SavedAdd your journal

Journals / Turkish Journal of Biology / 2011 / Cilt: 35 - Sayı: 5

Acid proteinase enzyme activity in Candida albicans strains: a comparison of spectrophotometry and plate methods

Sevim AKÇAĞLAR
Beyza ENER
Okan TÖRE
Pages
559–567
Publication Date
2011-05-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study indicates that, in terms of proteinase activity, there were no statistically significant differences between strains obtained from infection and colonization sites by either method (P > 0.05).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In recent years, the incidence of fungal infections has been rising all over the world.
📊
Key Findings & Data:Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence.
💡
Conclusion & Impact:This study indicates that, in terms of proteinase activity, there were no statistically significant differences between strains obtained from infection and colonization sites by either method (P > 0.05).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Key words: Candida albicans · secreted aspartyl proteinase (SAP) activity · virulence

Abstract

In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates that, in terms of proteinase activity, there were no statistically significant differences between strains obtained from infection and colonization sites by either method (P > 0.05).

Özet

In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates that, in terms of proteinase activity, there were no statistically significant differences between strains obtained from infection and colonization sites by either method (P > 0.05).

🔖

Cite This Article

AKÇAĞLAR, S., ENER, B., TÖRE, O. (2011). Acid proteinase enzyme activity in Candida albicans strains: a comparison of spectrophotometry and plate methods. Turkish Journal of Biology, Cilt: 35 - Sayı: 5, 559–567. https://ajindex.com/articles/182390

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (12)

  1. Navarro-Garcia F, Sánchez M, Nombela C et al. Virulence genes in the pathogenic yeast C. albicans. FEMS Microbiology Reviews 25: 225-268, 2001.
  2. Gokce G, Cerikcioglu N, Yagci A. Acid proteinase, phospholipase and biofi lm production of Candida species isolated from blood cultures. Mycopathologia 164: 265-269, 2007.
  3. Zaugg C, Borg-von Zepelin M, Reichard U et al. Secreted aspartic proteinase family of Candida tropicalis. Infect Immun 69: 405-412, 2001. 4. Pfaller MA. Epidemiology and control of fungal infections. Clin Infect Dis 19: 8-13, 1994.
  4. Edmond MB, Wallace SE, McClish DK et al. Nosocomial bloodstream infections in United States hospitals: a three year analysis. Clin Infect Dis 29: 239-244, 1999.
  5. Wey SB, Mori M, Pfaller MA et al. Hospital acquired candidemia. Th e attributable mortality and excess length of stay. Arch Intern Med 148: 2642-2645, 1998. 7. Yun-Liang Y. Virulence factors of Candida species. J Microbiol Immunol Infect 36: 223-228, 2003.
  6. Kremery V, Barnes AJ. Non-albicans Candida spp. causing fungemia: pathogenicity and antifungal resistance. J Hosp Infect 50: 243-60, 2002. 9. Soll DR. High frequency switching in Candida albicans. Clin Microbiol Rev 5: 183-203, 1992.
  7. Ofek I, Beachey EH. General concepts and principles of bacterial adherence in animal and man. In: Beachey EH. ed. Bacterial Adherence. Chapman and Hall; 1980: pp 1-15. Douglas LJ. Adhesion of pathogenic Candida species to host surfaces. Microbiol Sci 2: 243-247, 1982.
  8. Hube B, Naglik JR. Extracellular hydrolases. In: Calderone RA. ed. Candida and Candidiasis. American Society for Microbiology Press; 2002: pp 107-122. Staib F. Proteolysis and pathogenicity of C. albicans strains. Mycopathologia 37: 345-348, 1969.
  9. Molbay D, Ulgen N, Ener B et al. Proteinase activity of Candida species isolated from various clinical specimens. 7th International Congress of Mycology Division, Prague, Czech Republic, 1994.
  10. Borg M, Rüchel R. Expression of extracellular acid proteinase by proteolytic Candida spp. during experimental infection of oral mucosa. Infect Immun 56: 626-631, 1988.
  11. Schaller M, Bein M, Korting HC et al. Th e secreted aspartyl proteinases Sap1 and Sap2 cause tissue damage in an in vitro model of vaginal candidiasis based on reconstituted human vaginal epithelium. Infect Immun 71: 3227-3234, 2007. Tümbay E. Candida türleri. In Ustaçelebi Ş. ed. Temel ve Klinik Mikrobiyoloji. Günes Kitabevi; 1999: pp. 1081-1086.
  12. Eggimann P, Garbino J, Pittet D. Epidemiology of Candida species infecti