Journals / Turkish Journal of Biology / 2007 / Cilt: 31 - Sayı: 3

Effect of 28-Homobrassinolide on Salinity-Induced Changes in Brassica juncea

Pages
141–146
Publication Date
2007-03-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The study results suggest that HBL can be considered an important plant protector under NaCl stress conditions.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Seedlings of Brassica juncea Czern & Coss cv.
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Key Findings & Data:Varuna generated from seeds soaked in 0, 50, 100, or 150 mM NaCl for 6 h were treated with 0, 10-10, 10-8, or 10-6 M 28-homobrassinolide (HBL) after 14 days of growth.
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Conclusion & Impact:The study results suggest that HBL can be considered an important plant protector under NaCl stress conditions.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Carbonic anhydrase · chlorophyll · nitrate reductase

Abstract

Seedlings of Brassica juncea Czern & Coss cv. Varuna generated from seeds soaked in 0, 50, 100, or 150 mM NaCl for 6 h were treated with 0, 10-10, 10-8, or 10-6 M 28-homobrassinolide (HBL) after 14 days of growth. Plants that received only NaCl treatment exhibited a decrease in nitrate reductase and carbonic anhydrase activity, chlorophyll content, and PN 60 days after sowing (DAS), and decreased seed yield at harvest (140 DAS). Subsequent treatment with HBL significantly increased all of the above parameters. The 10-8 M concentration of HBL generated the best response and also overcame the detrimental effects of NaCl more effectively when NaCl treatment was at the 50 mM level. Tissue proline concentration exhibited a different pattern, increasing in response to both NaCl and HBL treatment; 10-8 M of HBL interacted with 150 mM NaCl most significantly and increased the concentration of proline more than all other treatments. The study results suggest that HBL can be considered an important plant protector under NaCl stress conditions. Abbreviations: CA: carbonic anhydrase; DAS: days after sowing; HBL: 28-homobrassinolide; NR: nitrate reductase; PN: photosynthetic rate

Özet

Seedlings of Brassica juncea Czern & Coss cv. Varuna generated from seeds soaked in 0, 50, 100, or 150 mM NaCl for 6 h were treated with 0, 10-10, 10-8, or 10-6 M 28-homobrassinolide (HBL) after 14 days of growth. Plants that received only NaCl treatment exhibited a decrease in nitrate reductase and carbonic anhydrase activity, chlorophyll content, and PN 60 days after sowing (DAS), and decreased seed yield at harvest (140 DAS). Subsequent treatment with HBL significantly increased all of the above parameters. The 10-8 M concentration of HBL generated the best response and also overcame the detrimental effects of NaCl more effectively when NaCl treatment was at the 50 mM level. Tissue proline concentration exhibited a different pattern, increasing in response to both NaCl and HBL treatment; 10-8 M of HBL interacted with 150 mM NaCl most significantly and increased the concentration of proline more than all other treatments. The study results suggest that HBL can be considered an important plant protector under NaCl stress conditions. Abbreviations: CA: carbonic anhydrase; DAS: days after sowing; HBL: 28-homobrassinolide; NR: nitrate reductase; PN: photosynthetic rate

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Cite This Article

HAYAT, S., ALI, B., HASAN, S. A., AHMAD, A. (2007). Effect of 28-Homobrassinolide on Salinity-Induced Changes in Brassica juncea. Turkish Journal of Biology, Cilt: 31 - Sayı: 3, 141–146. https://ajindex.com/articles/182297

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References (17)

  1. Sasse JM. Physiological actions of brassinosteroids: An update. J Plant Growth Regul 22: 276-288, 2003
  2. Hayat S, Ahmad A. Brassinosteroids: Bioactivity and Crop Productivity. Kluwer Academic Publishers. Dordrecht: 2003
  3. Nunez M, Mazzafera P, Mazorra LM et al. Influence of brassinosteroid analogue on antioxidant enzymes in rice grown in culture medium with NaCl. Biol Plant 47: 67-70, 2003
  4. Jaworski EG. Nitrate reductase assay in intact plant tissues. Biochem Biophys Res Commun 43: 1274-1279, 1971
  5. Dwivedi RS, Randhawa NS. Evolution of a rapid test for the hidden hunger of zinc in plants. Plant Soil 40: 445-451, 1974
  6. Mackinney G. Absorption of light by chlorophyll solutions. J Biol Chem 140: 315-322, 1941
  7. Bates LS, Waldeen RP, Teare ID. Rapid determination of free proline for water stress studies. Plant Soil 39: 205-207, 1973
  8. Gomez KA, Gomez AA. Statistical Procedures for Agricultural Research (2nd ed.), John Wiley and Sons. New York: 1984
  9. Campbell HW. Nitrate reductase structure, function and regulation. Bridging the gap between biochemistry and physiology. Annu Rev Plant Physiol Mol Biol 50: 277-303, 1999
  10. Cramer GR, Lauchli A, Polito VS. Displacement of Ca2+ by Na2+ from the plasmalemma of root cells: A primary response of salt stress. Plant Physiol 79: 207-277, 1985
  11. Solomonson LP, Barber MJ. Assimilatory nitrate reductase: Functional properties and regulation. Annu Rev Plant Physiol Mol Biol 41: 225-253, 1990
  12. Ferrario S, Valadier M, Foyer CH. Overexpression of nitrate reductase in tobacco delays drought-induced decreases in nitrate reductase activity and mRNA. Plant Physiol 117: 293-302, 1998
  13. Mai YY, Lin JM, Zeng XL et al. Effect of homobrassinolide on the activity of nitrate reductase in rice seedlings. Plant Physiol Commun 2: 50-52, 1989
  14. Kalinich JF, Mandava NB, Todhunter JA. Relationship of nucleic acid metabolism on brassinolide-induced responses in beans. J. Plant Physiol 120: 207-214, 1985
  15. Hamada K. Brassinolide in crop cultivation. In: Macgregor, P. ed. Plant Growth Regulators in Agriculture. Food & Fertility Technology Centre of Asian Pacific Region. Taiwan: 1986: pp. 190-196
  16. Tiwari A, Kumar P, Singh S et al. Carbonic anhydrase in relation to higher plants. Photosynthetica 43: 1-9, 2005
  17. Bethke PC, Drew MC. Stomatal and non-stomatal components to inhibition of photosynthesis in leaves of Capsicum annum during progressive exposure to NaCl salinity. Plant Physiol 99: 219-226, 1992