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Journals / Turkish Journal of Biology / 2012 / Cilt: 36 - Sayı: 1

Determination of mutagenic potencies of aqueous extracts of Thermopsis turcica by Ames test

Recep LİMAN
Yasin EREN
Dilek AKYIL
Muhsin KONUK
Pages
85–92
Publication Date
2012-01-01
DOI
—
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⚡ TL;DR(Too Long; Didn't Read)

The results showed that all of the aqueous extracts of T. turcica were mutagenic for S. typhimurium TA98 with S9 mix, and, at a dosage of 3750 µg/plate, the leaf extracts were also found to be mutagenic for S. typhimurium TA102 with or without S9 mix.

📌 3 Key Takeaways

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Objective & Hypothesis:Thermopsis turcica is an endemic species of Turkey popularly known as Eber Sarısı or Piyam; it is a member of the Thermopsis genus, which contains plants considered to be medicinal throughout the world.
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Key Findings & Data:In this study, the mutagenic effect of aqueous extracts obtained from the leaves, root, stem, and flowers of T. turcica were evaluated by the Ames test, using Salmonella typhimurium TA97, TA98, TA100, and TA102 strains with and without an S9 activation system.
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Conclusion & Impact:The results showed that all of the aqueous extracts of T. turcica were mutagenic for S. typhimurium TA98 with S9 mix, and, at a dosage of 3750 µg/plate, the leaf extracts were also found to be mutagenic for S. typhimurium TA102 with or without S9 mix.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Ames test · Salmonella typhimurium · mutagenicity

Abstract

Thermopsis turcica is an endemic species of Turkey popularly known as Eber Sarısı or Piyam; it is a member of the Thermopsis genus, which contains plants considered to be medicinal throughout the world. In this study, the mutagenic effect of aqueous extracts obtained from the leaves, root, stem, and flowers of T. turcica were evaluated by the Ames test, using Salmonella typhimurium TA97, TA98, TA100, and TA102 strains with and without an S9 activation system. The results obtained were analyzed statistically using SPSS for Windows, and the Mann-Whitney test was performed for comparisons. The results showed that all of the aqueous extracts of T. turcica were mutagenic for S. typhimurium TA98 with S9 mix, and, at a dosage of 3750 µg/plate, the leaf extracts were also found to be mutagenic for S. typhimurium TA102 with or without S9 mix. The results further demonstrated that, in relation to the concentration, leaf extracts of T. turcica increased the frequency of reverse mutation in S. typhimurium TA102 with S9 mix.

Özet

Thermopsis turcica is an endemic species of Turkey popularly known as Eber Sarısı or Piyam; it is a member of the Thermopsis genus, which contains plants considered to be medicinal throughout the world. In this study, the mutagenic effect of aqueous extracts obtained from the leaves, root, stem, and flowers of T. turcica were evaluated by the Ames test, using Salmonella typhimurium TA97, TA98, TA100, and TA102 strains with and without an S9 activation system. The results obtained were analyzed statistically using SPSS for Windows, and the Mann-Whitney test was performed for comparisons. The results showed that all of the aqueous extracts of T. turcica were mutagenic for S. typhimurium TA98 with S9 mix, and, at a dosage of 3750 µg/plate, the leaf extracts were also found to be mutagenic for S. typhimurium TA102 with or without S9 mix. The results further demonstrated that, in relation to the concentration, leaf extracts of T. turcica increased the frequency of reverse mutation in S. typhimurium TA102 with S9 mix.

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Cite This Article

LİMAN, R., EREN, Y., AKYIL, D., KONUK, M. (2012). Determination of mutagenic potencies of aqueous extracts of Thermopsis turcica by Ames test. Turkish Journal of Biology, Cilt: 36 - Sayı: 1, 85–92. https://ajindex.com/articles/182395

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