of xylanolytic thermophilic microorganisms. Based on phenotyping characteristics and 16S rRNA gene sequence analysis, 9 of these xylanolytic isolates belonged to the genus Anoxybacillus, and grew optimally at about 50-60 °C on nutrient agar. The 16S rRNA gene sequence analysis showed that these isolates resembled the Anoxybacillus species =>97 and these isolates are members of the genus Anoxybacillus. Based on the DNA-DNA hybridization study, SDS-PAGE profile, and biochemical and physiological features, I3, CT1Sari, and BT2.1 isolates are new strains of Anoxybacillus gonensis; I4.2 and B9.3 isolates are new strains of A. voinovskiensis; and I4.1, AC26, ACT14, and ACT2Sari isolates are new strains of A. kestanbolensis. The presence of xylanase activities, their optimum temperature, pH stability, and optimum pH were also investigated. The isolates ACT2Sari and ACT14 had the highest temperature optima (75 °C), and I3, CT1Sari, BT2.1, ACT2Sari, and ACT14 had the highest pH optima (pH 9.0) of xylanase. The xylanases of I3, CT1Sari, BT2.1, I4.1, ACT2Sari, AC26, and ACT14 were optimally active both at alkaline pH and elevated temperature, and xylanases of I4.1, ACT2Sari, AC26, and ACT14 also were stable at alkaline pH.

🔖

Cite This Article

İNAN, K., ÇANAKÇI, S., BELDÜZ, A. O. (2011). Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. Turkish Journal of Biology, Cilt: 35 - Sayı: 5, 529–542. https://ajindex.com/articles/182427

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Kuisiene N, Raugalas J, Stuknyte M et al. Identifi cation of the genus Geobacillus using genus-specifi c primers, based on the 16S-23S rRNA gene internal transcribed spacer. FEMS 277: 165-172, 2007.
  2. Mamo G, Gashe BA, Gessesse A. A highly thermostable amylase from a newly isolated thermophilic Bacillus sp. WN11. J Appl Microbiol 86: 557-560, 1999.
  3. Hawumba JF, Th eron J, Brözel VS. Th ermophilic protease- producing Geobacillus from Buranga hot springs in Western Uganda. Curr Microbiol 45: 144-150, 2002. Somogyi M. Notes on sugar determination. J Biol Chem 195: 19-23, 1952.
  4. Sonnleitner B, Fiechter A. Advantages of using thermophiles in biotechnological processes: expectations and reality. Trends in Biotechnol 1: 74-80, 1983.
  5. Grant WD, Horikoshi K. Alkaliphiles: ecology and biotechnological applications. In: Herbert RA and Sharp RJ. ed. Molecular Biology and Biotechnology of Extremophiles. New Yoırk: Chapman and Hall; 1992: pp. 143-162. Gessesse A. Purifi cation and properties of two thermostable alkaline xylanases from an alkaliphilic Bacillus sp. Appl Environ Microbiol 64: 3533-3535, 1998.
  6. Mamo G, Hatti-Kaul R, Mattiasson B. A thermostable alkaline active endo-β-1-4-xylanase from Bacillus halodurans S7: Purifi cation and characterization. Enzyme Microb Techn 39: 1492-1498, 2006.
  7. Avcioglu B, Eyupaoglu B, Bakir U. Production and characterization of xylanases of a Bacillus strain isolated from soil. World J Microb Biot 21: 65-68, 2005.
  8. Bergquist PL, Morgan HW. Th e molecular genetics and biotechnological application of enzyme from extremely thermophilic eubacteria. In: Herbert RA and Sharp RJ ed. Molecular Biology and Biotechnology of Extremophiles. Chapman & Hall; 1992: pp. 44-75.
  9. Becker P, Abu-Reesh I, Markossian S et al. Determination of the kinetic parameters during continuous cultivation of the lipase-producing thermophile Bacillus sp. IHI-91 on olive oil. Appl Microbiol Biotechnol 48: 184-190, 1997.
  10. Beg OK, Bhushan B, Kapoor M et al. Production and characterization of thermostable xylanase and pectinase from Streptomyces sp. QG-11-3. J Ind Microbiol Biotechnol 24: 396- 402, 2000.
  11. Lee D, Koh YS, Kim KJ et al. Isolation and characterization of a thermophilic lipase from Bacillus thermoleovorans ID-1. FEMS Microbiol Lett 179: 393-400, 1999.
  12. Touzel JP, O’Donohue M, Debeire P et al. Th ermobacillus xylanilyticus gen. nov., sp. nov., a new aerobic thermophilic xylan-degrading bacterium isolated from farm soil. Int J Syst Evol Microbiol 50: 315-320, 2000. Pikuta