Journals / Turkish Journal of Biology / 2005 / Cilt: 29 - Sayı: 2

Effects of Carbon Sources and Various Chemicals on the Production of a Novel Amylase from a Thermophilic Bacillus sp. K-12

Pages
99–103
Publication Date
2005-02-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

MnSO4, ZnSO4 and EDTA inhibited a-amylase production of Bacillus sp.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The amylase producer thermophilic Bacillus sp.
📊
Key Findings & Data:K-12 was isolated from soil samples from Zeytinli hot spring in Kahramanmaraş.
💡
Conclusion & Impact:MnSO4, ZnSO4 and EDTA inhibited a-amylase production of Bacillus sp.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Thermostable · Amylase · Bacillus

Abstract

The amylase producer thermophilic Bacillus sp. K-12 was isolated from soil samples from Zeytinli hot spring in Kahramanmaraş. Enzyme synthesis occurred at 20-55 ºC with an optimum of 42 ºC. There was a slight variation in amylase synthesis within the pH range 4.5-10.5. Effects of various carbon sources and chemicals on a-amylase production were examined and maximum a-amylase production was obtained in a medium containing 1% starch in 60 h. MnSO4, ZnSO4 and EDTA inhibited a-amylase production of Bacillus sp. K-12.

Özet

The amylase producer thermophilic Bacillus sp. K-12 was isolated from soil samples from Zeytinli hot spring in Kahramanmaraş. Enzyme synthesis occurred at 20-55 ºC with an optimum of 42 ºC. There was a slight variation in amylase synthesis within the pH range 4.5-10.5. Effects of various carbon sources and chemicals on a-amylase production were examined and maximum a-amylase production was obtained in a medium containing 1% starch in 60 h. MnSO4, ZnSO4 and EDTA inhibited a-amylase production of Bacillus sp. K-12.

🔖

Cite This Article

KIRAN, Ö., ÇÖMLEKÇİOĞLU, U., ARIKAN, B. (2005). Effects of Carbon Sources and Various Chemicals on the Production of a Novel Amylase from a Thermophilic Bacillus sp. K-12. Turkish Journal of Biology, Cilt: 29 - Sayı: 2, 99–103. https://ajindex.com/articles/182308

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Aira S, Kilal K, Imanaka A. Cloning and expression of thermostable α-amylase gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis. Appl Environ Microbiol, 1059-1065, 1983.
  2. Albayrak N, Donmez S, Balk M. Toprak ve su örneklerinden amilolitik bakterilerin izolasyonıu ve bazı enzimatik özelliklerinin belirlenmesi. Tr. J. of Biology, 20: 47-54, 1996. Bernfeld P. Amylases αand β. Methods Enzymol, 1: 149-158, 1955.
  3. Lowry OH, Rosebrough NJ, Forr AC et al. Protein measurement with the folin phenol reagent. J. Biol. Chem, 193: 265-275, 1951.
  4. Arikan B, Unaldi N, Coral G et al. Enzymatic properties of novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6. Process Biochemistry, 38: 1397-1403, 2003. Aunstrup K. Production, isolation and economics of extracellular enzymes, In. Wingard JR, Kattchalski-Katzir LB, Golstein L (Eds.). Enzyme Technology, Appl Biochem Bieoeng, vol. 2, Academic Press, NY/San Francisco/London, 1979. Uhlig H. Industrial Enzymes and their Application. PhD, Translated and Updated by Elfride M. Linsmaier-Bednar, Wiley, 1998.
  5. Leveue E, Janacek S, Haye B et al. Thermophilic archeal amylolytic enzymes. Trends in Biotechnol, 7: 49-53, 1989.
  6. Teodoro CES, Martins MLL. Culture conditions for the production of thermostable amylases by Bacillus sp. Brazilian Journal of Microbiology, 31: 1-9, 2000. Sarıkaya E. α-Amilaz üreten bazı Bacillus sufllarının geliflme parametreleri, enzim özellik ve üretim koflullarının optimizasyonu, PhD Ankara Üniversitesi Fen Bilimleri Enstitüsü, 1995.
  7. Kadrekar SN, Ramasarma GB. Amylase and protease production by Bacillus subtilis: Studies on improvement of strain and medium. J. Fd. Sci. Technol, 27: 4-6, 1990.
  8. Aguilar G, Guyot MJ, Aguilar TB et al. Purification and characterization of an extracellular a-amilase produced by Lactobacillus manihotivorans LMG 18010T amylolytic lactic acid bacterium. Enzyme and Microbial Technology, 27: 406-413, 2000.
  9. Sahatta AM, El-Hamahmy AF, Ahmed FH et al. The influence of certain nutritional and environmental factors on the production of amylase enzyme byStreptomyces aureofaciens 77. Journal of Islamic Academy of Sciences, 3:2, 134-138, 1990.
  10. Igarashi K, Hatada Y, Hagihara H et al. Enzymatic properties of a novel liquefying α-amylase from an alkaliphilic Bacillus isolate and entire nucleotide and amino acid sequences. Appl Environ Microbiol, 64: 9, 3282-3289, 1998.
  11. Mamo G, Gessesse A. Purifica